Transcriptome and Parasitome Analysis of Beet Cyst Nematode Heterodera schachtii

Abstract Beet cyst nematodes depend on a set of secretory proteins (effectors) for the induction and maintenance of their syncytial feeding sites in plant roots. In order to understand the relationship between the beet cyst nematode H. schachtii and its host, identification of H. schachtii effectors...

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Autores principales:	Abdelnaser M. Elashry, Samer S. Habash, Paramasivan Vijayapalani, Nahal Brocke-Ahmadinejad, Roman Blümel, Arun Seetharam, Heiko Schoof, Florian M. W. Grundler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/12b3ef6382e345d8af50b0fdd254f4de
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spelling	oai:doaj.org-article:12b3ef6382e345d8af50b0fdd254f4de2021-12-02T10:59:18ZTranscriptome and Parasitome Analysis of Beet Cyst Nematode Heterodera schachtii10.1038/s41598-020-60186-02045-2322https://doaj.org/article/12b3ef6382e345d8af50b0fdd254f4de2020-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-60186-0https://doaj.org/toc/2045-2322Abstract Beet cyst nematodes depend on a set of secretory proteins (effectors) for the induction and maintenance of their syncytial feeding sites in plant roots. In order to understand the relationship between the beet cyst nematode H. schachtii and its host, identification of H. schachtii effectors is crucial and to this end, we sequenced a whole animal pre-infective J2-stage transcriptome in addition to pre- and post-infective J2 gland cell transcriptome using Next Generation Sequencing (NGS) and identified a subset of sequences representing putative effectors. Comparison between the transcriptome of H. schachtii and previously reported related cyst nematodes and root-knot nematodes revealed a subset of esophageal gland related sequences and putative effectors in common across the tested species. Structural and functional annotation of H. schachtii transcriptome led to the identification of nearly 200 putative effectors. Six putative effector expressions were quantified using qPCR and three of them were functionally analyzed using RNAi. Phenotyping of the RNAi nematodes indicated that all tested genes decrease the level of nematodes pathogenicity and/or the average female size, thereby regulating cyst nematode parasitism. These discoveries contribute to further understanding of the cyst nematode parasitism.Abdelnaser M. ElashrySamer S. HabashParamasivan VijayapalaniNahal Brocke-AhmadinejadRoman BlümelArun SeetharamHeiko SchoofFlorian M. W. GrundlerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abdelnaser M. Elashry Samer S. Habash Paramasivan Vijayapalani Nahal Brocke-Ahmadinejad Roman Blümel Arun Seetharam Heiko Schoof Florian M. W. Grundler Transcriptome and Parasitome Analysis of Beet Cyst Nematode Heterodera schachtii
description	Abstract Beet cyst nematodes depend on a set of secretory proteins (effectors) for the induction and maintenance of their syncytial feeding sites in plant roots. In order to understand the relationship between the beet cyst nematode H. schachtii and its host, identification of H. schachtii effectors is crucial and to this end, we sequenced a whole animal pre-infective J2-stage transcriptome in addition to pre- and post-infective J2 gland cell transcriptome using Next Generation Sequencing (NGS) and identified a subset of sequences representing putative effectors. Comparison between the transcriptome of H. schachtii and previously reported related cyst nematodes and root-knot nematodes revealed a subset of esophageal gland related sequences and putative effectors in common across the tested species. Structural and functional annotation of H. schachtii transcriptome led to the identification of nearly 200 putative effectors. Six putative effector expressions were quantified using qPCR and three of them were functionally analyzed using RNAi. Phenotyping of the RNAi nematodes indicated that all tested genes decrease the level of nematodes pathogenicity and/or the average female size, thereby regulating cyst nematode parasitism. These discoveries contribute to further understanding of the cyst nematode parasitism.
format	article
author	Abdelnaser M. Elashry Samer S. Habash Paramasivan Vijayapalani Nahal Brocke-Ahmadinejad Roman Blümel Arun Seetharam Heiko Schoof Florian M. W. Grundler
author_facet	Abdelnaser M. Elashry Samer S. Habash Paramasivan Vijayapalani Nahal Brocke-Ahmadinejad Roman Blümel Arun Seetharam Heiko Schoof Florian M. W. Grundler
author_sort	Abdelnaser M. Elashry
title	Transcriptome and Parasitome Analysis of Beet Cyst Nematode Heterodera schachtii
title_short	Transcriptome and Parasitome Analysis of Beet Cyst Nematode Heterodera schachtii
title_full	Transcriptome and Parasitome Analysis of Beet Cyst Nematode Heterodera schachtii
title_fullStr	Transcriptome and Parasitome Analysis of Beet Cyst Nematode Heterodera schachtii
title_full_unstemmed	Transcriptome and Parasitome Analysis of Beet Cyst Nematode Heterodera schachtii
title_sort	transcriptome and parasitome analysis of beet cyst nematode heterodera schachtii
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/12b3ef6382e345d8af50b0fdd254f4de
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Transcriptome and Parasitome Analysis of Beet Cyst Nematode Heterodera schachtii

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