Multimodal imaging quality control of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells

Abstract Current imaging techniques for the characterization of differentiated corneal limbal stem cells are destructive and cannot be used in eye bank for monitoring the regenerated epithelium in culture. We presented a minimally invasive, multimodal, marker-free imaging method for the investigatio...

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Autores principales:	Marco Lombardo, Sebastiano Serrao, Vanessa Barbaro, Enzo Di Iorio, Giuseppe Lombardo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/12be9e411f7048079b5fa79854cdf48e
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spelling	oai:doaj.org-article:12be9e411f7048079b5fa79854cdf48e2021-12-02T16:08:10ZMultimodal imaging quality control of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells10.1038/s41598-017-05486-82045-2322https://doaj.org/article/12be9e411f7048079b5fa79854cdf48e2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05486-8https://doaj.org/toc/2045-2322Abstract Current imaging techniques for the characterization of differentiated corneal limbal stem cells are destructive and cannot be used in eye bank for monitoring the regenerated epithelium in culture. We presented a minimally invasive, multimodal, marker-free imaging method for the investigation of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells. Two-photon fluorescence and harmonic generation signals were collected from specimens in culture and used for evaluating the structure and morphology of epithelia cultured on two different bio-scaffolds; in addition, donor human corneal tissues were used as controls. The method provided reliable information on the organization of cellular and extracellular components of biomaterial substrates and was highly sensitive to determine differences between the density packing arrangement of epithelial cells of different biomaterials without relying on inferences from exogenous labels. The present minimally invasive standardized quality control methodology can be reliably translated to eye banks and used for monitoring harvested corneal limbal stem cells growth and differentiation in bioengineered materials.Marco LombardoSebastiano SerraoVanessa BarbaroEnzo Di IorioGiuseppe LombardoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marco Lombardo Sebastiano Serrao Vanessa Barbaro Enzo Di Iorio Giuseppe Lombardo Multimodal imaging quality control of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells
description	Abstract Current imaging techniques for the characterization of differentiated corneal limbal stem cells are destructive and cannot be used in eye bank for monitoring the regenerated epithelium in culture. We presented a minimally invasive, multimodal, marker-free imaging method for the investigation of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells. Two-photon fluorescence and harmonic generation signals were collected from specimens in culture and used for evaluating the structure and morphology of epithelia cultured on two different bio-scaffolds; in addition, donor human corneal tissues were used as controls. The method provided reliable information on the organization of cellular and extracellular components of biomaterial substrates and was highly sensitive to determine differences between the density packing arrangement of epithelial cells of different biomaterials without relying on inferences from exogenous labels. The present minimally invasive standardized quality control methodology can be reliably translated to eye banks and used for monitoring harvested corneal limbal stem cells growth and differentiation in bioengineered materials.
format	article
author	Marco Lombardo Sebastiano Serrao Vanessa Barbaro Enzo Di Iorio Giuseppe Lombardo
author_facet	Marco Lombardo Sebastiano Serrao Vanessa Barbaro Enzo Di Iorio Giuseppe Lombardo
author_sort	Marco Lombardo
title	Multimodal imaging quality control of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells
title_short	Multimodal imaging quality control of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells
title_full	Multimodal imaging quality control of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells
title_fullStr	Multimodal imaging quality control of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells
title_full_unstemmed	Multimodal imaging quality control of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells
title_sort	multimodal imaging quality control of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/12be9e411f7048079b5fa79854cdf48e
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_version_	1718384577608679424

Multimodal imaging quality control of epithelia regenerated with cultured human donor corneal limbal epithelial stem cells

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