Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization

CRISPR/Cas9-based genome editing enables specific deletion of target genes. Here, Senturket al. develop an inducible editing system by fusing a conditional destabilization domain to Cas9 and demonstrate temporal control of gene editing in response to a synthetic ligand.

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Autores principales:	Serif Senturk, Nitin H. Shirole, Dawid G. Nowak, Vincenzo Corbo, Debjani Pal, Alexander Vaughan, David A. Tuveson, Lloyd C. Trotman, Justin B. Kinney, Raffaella Sordella
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/12bf0bf4ae954de9832a7c856f71605e
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spelling	oai:doaj.org-article:12bf0bf4ae954de9832a7c856f71605e2021-12-02T15:37:04ZRapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization10.1038/ncomms143702041-1723https://doaj.org/article/12bf0bf4ae954de9832a7c856f71605e2017-02-01T00:00:00Zhttps://doi.org/10.1038/ncomms14370https://doaj.org/toc/2041-1723CRISPR/Cas9-based genome editing enables specific deletion of target genes. Here, Senturket al. develop an inducible editing system by fusing a conditional destabilization domain to Cas9 and demonstrate temporal control of gene editing in response to a synthetic ligand.Serif SenturkNitin H. ShiroleDawid G. NowakVincenzo CorboDebjani PalAlexander VaughanDavid A. TuvesonLloyd C. TrotmanJustin B. KinneyRaffaella SordellaNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Serif Senturk Nitin H. Shirole Dawid G. Nowak Vincenzo Corbo Debjani Pal Alexander Vaughan David A. Tuveson Lloyd C. Trotman Justin B. Kinney Raffaella Sordella Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization
description	CRISPR/Cas9-based genome editing enables specific deletion of target genes. Here, Senturket al. develop an inducible editing system by fusing a conditional destabilization domain to Cas9 and demonstrate temporal control of gene editing in response to a synthetic ligand.
format	article
author	Serif Senturk Nitin H. Shirole Dawid G. Nowak Vincenzo Corbo Debjani Pal Alexander Vaughan David A. Tuveson Lloyd C. Trotman Justin B. Kinney Raffaella Sordella
author_facet	Serif Senturk Nitin H. Shirole Dawid G. Nowak Vincenzo Corbo Debjani Pal Alexander Vaughan David A. Tuveson Lloyd C. Trotman Justin B. Kinney Raffaella Sordella
author_sort	Serif Senturk
title	Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization
title_short	Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization
title_full	Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization
title_fullStr	Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization
title_full_unstemmed	Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization
title_sort	rapid and tunable method to temporally control gene editing based on conditional cas9 stabilization
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/12bf0bf4ae954de9832a7c856f71605e
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Rapid and tunable method to temporally control gene editing based on conditional Cas9 stabilization

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