Olive Leaf Extract (OLE) impaired vasopressin-induced aquaporin-2 trafficking through the activation of the calcium-sensing receptor

Abstract Vasopressin (AVP) increases water permeability in the renal collecting duct through the regulation of aquaporin-2 (AQP2) trafficking. Several disorders, including hypertension and inappropriate antidiuretic hormone secretion (SIADH), are associated with abnormalities in water homeostasis. I...

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Autores principales:	Marianna Ranieri, Annarita Di Mise, Mariangela Centrone, Mariagrazia D’Agostino, Stine Julie Tingskov, Maria Venneri, Tommaso Pellegrino, Graziana Difonzo, Francesco Caponio, Rikke Norregaard, Giovanna Valenti, Grazia Tamma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/12cb165d760144b5a15b242e983724db
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spelling	oai:doaj.org-article:12cb165d760144b5a15b242e983724db2021-12-02T13:34:50ZOlive Leaf Extract (OLE) impaired vasopressin-induced aquaporin-2 trafficking through the activation of the calcium-sensing receptor10.1038/s41598-021-83850-52045-2322https://doaj.org/article/12cb165d760144b5a15b242e983724db2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83850-5https://doaj.org/toc/2045-2322Abstract Vasopressin (AVP) increases water permeability in the renal collecting duct through the regulation of aquaporin-2 (AQP2) trafficking. Several disorders, including hypertension and inappropriate antidiuretic hormone secretion (SIADH), are associated with abnormalities in water homeostasis. It has been shown that certain phytocompounds are beneficial to human health. Here, the effects of the Olive Leaf Extract (OLE) have been evaluated using in vitro and in vivo models. Confocal studies showed that OLE prevents the vasopressin induced AQP2 translocation to the plasma membrane in MCD4 cells and rat kidneys. Incubation with OLE decreases the AVP-dependent increase of the osmotic water permeability coefficient (Pf). To elucidate the possible effectors of OLE, intracellular calcium was evaluated. OLE increases the intracellular calcium through the activation of the Calcium Sensing Receptor (CaSR). NPS2143, a selective CaSR inhibitor, abolished the inhibitory effect of OLE on AVP-dependent water permeability. In vivo experiments revealed that treatment with OLE increases the expression of the CaSR mRNA and decreases AQP2 mRNA paralleled by an increase of the AQP2-targeting miRNA-137. Together, these findings suggest that OLE antagonizes vasopressin action through stimulation of the CaSR indicating that this extract may be beneficial to attenuate disorders characterized by abnormal CaSR signaling and affecting renal water reabsorption.Marianna RanieriAnnarita Di MiseMariangela CentroneMariagrazia D’AgostinoStine Julie TingskovMaria VenneriTommaso PellegrinoGraziana DifonzoFrancesco CaponioRikke NorregaardGiovanna ValentiGrazia TammaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marianna Ranieri Annarita Di Mise Mariangela Centrone Mariagrazia D’Agostino Stine Julie Tingskov Maria Venneri Tommaso Pellegrino Graziana Difonzo Francesco Caponio Rikke Norregaard Giovanna Valenti Grazia Tamma Olive Leaf Extract (OLE) impaired vasopressin-induced aquaporin-2 trafficking through the activation of the calcium-sensing receptor
description	Abstract Vasopressin (AVP) increases water permeability in the renal collecting duct through the regulation of aquaporin-2 (AQP2) trafficking. Several disorders, including hypertension and inappropriate antidiuretic hormone secretion (SIADH), are associated with abnormalities in water homeostasis. It has been shown that certain phytocompounds are beneficial to human health. Here, the effects of the Olive Leaf Extract (OLE) have been evaluated using in vitro and in vivo models. Confocal studies showed that OLE prevents the vasopressin induced AQP2 translocation to the plasma membrane in MCD4 cells and rat kidneys. Incubation with OLE decreases the AVP-dependent increase of the osmotic water permeability coefficient (Pf). To elucidate the possible effectors of OLE, intracellular calcium was evaluated. OLE increases the intracellular calcium through the activation of the Calcium Sensing Receptor (CaSR). NPS2143, a selective CaSR inhibitor, abolished the inhibitory effect of OLE on AVP-dependent water permeability. In vivo experiments revealed that treatment with OLE increases the expression of the CaSR mRNA and decreases AQP2 mRNA paralleled by an increase of the AQP2-targeting miRNA-137. Together, these findings suggest that OLE antagonizes vasopressin action through stimulation of the CaSR indicating that this extract may be beneficial to attenuate disorders characterized by abnormal CaSR signaling and affecting renal water reabsorption.
format	article
author	Marianna Ranieri Annarita Di Mise Mariangela Centrone Mariagrazia D’Agostino Stine Julie Tingskov Maria Venneri Tommaso Pellegrino Graziana Difonzo Francesco Caponio Rikke Norregaard Giovanna Valenti Grazia Tamma
author_facet	Marianna Ranieri Annarita Di Mise Mariangela Centrone Mariagrazia D’Agostino Stine Julie Tingskov Maria Venneri Tommaso Pellegrino Graziana Difonzo Francesco Caponio Rikke Norregaard Giovanna Valenti Grazia Tamma
author_sort	Marianna Ranieri
title	Olive Leaf Extract (OLE) impaired vasopressin-induced aquaporin-2 trafficking through the activation of the calcium-sensing receptor
title_short	Olive Leaf Extract (OLE) impaired vasopressin-induced aquaporin-2 trafficking through the activation of the calcium-sensing receptor
title_full	Olive Leaf Extract (OLE) impaired vasopressin-induced aquaporin-2 trafficking through the activation of the calcium-sensing receptor
title_fullStr	Olive Leaf Extract (OLE) impaired vasopressin-induced aquaporin-2 trafficking through the activation of the calcium-sensing receptor
title_full_unstemmed	Olive Leaf Extract (OLE) impaired vasopressin-induced aquaporin-2 trafficking through the activation of the calcium-sensing receptor
title_sort	olive leaf extract (ole) impaired vasopressin-induced aquaporin-2 trafficking through the activation of the calcium-sensing receptor
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/12cb165d760144b5a15b242e983724db
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Olive Leaf Extract (OLE) impaired vasopressin-induced aquaporin-2 trafficking through the activation of the calcium-sensing receptor

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