Analysis of HIV-1 protease gene reveals frequent multiple infections followed by recombination among drug treated individuals living in São Paulo and Santos, Brazil.

The present study investigated the prevalence of HIV-1 multiple infections in a population composed by 47 patients under HAART failure and enrolled at the National DST/AIDS, Program, Ministry of Health, Brazil.Detection of multiple infections was done using a previously published RFLP assay for the...

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Autores principales:	Edsel Renata De Morais Nunes, Jean Paulo Zukurov, Juliana Terzi Maricato, Maria Cecília Araripe Sucupira, Ricardo Sobhie Diaz, Luíz Mário Ramos Janini
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/12cba4e3cb334b54b4d09b0f1f67fe12
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spelling	oai:doaj.org-article:12cba4e3cb334b54b4d09b0f1f67fe122021-11-18T08:38:59ZAnalysis of HIV-1 protease gene reveals frequent multiple infections followed by recombination among drug treated individuals living in São Paulo and Santos, Brazil.1932-620310.1371/journal.pone.0084066https://doaj.org/article/12cba4e3cb334b54b4d09b0f1f67fe122014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24404149/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The present study investigated the prevalence of HIV-1 multiple infections in a population composed by 47 patients under HAART failure and enrolled at the National DST/AIDS, Program, Ministry of Health, Brazil.Detection of multiple infections was done using a previously published RFLP assay for the HIV-1 protease gene, which is able of distinguishing between infections caused by a single or multiple HIV-1 subtypes. Samples with multiple infections were cloned, and sequence data submitted to phylogenetic analysis. We were able to identify 17 HIV-1 multiple infections out of 47 samples. Multiple infections were mostly composed by a mixture of recombinant viruses (94%), with only one case in which protease gene pure subtypes B and F were recovered. This is the first study that reports the prevalence of multiple infections and intersubtype recombinants in a population undergoing HAART in Brazil. Based on the data there was a steep increase of multiple infections after the introduction of the combined antiretroviral therapy in Brazil. Cases of multiple infections may be associated with HIV-1 genetic diversity through recombination allowing for the generation of viruses showing a combination of resistance mutations.Edsel Renata De Morais NunesJean Paulo ZukurovJuliana Terzi MaricatoMaria Cecília Araripe SucupiraRicardo Sobhie DiazLuíz Mário Ramos JaniniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e84066 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Edsel Renata De Morais Nunes Jean Paulo Zukurov Juliana Terzi Maricato Maria Cecília Araripe Sucupira Ricardo Sobhie Diaz Luíz Mário Ramos Janini Analysis of HIV-1 protease gene reveals frequent multiple infections followed by recombination among drug treated individuals living in São Paulo and Santos, Brazil.
description	The present study investigated the prevalence of HIV-1 multiple infections in a population composed by 47 patients under HAART failure and enrolled at the National DST/AIDS, Program, Ministry of Health, Brazil.Detection of multiple infections was done using a previously published RFLP assay for the HIV-1 protease gene, which is able of distinguishing between infections caused by a single or multiple HIV-1 subtypes. Samples with multiple infections were cloned, and sequence data submitted to phylogenetic analysis. We were able to identify 17 HIV-1 multiple infections out of 47 samples. Multiple infections were mostly composed by a mixture of recombinant viruses (94%), with only one case in which protease gene pure subtypes B and F were recovered. This is the first study that reports the prevalence of multiple infections and intersubtype recombinants in a population undergoing HAART in Brazil. Based on the data there was a steep increase of multiple infections after the introduction of the combined antiretroviral therapy in Brazil. Cases of multiple infections may be associated with HIV-1 genetic diversity through recombination allowing for the generation of viruses showing a combination of resistance mutations.
format	article
author	Edsel Renata De Morais Nunes Jean Paulo Zukurov Juliana Terzi Maricato Maria Cecília Araripe Sucupira Ricardo Sobhie Diaz Luíz Mário Ramos Janini
author_facet	Edsel Renata De Morais Nunes Jean Paulo Zukurov Juliana Terzi Maricato Maria Cecília Araripe Sucupira Ricardo Sobhie Diaz Luíz Mário Ramos Janini
author_sort	Edsel Renata De Morais Nunes
title	Analysis of HIV-1 protease gene reveals frequent multiple infections followed by recombination among drug treated individuals living in São Paulo and Santos, Brazil.
title_short	Analysis of HIV-1 protease gene reveals frequent multiple infections followed by recombination among drug treated individuals living in São Paulo and Santos, Brazil.
title_full	Analysis of HIV-1 protease gene reveals frequent multiple infections followed by recombination among drug treated individuals living in São Paulo and Santos, Brazil.
title_fullStr	Analysis of HIV-1 protease gene reveals frequent multiple infections followed by recombination among drug treated individuals living in São Paulo and Santos, Brazil.
title_full_unstemmed	Analysis of HIV-1 protease gene reveals frequent multiple infections followed by recombination among drug treated individuals living in São Paulo and Santos, Brazil.
title_sort	analysis of hiv-1 protease gene reveals frequent multiple infections followed by recombination among drug treated individuals living in são paulo and santos, brazil.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/12cba4e3cb334b54b4d09b0f1f67fe12
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Analysis of HIV-1 protease gene reveals frequent multiple infections followed by recombination among drug treated individuals living in São Paulo and Santos, Brazil.

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