Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling
The presence of inflammation is one of the key factors in the onset and progression of various medical disorders and diseases, making it a crucial challenge for treatment. The purpose of this study is to evaluate antioxidant and anti-inflammatory effects of Vinpocetine, using acute models of infl...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
South Valley University
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/12cd1db3717349d99233f6ed42e6308c |
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| Sumario: | The presence of inflammation is one of the key factors in the onset and progression of various medical
disorders and diseases, making it a crucial challenge for treatment. The purpose of this study is to
evaluate antioxidant and anti-inflammatory effects of Vinpocetine, using acute models of
inflammation; Xylene-, Carrageenan (CGN)-induced inflammation and acetic acid-induced vascularpermeability in mice were used. We also employed molecular docking approach to verify the
underlying mechanism of anti-inflammatory activity of Vinpocetine. In a current investigation,
Vinpocetine showed anti-inflammatory and antioxidant effects on Xylene- and CGN-induced
inflammation in a dose-dependent manner. Vinpocetine reduced inflammatory edema and restored
antioxidant tissue balance, probably via suppression of IL-1β, IL-6, TNF-α, MDA and MPO activity,
and also through increased non-enzymatic antioxidant (GSH) levels in paw tissue. Histopathological
examination showed that Vinpocetine restored normal skin architecture with significant inhibition of
tissue edema, congestion, and cellular infiltration in CGN-challenged paw. Mechanistically,
Vinpocetine showed downregulation the expression of COX-2 protein, in western blot assessment,
that was associated with significant decrease in the level of prostaglandin E 2 (PGE2) levels; a major
metabolic product of COX-2 enzyme. Interestingly, Silico study showed that Vinpocetine has strong
affinity to COX-2, similar to diclofenac, suggested possible mechanism of downregulation of COX2 expression. Therefore, Vinpocetine showed antioxidant and anti-inflammatory responses might, in
part, due to inhibition of COX-2/PGE 2 pathway. |
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