Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling

The presence of inflammation is one of the key factors in the onset and progression of various medical disorders and diseases, making it a crucial challenge for treatment. The purpose of this study is to evaluate antioxidant and anti-inflammatory effects of Vinpocetine, using acute models of infl...

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Autores principales:	Reham Hassan, Khaled A. Nematallah, Dina M.W. Shibat El-hamed, Samy Abdel-Raouf Fahim Morad, Noha Abdelmageed
Formato:	article
Lenguaje:	EN
Publicado:	South Valley University 2021
Materias:	cox-2/pge 2 pathway cytokines oxidative stress vinpocetine Agriculture S Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/12cd1db3717349d99233f6ed42e6308c
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spelling	oai:doaj.org-article:12cd1db3717349d99233f6ed42e6308c2021-12-02T14:57:02ZVinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling10.21608/svu.2021.70729.11192535-18262535-1877https://doaj.org/article/12cd1db3717349d99233f6ed42e6308c2021-09-01T00:00:00Zhttps://svu.journals.ekb.eg/article_193550.htmlhttps://doaj.org/toc/2535-1826https://doaj.org/toc/2535-1877The presence of inflammation is one of the key factors in the onset and progression of various medical disorders and diseases, making it a crucial challenge for treatment. The purpose of this study is to evaluate antioxidant and anti-inflammatory effects of Vinpocetine, using acute models of inflammation; Xylene-, Carrageenan (CGN)-induced inflammation and acetic acid-induced vascularpermeability in mice were used. We also employed molecular docking approach to verify the underlying mechanism of anti-inflammatory activity of Vinpocetine. In a current investigation, Vinpocetine showed anti-inflammatory and antioxidant effects on Xylene- and CGN-induced inflammation in a dose-dependent manner. Vinpocetine reduced inflammatory edema and restored antioxidant tissue balance, probably via suppression of IL-1β, IL-6, TNF-α, MDA and MPO activity, and also through increased non-enzymatic antioxidant (GSH) levels in paw tissue. Histopathological examination showed that Vinpocetine restored normal skin architecture with significant inhibition of tissue edema, congestion, and cellular infiltration in CGN-challenged paw. Mechanistically, Vinpocetine showed downregulation the expression of COX-2 protein, in western blot assessment, that was associated with significant decrease in the level of prostaglandin E 2 (PGE2) levels; a major metabolic product of COX-2 enzyme. Interestingly, Silico study showed that Vinpocetine has strong affinity to COX-2, similar to diclofenac, suggested possible mechanism of downregulation of COX2 expression. Therefore, Vinpocetine showed antioxidant and anti-inflammatory responses might, in part, due to inhibition of COX-2/PGE 2 pathway.Reham HassanKhaled A. NematallahDina M.W. Shibat El-hamedSamy Abdel-Raouf Fahim MoradNoha AbdelmageedSouth Valley Universityarticlecox-2/pge 2 pathwaycytokinesoxidative stressvinpocetineAgricultureSVeterinary medicineSF600-1100ENSVU-International Journal of Veterinary Sciences, Vol 4, Iss 3, Pp 51-69 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cox-2/pge 2 pathway cytokines oxidative stress vinpocetine Agriculture S Veterinary medicine SF600-1100
spellingShingle	cox-2/pge 2 pathway cytokines oxidative stress vinpocetine Agriculture S Veterinary medicine SF600-1100 Reham Hassan Khaled A. Nematallah Dina M.W. Shibat El-hamed Samy Abdel-Raouf Fahim Morad Noha Abdelmageed Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling
description	The presence of inflammation is one of the key factors in the onset and progression of various medical disorders and diseases, making it a crucial challenge for treatment. The purpose of this study is to evaluate antioxidant and anti-inflammatory effects of Vinpocetine, using acute models of inflammation; Xylene-, Carrageenan (CGN)-induced inflammation and acetic acid-induced vascularpermeability in mice were used. We also employed molecular docking approach to verify the underlying mechanism of anti-inflammatory activity of Vinpocetine. In a current investigation, Vinpocetine showed anti-inflammatory and antioxidant effects on Xylene- and CGN-induced inflammation in a dose-dependent manner. Vinpocetine reduced inflammatory edema and restored antioxidant tissue balance, probably via suppression of IL-1β, IL-6, TNF-α, MDA and MPO activity, and also through increased non-enzymatic antioxidant (GSH) levels in paw tissue. Histopathological examination showed that Vinpocetine restored normal skin architecture with significant inhibition of tissue edema, congestion, and cellular infiltration in CGN-challenged paw. Mechanistically, Vinpocetine showed downregulation the expression of COX-2 protein, in western blot assessment, that was associated with significant decrease in the level of prostaglandin E 2 (PGE2) levels; a major metabolic product of COX-2 enzyme. Interestingly, Silico study showed that Vinpocetine has strong affinity to COX-2, similar to diclofenac, suggested possible mechanism of downregulation of COX2 expression. Therefore, Vinpocetine showed antioxidant and anti-inflammatory responses might, in part, due to inhibition of COX-2/PGE 2 pathway.
format	article
author	Reham Hassan Khaled A. Nematallah Dina M.W. Shibat El-hamed Samy Abdel-Raouf Fahim Morad Noha Abdelmageed
author_facet	Reham Hassan Khaled A. Nematallah Dina M.W. Shibat El-hamed Samy Abdel-Raouf Fahim Morad Noha Abdelmageed
author_sort	Reham Hassan
title	Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling
title_short	Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling
title_full	Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling
title_fullStr	Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling
title_full_unstemmed	Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling
title_sort	vinpocetine suppresses inflammatory and oxidative machineries in acute model of inflammation-pivotal role of cox-2 signaling
publisher	South Valley University
publishDate	2021
url	https://doaj.org/article/12cd1db3717349d99233f6ed42e6308c
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_version_	1718389307968847872

Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation-Pivotal Role of COX-2 Signaling

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