Stable Isotopes for Tracing Cardiac Metabolism in Diseases
Although metabolic remodeling during cardiovascular diseases has been well-recognized for decades, the recent development of analytical platforms and mathematical tools has driven the emergence of assessing cardiac metabolism using tracers. Metabolism is a critical component of cellular functions an...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:12dc2236af5342a7a880fc8bf01e24062021-11-11T05:52:52ZStable Isotopes for Tracing Cardiac Metabolism in Diseases2297-055X10.3389/fcvm.2021.734364https://doaj.org/article/12dc2236af5342a7a880fc8bf01e24062021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcvm.2021.734364/fullhttps://doaj.org/toc/2297-055XAlthough metabolic remodeling during cardiovascular diseases has been well-recognized for decades, the recent development of analytical platforms and mathematical tools has driven the emergence of assessing cardiac metabolism using tracers. Metabolism is a critical component of cellular functions and adaptation to stress. The pathogenesis of cardiovascular disease involves metabolic adaptation to maintain cardiac contractile function even in advanced disease stages. Stable-isotope tracer measurements are a powerful tool for measuring flux distributions at the whole organism level and assessing metabolic changes at a systems level in vivo. The goal of this review is to summarize techniques and concepts for in vivo or ex vivo stable isotope labeling in cardiovascular research, to highlight mathematical concepts and their limitations, to describe analytical methods at the tissue and single-cell level, and to discuss opportunities to leverage metabolic models to address important mechanistic questions relevant to all patients with cardiovascular disease.Anja KarlstaedtAnja KarlstaedtFrontiers Media S.A.articlemetabolismstable-isotope tracermetabolic flux analysissystems biologycardiovascular diseaseDiseases of the circulatory (Cardiovascular) systemRC666-701ENFrontiers in Cardiovascular Medicine, Vol 8 (2021) |
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metabolism stable-isotope tracer metabolic flux analysis systems biology cardiovascular disease Diseases of the circulatory (Cardiovascular) system RC666-701 |
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metabolism stable-isotope tracer metabolic flux analysis systems biology cardiovascular disease Diseases of the circulatory (Cardiovascular) system RC666-701 Anja Karlstaedt Anja Karlstaedt Stable Isotopes for Tracing Cardiac Metabolism in Diseases |
| description |
Although metabolic remodeling during cardiovascular diseases has been well-recognized for decades, the recent development of analytical platforms and mathematical tools has driven the emergence of assessing cardiac metabolism using tracers. Metabolism is a critical component of cellular functions and adaptation to stress. The pathogenesis of cardiovascular disease involves metabolic adaptation to maintain cardiac contractile function even in advanced disease stages. Stable-isotope tracer measurements are a powerful tool for measuring flux distributions at the whole organism level and assessing metabolic changes at a systems level in vivo. The goal of this review is to summarize techniques and concepts for in vivo or ex vivo stable isotope labeling in cardiovascular research, to highlight mathematical concepts and their limitations, to describe analytical methods at the tissue and single-cell level, and to discuss opportunities to leverage metabolic models to address important mechanistic questions relevant to all patients with cardiovascular disease. |
| format |
article |
| author |
Anja Karlstaedt Anja Karlstaedt |
| author_facet |
Anja Karlstaedt Anja Karlstaedt |
| author_sort |
Anja Karlstaedt |
| title |
Stable Isotopes for Tracing Cardiac Metabolism in Diseases |
| title_short |
Stable Isotopes for Tracing Cardiac Metabolism in Diseases |
| title_full |
Stable Isotopes for Tracing Cardiac Metabolism in Diseases |
| title_fullStr |
Stable Isotopes for Tracing Cardiac Metabolism in Diseases |
| title_full_unstemmed |
Stable Isotopes for Tracing Cardiac Metabolism in Diseases |
| title_sort |
stable isotopes for tracing cardiac metabolism in diseases |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/12dc2236af5342a7a880fc8bf01e2406 |
| work_keys_str_mv |
AT anjakarlstaedt stableisotopesfortracingcardiacmetabolismindiseases AT anjakarlstaedt stableisotopesfortracingcardiacmetabolismindiseases |
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