Fluxes of water through aquaporin 9 weaken membrane-cytoskeleton anchorage and promote formation of membrane protrusions.

All modes of cell migration require rapid rearrangements of cell shape, allowing the cell to navigate within narrow spaces in an extracellular matrix. Thus, a highly flexible membrane and a dynamic cytoskeleton are crucial for rapid cell migration. Cytoskeleton dynamics and tension also play instrum...

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Autores principales:	Thommie Karlsson, Anastasia Bolshakova, Marco A O Magalhães, Vesa M Loitto, Karl-Eric Magnusson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/12e3eb332cbd462f901dc694f5ef8610
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spelling	oai:doaj.org-article:12e3eb332cbd462f901dc694f5ef86102021-11-18T07:50:47ZFluxes of water through aquaporin 9 weaken membrane-cytoskeleton anchorage and promote formation of membrane protrusions.1932-620310.1371/journal.pone.0059901https://doaj.org/article/12e3eb332cbd462f901dc694f5ef86102013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23573219/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203All modes of cell migration require rapid rearrangements of cell shape, allowing the cell to navigate within narrow spaces in an extracellular matrix. Thus, a highly flexible membrane and a dynamic cytoskeleton are crucial for rapid cell migration. Cytoskeleton dynamics and tension also play instrumental roles in the formation of different specialized cell membrane protrusions, viz. lamellipodia, filopodia, and membrane blebs. The flux of water through membrane-anchored water channels, known as aquaporins (AQPs) has recently been implicated in the regulation of cell motility, and here we provide novel evidence for the role of AQP9 in the development of various forms of membrane protrusion. Using multiple imaging techniques and cellular models we show that: (i) AQP9 induced and accumulated in filopodia, (ii) AQP9-associated filopodial extensions preceded actin polymerization, which was in turn crucial for their stability and dynamics, and (iii) minute, local reductions in osmolarity immediately initiated small dynamic bleb-like protrusions, the size of which correlated with the reduction in osmotic pressure. Based on this, we present a model for AQP9-induced membrane protrusion, where the interplay of water fluxes through AQP9 and actin dynamics regulate the cellular protrusive and motile activity of cells.Thommie KarlssonAnastasia BolshakovaMarco A O MagalhãesVesa M LoittoKarl-Eric MagnussonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e59901 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thommie Karlsson Anastasia Bolshakova Marco A O Magalhães Vesa M Loitto Karl-Eric Magnusson Fluxes of water through aquaporin 9 weaken membrane-cytoskeleton anchorage and promote formation of membrane protrusions.
description	All modes of cell migration require rapid rearrangements of cell shape, allowing the cell to navigate within narrow spaces in an extracellular matrix. Thus, a highly flexible membrane and a dynamic cytoskeleton are crucial for rapid cell migration. Cytoskeleton dynamics and tension also play instrumental roles in the formation of different specialized cell membrane protrusions, viz. lamellipodia, filopodia, and membrane blebs. The flux of water through membrane-anchored water channels, known as aquaporins (AQPs) has recently been implicated in the regulation of cell motility, and here we provide novel evidence for the role of AQP9 in the development of various forms of membrane protrusion. Using multiple imaging techniques and cellular models we show that: (i) AQP9 induced and accumulated in filopodia, (ii) AQP9-associated filopodial extensions preceded actin polymerization, which was in turn crucial for their stability and dynamics, and (iii) minute, local reductions in osmolarity immediately initiated small dynamic bleb-like protrusions, the size of which correlated with the reduction in osmotic pressure. Based on this, we present a model for AQP9-induced membrane protrusion, where the interplay of water fluxes through AQP9 and actin dynamics regulate the cellular protrusive and motile activity of cells.
format	article
author	Thommie Karlsson Anastasia Bolshakova Marco A O Magalhães Vesa M Loitto Karl-Eric Magnusson
author_facet	Thommie Karlsson Anastasia Bolshakova Marco A O Magalhães Vesa M Loitto Karl-Eric Magnusson
author_sort	Thommie Karlsson
title	Fluxes of water through aquaporin 9 weaken membrane-cytoskeleton anchorage and promote formation of membrane protrusions.
title_short	Fluxes of water through aquaporin 9 weaken membrane-cytoskeleton anchorage and promote formation of membrane protrusions.
title_full	Fluxes of water through aquaporin 9 weaken membrane-cytoskeleton anchorage and promote formation of membrane protrusions.
title_fullStr	Fluxes of water through aquaporin 9 weaken membrane-cytoskeleton anchorage and promote formation of membrane protrusions.
title_full_unstemmed	Fluxes of water through aquaporin 9 weaken membrane-cytoskeleton anchorage and promote formation of membrane protrusions.
title_sort	fluxes of water through aquaporin 9 weaken membrane-cytoskeleton anchorage and promote formation of membrane protrusions.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/12e3eb332cbd462f901dc694f5ef8610
work_keys_str_mv	AT thommiekarlsson fluxesofwaterthroughaquaporin9weakenmembranecytoskeletonanchorageandpromoteformationofmembraneprotrusions AT anastasiabolshakova fluxesofwaterthroughaquaporin9weakenmembranecytoskeletonanchorageandpromoteformationofmembraneprotrusions AT marcoaomagalhaes fluxesofwaterthroughaquaporin9weakenmembranecytoskeletonanchorageandpromoteformationofmembraneprotrusions AT vesamloitto fluxesofwaterthroughaquaporin9weakenmembranecytoskeletonanchorageandpromoteformationofmembraneprotrusions AT karlericmagnusson fluxesofwaterthroughaquaporin9weakenmembranecytoskeletonanchorageandpromoteformationofmembraneprotrusions
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Fluxes of water through aquaporin 9 weaken membrane-cytoskeleton anchorage and promote formation of membrane protrusions.

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