Directed evolution of CRISPR-Cas9 to increase its specificity

Undesired off-target effects can hamper the use of CRISPR-Cas9 in therapeutic applications. Here the authors use a directed evolution approach to develop Sniper-Cas9 which combines high specificity with no loss of on-target activity.

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Autores principales:	Jungjoon K. Lee, Euihwan Jeong, Joonsun Lee, Minhee Jung, Eunji Shin, Young-hoon Kim, Kangin Lee, Inyoung Jung, Daesik Kim, Seokjoong Kim, Jin-Soo Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/12e41efab7774824b3a582ae5751b26d
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spelling	oai:doaj.org-article:12e41efab7774824b3a582ae5751b26d2021-12-02T14:40:44ZDirected evolution of CRISPR-Cas9 to increase its specificity10.1038/s41467-018-05477-x2041-1723https://doaj.org/article/12e41efab7774824b3a582ae5751b26d2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05477-xhttps://doaj.org/toc/2041-1723Undesired off-target effects can hamper the use of CRISPR-Cas9 in therapeutic applications. Here the authors use a directed evolution approach to develop Sniper-Cas9 which combines high specificity with no loss of on-target activity.Jungjoon K. LeeEuihwan JeongJoonsun LeeMinhee JungEunji ShinYoung-hoon KimKangin LeeInyoung JungDaesik KimSeokjoong KimJin-Soo KimNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jungjoon K. Lee Euihwan Jeong Joonsun Lee Minhee Jung Eunji Shin Young-hoon Kim Kangin Lee Inyoung Jung Daesik Kim Seokjoong Kim Jin-Soo Kim Directed evolution of CRISPR-Cas9 to increase its specificity
description	Undesired off-target effects can hamper the use of CRISPR-Cas9 in therapeutic applications. Here the authors use a directed evolution approach to develop Sniper-Cas9 which combines high specificity with no loss of on-target activity.
format	article
author	Jungjoon K. Lee Euihwan Jeong Joonsun Lee Minhee Jung Eunji Shin Young-hoon Kim Kangin Lee Inyoung Jung Daesik Kim Seokjoong Kim Jin-Soo Kim
author_facet	Jungjoon K. Lee Euihwan Jeong Joonsun Lee Minhee Jung Eunji Shin Young-hoon Kim Kangin Lee Inyoung Jung Daesik Kim Seokjoong Kim Jin-Soo Kim
author_sort	Jungjoon K. Lee
title	Directed evolution of CRISPR-Cas9 to increase its specificity
title_short	Directed evolution of CRISPR-Cas9 to increase its specificity
title_full	Directed evolution of CRISPR-Cas9 to increase its specificity
title_fullStr	Directed evolution of CRISPR-Cas9 to increase its specificity
title_full_unstemmed	Directed evolution of CRISPR-Cas9 to increase its specificity
title_sort	directed evolution of crispr-cas9 to increase its specificity
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/12e41efab7774824b3a582ae5751b26d
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Directed evolution of CRISPR-Cas9 to increase its specificity

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