Direct detection of diverse metabolic changes in virally transformed and tax-expressing cells by mass spectrometry.

<h4>Background</h4>Viral transformation of a cell starts at the genetic level, followed by changes in the proteome and the metabolome of the host. There is limited information on the broad metabolic changes in HTLV transformed cells.<h4>Methods and principal findings</h4>Here...

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Autores principales: Prabhakar Sripadi, Bindesh Shrestha, Rebecca L Easley, Lawrence Carpio, Kylene Kehn-Hall, Sebastien Chevalier, Renaud Mahieux, Fatah Kashanchi, Akos Vertes
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Publicado: Public Library of Science (PLoS) 2010
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spelling oai:doaj.org-article:12edf388ec634660ae6773106a1bab6b2021-11-18T06:35:22ZDirect detection of diverse metabolic changes in virally transformed and tax-expressing cells by mass spectrometry.1932-620310.1371/journal.pone.0012590https://doaj.org/article/12edf388ec634660ae6773106a1bab6b2010-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20830293/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Viral transformation of a cell starts at the genetic level, followed by changes in the proteome and the metabolome of the host. There is limited information on the broad metabolic changes in HTLV transformed cells.<h4>Methods and principal findings</h4>Here, we report the detection of key changes in metabolites and lipids directly from human T-lymphotropic virus type 1 and type 3 (HTLV1 and HTLV3) transformed, as well as Tax1 and Tax3 expressing cell lines by laser ablation electrospray ionization (LAESI) mass spectrometry (MS). Comparing LAESI-MS spectra of non-HTLV1 transformed and HTLV1 transformed cells revealed that glycerophosphocholine (PC) lipid components were dominant in the non-HTLV1 transformed cells, and PC(O-32:1) and PC(O-34:1) plasmalogens were displaced by PC(30:0) and PC(32:0) species in the HTLV1 transformed cells. In HTLV1 transformed cells, choline, phosphocholine, spermine and glutathione, among others, were downregulated, whereas creatine, dopamine, arginine and AMP were present at higher levels. When comparing metabolite levels between HTLV3 and Tax3 transfected 293T cells, there were a number of common changes observed, including decreased choline, phosphocholine, spermine, homovanillic acid, and glycerophosphocholine and increased spermidine and N-acetyl aspartic acid. These results indicate that the lipid metabolism pathway as well as the creatine and polyamine biosynthesis pathways are commonly deregulated after expression of HTLV3 and Tax3, indicating that the noted changes are likely due to Tax3 expression. N-acetyl aspartic acid is a novel metabolite that is upregulated in all cell types and all conditions tested.<h4>Conclusions and significance</h4>We demonstrate the high throughput in situ metabolite profiling of HTLV transformed and Tax expressing cells, which facilitates the identification of virus-induced perturbations in the biochemical processes of the host cells. We found virus type-specific (HTLV1 vs. HTLV3), expression-specific (Tax1 vs. Tax3) and cell-type-specific (T lymphocytes vs. kidney epithelial cells) changes in the metabolite profiles. The new insight on the affected metabolic pathways can be used to better understand the molecular mechanisms of HTLV induced transformation, which in turn can result in new treatment strategies.Prabhakar SripadiBindesh ShresthaRebecca L EasleyLawrence CarpioKylene Kehn-HallSebastien ChevalierRenaud MahieuxFatah KashanchiAkos VertesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 9, p e12590 (2010)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Prabhakar Sripadi
Bindesh Shrestha
Rebecca L Easley
Lawrence Carpio
Kylene Kehn-Hall
Sebastien Chevalier
Renaud Mahieux
Fatah Kashanchi
Akos Vertes
Direct detection of diverse metabolic changes in virally transformed and tax-expressing cells by mass spectrometry.
description <h4>Background</h4>Viral transformation of a cell starts at the genetic level, followed by changes in the proteome and the metabolome of the host. There is limited information on the broad metabolic changes in HTLV transformed cells.<h4>Methods and principal findings</h4>Here, we report the detection of key changes in metabolites and lipids directly from human T-lymphotropic virus type 1 and type 3 (HTLV1 and HTLV3) transformed, as well as Tax1 and Tax3 expressing cell lines by laser ablation electrospray ionization (LAESI) mass spectrometry (MS). Comparing LAESI-MS spectra of non-HTLV1 transformed and HTLV1 transformed cells revealed that glycerophosphocholine (PC) lipid components were dominant in the non-HTLV1 transformed cells, and PC(O-32:1) and PC(O-34:1) plasmalogens were displaced by PC(30:0) and PC(32:0) species in the HTLV1 transformed cells. In HTLV1 transformed cells, choline, phosphocholine, spermine and glutathione, among others, were downregulated, whereas creatine, dopamine, arginine and AMP were present at higher levels. When comparing metabolite levels between HTLV3 and Tax3 transfected 293T cells, there were a number of common changes observed, including decreased choline, phosphocholine, spermine, homovanillic acid, and glycerophosphocholine and increased spermidine and N-acetyl aspartic acid. These results indicate that the lipid metabolism pathway as well as the creatine and polyamine biosynthesis pathways are commonly deregulated after expression of HTLV3 and Tax3, indicating that the noted changes are likely due to Tax3 expression. N-acetyl aspartic acid is a novel metabolite that is upregulated in all cell types and all conditions tested.<h4>Conclusions and significance</h4>We demonstrate the high throughput in situ metabolite profiling of HTLV transformed and Tax expressing cells, which facilitates the identification of virus-induced perturbations in the biochemical processes of the host cells. We found virus type-specific (HTLV1 vs. HTLV3), expression-specific (Tax1 vs. Tax3) and cell-type-specific (T lymphocytes vs. kidney epithelial cells) changes in the metabolite profiles. The new insight on the affected metabolic pathways can be used to better understand the molecular mechanisms of HTLV induced transformation, which in turn can result in new treatment strategies.
format article
author Prabhakar Sripadi
Bindesh Shrestha
Rebecca L Easley
Lawrence Carpio
Kylene Kehn-Hall
Sebastien Chevalier
Renaud Mahieux
Fatah Kashanchi
Akos Vertes
author_facet Prabhakar Sripadi
Bindesh Shrestha
Rebecca L Easley
Lawrence Carpio
Kylene Kehn-Hall
Sebastien Chevalier
Renaud Mahieux
Fatah Kashanchi
Akos Vertes
author_sort Prabhakar Sripadi
title Direct detection of diverse metabolic changes in virally transformed and tax-expressing cells by mass spectrometry.
title_short Direct detection of diverse metabolic changes in virally transformed and tax-expressing cells by mass spectrometry.
title_full Direct detection of diverse metabolic changes in virally transformed and tax-expressing cells by mass spectrometry.
title_fullStr Direct detection of diverse metabolic changes in virally transformed and tax-expressing cells by mass spectrometry.
title_full_unstemmed Direct detection of diverse metabolic changes in virally transformed and tax-expressing cells by mass spectrometry.
title_sort direct detection of diverse metabolic changes in virally transformed and tax-expressing cells by mass spectrometry.
publisher Public Library of Science (PLoS)
publishDate 2010
url https://doaj.org/article/12edf388ec634660ae6773106a1bab6b
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