Engineered bidirectional promoters enable rapid multi-gene co-expression optimization

Classic monodirectional promoters are of limited use for multiple gene co-expression. Here the authors generate a library of 168 bidirectional promoters for the yeast K. phaffii (syn. P. pastoris) with diverse expression profiles to optimize metabolic pathway design.

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Autores principales:	Thomas Vogl, Thomas Kickenweiz, Julia Pitzer, Lukas Sturmberger, Astrid Weninger, Bradley W. Biggs, Eva-Maria Köhler, Armin Baumschlager, Jasmin Elgin Fischer, Patrick Hyden, Marlies Wagner, Martina Baumann, Nicole Borth, Martina Geier, Parayil Kumaran Ajikumar, Anton Glieder
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/12f9d0ca14df49a782c862f7b33af465
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spelling	oai:doaj.org-article:12f9d0ca14df49a782c862f7b33af4652021-12-02T17:31:42ZEngineered bidirectional promoters enable rapid multi-gene co-expression optimization10.1038/s41467-018-05915-w2041-1723https://doaj.org/article/12f9d0ca14df49a782c862f7b33af4652018-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05915-whttps://doaj.org/toc/2041-1723Classic monodirectional promoters are of limited use for multiple gene co-expression. Here the authors generate a library of 168 bidirectional promoters for the yeast K. phaffii (syn. P. pastoris) with diverse expression profiles to optimize metabolic pathway design.Thomas VoglThomas KickenweizJulia PitzerLukas SturmbergerAstrid WeningerBradley W. BiggsEva-Maria KöhlerArmin BaumschlagerJasmin Elgin FischerPatrick HydenMarlies WagnerMartina BaumannNicole BorthMartina GeierParayil Kumaran AjikumarAnton GliederNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Thomas Vogl Thomas Kickenweiz Julia Pitzer Lukas Sturmberger Astrid Weninger Bradley W. Biggs Eva-Maria Köhler Armin Baumschlager Jasmin Elgin Fischer Patrick Hyden Marlies Wagner Martina Baumann Nicole Borth Martina Geier Parayil Kumaran Ajikumar Anton Glieder Engineered bidirectional promoters enable rapid multi-gene co-expression optimization
description	Classic monodirectional promoters are of limited use for multiple gene co-expression. Here the authors generate a library of 168 bidirectional promoters for the yeast K. phaffii (syn. P. pastoris) with diverse expression profiles to optimize metabolic pathway design.
format	article
author	Thomas Vogl Thomas Kickenweiz Julia Pitzer Lukas Sturmberger Astrid Weninger Bradley W. Biggs Eva-Maria Köhler Armin Baumschlager Jasmin Elgin Fischer Patrick Hyden Marlies Wagner Martina Baumann Nicole Borth Martina Geier Parayil Kumaran Ajikumar Anton Glieder
author_facet	Thomas Vogl Thomas Kickenweiz Julia Pitzer Lukas Sturmberger Astrid Weninger Bradley W. Biggs Eva-Maria Köhler Armin Baumschlager Jasmin Elgin Fischer Patrick Hyden Marlies Wagner Martina Baumann Nicole Borth Martina Geier Parayil Kumaran Ajikumar Anton Glieder
author_sort	Thomas Vogl
title	Engineered bidirectional promoters enable rapid multi-gene co-expression optimization
title_short	Engineered bidirectional promoters enable rapid multi-gene co-expression optimization
title_full	Engineered bidirectional promoters enable rapid multi-gene co-expression optimization
title_fullStr	Engineered bidirectional promoters enable rapid multi-gene co-expression optimization
title_full_unstemmed	Engineered bidirectional promoters enable rapid multi-gene co-expression optimization
title_sort	engineered bidirectional promoters enable rapid multi-gene co-expression optimization
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/12f9d0ca14df49a782c862f7b33af465
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Engineered bidirectional promoters enable rapid multi-gene co-expression optimization

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