The genetic architecture of water-soluble protein content and its genetic relationship to total protein content in soybean

Abstract Water-soluble protein content (WSPC) is a critical factor in both soybean protein quality and functionality. However, the underlying genetic determinants are unclear. Here, we used 219 soybean accessions and 152 recombinant inbred lines genotyped with high-density markers and phenotyped in...

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Autores principales:	Dan Zhang, Haiyan Lü, Shanshan Chu, Huairen Zhang, Hengyou Zhang, Yuming Yang, Hongyan Li, Deyue Yu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/13093da6edc74b0482d3e554f5b155c7
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spelling	oai:doaj.org-article:13093da6edc74b0482d3e554f5b155c72021-12-02T12:32:28ZThe genetic architecture of water-soluble protein content and its genetic relationship to total protein content in soybean10.1038/s41598-017-04685-72045-2322https://doaj.org/article/13093da6edc74b0482d3e554f5b155c72017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04685-7https://doaj.org/toc/2045-2322Abstract Water-soluble protein content (WSPC) is a critical factor in both soybean protein quality and functionality. However, the underlying genetic determinants are unclear. Here, we used 219 soybean accessions and 152 recombinant inbred lines genotyped with high-density markers and phenotyped in multi-environments to dissect the genetic architectures of WSPC and protein content (PC) using single- and multi-locus genome-wide association studies. In the result, a total of 32 significant loci, including 10 novel loci, significantly associated with WSPC and PC across multi-environments were identified, which were subsequently validated by linkage mapping. Among these loci, only four exhibited pleiotropic effects for PC and WSPC, explaining the low correlation coefficient between the two traits. The largest-effect WSPC-specific loci, GqWSPC8, was stably identified across all six environments and tagged to a linkage disequilibrium block comprising two promising candidate genes AAP8 and 2 S albumin, which might contribute to the high level of WSPC in some soybean varieties. In addition, two genes, Glyma.13G123500 and Glyma.13G194400 with relatively high expression levels at seed development stage compared with other tissues were regarded as promising candidates associated with the PC and WSPC, respectively. Our results provide new insights into the genetic basis of WSPC affecting soybean protein quality and yield.Dan ZhangHaiyan LüShanshan ChuHuairen ZhangHengyou ZhangYuming YangHongyan LiDeyue YuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dan Zhang Haiyan Lü Shanshan Chu Huairen Zhang Hengyou Zhang Yuming Yang Hongyan Li Deyue Yu The genetic architecture of water-soluble protein content and its genetic relationship to total protein content in soybean
description	Abstract Water-soluble protein content (WSPC) is a critical factor in both soybean protein quality and functionality. However, the underlying genetic determinants are unclear. Here, we used 219 soybean accessions and 152 recombinant inbred lines genotyped with high-density markers and phenotyped in multi-environments to dissect the genetic architectures of WSPC and protein content (PC) using single- and multi-locus genome-wide association studies. In the result, a total of 32 significant loci, including 10 novel loci, significantly associated with WSPC and PC across multi-environments were identified, which were subsequently validated by linkage mapping. Among these loci, only four exhibited pleiotropic effects for PC and WSPC, explaining the low correlation coefficient between the two traits. The largest-effect WSPC-specific loci, GqWSPC8, was stably identified across all six environments and tagged to a linkage disequilibrium block comprising two promising candidate genes AAP8 and 2 S albumin, which might contribute to the high level of WSPC in some soybean varieties. In addition, two genes, Glyma.13G123500 and Glyma.13G194400 with relatively high expression levels at seed development stage compared with other tissues were regarded as promising candidates associated with the PC and WSPC, respectively. Our results provide new insights into the genetic basis of WSPC affecting soybean protein quality and yield.
format	article
author	Dan Zhang Haiyan Lü Shanshan Chu Huairen Zhang Hengyou Zhang Yuming Yang Hongyan Li Deyue Yu
author_facet	Dan Zhang Haiyan Lü Shanshan Chu Huairen Zhang Hengyou Zhang Yuming Yang Hongyan Li Deyue Yu
author_sort	Dan Zhang
title	The genetic architecture of water-soluble protein content and its genetic relationship to total protein content in soybean
title_short	The genetic architecture of water-soluble protein content and its genetic relationship to total protein content in soybean
title_full	The genetic architecture of water-soluble protein content and its genetic relationship to total protein content in soybean
title_fullStr	The genetic architecture of water-soluble protein content and its genetic relationship to total protein content in soybean
title_full_unstemmed	The genetic architecture of water-soluble protein content and its genetic relationship to total protein content in soybean
title_sort	genetic architecture of water-soluble protein content and its genetic relationship to total protein content in soybean
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/13093da6edc74b0482d3e554f5b155c7
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The genetic architecture of water-soluble protein content and its genetic relationship to total protein content in soybean

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