Detection of icaA and icaD genes of Staphylococcus aureus isolated in cases of bovine mastitis in the Republic of Serbia

Staphylococcus aureus (S. aureus) is the most common agent of contagious mastitis that causes serious health and economic problems. The ability to form biofilms is an important virulence factor of S. aureus for the establishment of persistent infections. This study is aimed to investigate the presen...

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Autores principales:	Suvajdžić Branko, Teodorović Vlado, Vasilev Dragan, Karabasil Neđeljko, Dimitrijević Mirjana, Đorđević Jasna, Katić Vera
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2017
Materias:	biofilm icaa gene icad gene mastitis staphylococcus aureus Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/130950b78ba447dd80c775fffb9f0156
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spelling	oai:doaj.org-article:130950b78ba447dd80c775fffb9f01562021-11-17T21:27:51ZDetection of icaA and icaD genes of Staphylococcus aureus isolated in cases of bovine mastitis in the Republic of Serbia1820-744810.1515/acve-2017-0015https://doaj.org/article/130950b78ba447dd80c775fffb9f01562017-06-01T00:00:00Zhttps://doi.org/10.1515/acve-2017-0015https://doaj.org/toc/1820-7448Staphylococcus aureus (S. aureus) is the most common agent of contagious mastitis that causes serious health and economic problems. The ability to form biofilms is an important virulence factor of S. aureus for the establishment of persistent infections. This study is aimed to investigate the presence of icaA and icaD, two genes of importance for the biofilm formation in S. aureus bovine mastitis isolates. In order to isolate and identify S. aureus, 1555 milk samples were collected from 401 cows, located in different regions of the Republic of Serbia. Using the conventional microbiological methods 100 isolates were characterized as coagulase-positive staphylococci. After primary biochemical identification, the species confirmation of 44 S. aureus isolates was done using MALDI-TOF Mass Spectrometry and PCR technique, targeting the S. aureus-specific nuc gene. Among all investigated S. aureus isolates 25.0% harbored both icaA gene and icaD genes. The presence of icaD gene alone was confirmed in 40.9% of cases totaling icaD positive isolates to 65.9%. The remaining 34.1% of S. aureus isolates were negative for the presence of both genes. The results of the present study indicate the existence of potential biofilm-producer strains in different regions of the Republic of Serbia, both under intensive and semi-extensive cows breeding.Suvajdžić BrankoTeodorović VladoVasilev DraganKarabasil NeđeljkoDimitrijević MirjanaĐorđević JasnaKatić VeraSciendoarticlebiofilmicaa geneicad genemastitisstaphylococcus aureusVeterinary medicineSF600-1100ENActa Veterinaria, Vol 67, Iss 2, Pp 168-177 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	biofilm icaa gene icad gene mastitis staphylococcus aureus Veterinary medicine SF600-1100
spellingShingle	biofilm icaa gene icad gene mastitis staphylococcus aureus Veterinary medicine SF600-1100 Suvajdžić Branko Teodorović Vlado Vasilev Dragan Karabasil Neđeljko Dimitrijević Mirjana Đorđević Jasna Katić Vera Detection of icaA and icaD genes of Staphylococcus aureus isolated in cases of bovine mastitis in the Republic of Serbia
description	Staphylococcus aureus (S. aureus) is the most common agent of contagious mastitis that causes serious health and economic problems. The ability to form biofilms is an important virulence factor of S. aureus for the establishment of persistent infections. This study is aimed to investigate the presence of icaA and icaD, two genes of importance for the biofilm formation in S. aureus bovine mastitis isolates. In order to isolate and identify S. aureus, 1555 milk samples were collected from 401 cows, located in different regions of the Republic of Serbia. Using the conventional microbiological methods 100 isolates were characterized as coagulase-positive staphylococci. After primary biochemical identification, the species confirmation of 44 S. aureus isolates was done using MALDI-TOF Mass Spectrometry and PCR technique, targeting the S. aureus-specific nuc gene. Among all investigated S. aureus isolates 25.0% harbored both icaA gene and icaD genes. The presence of icaD gene alone was confirmed in 40.9% of cases totaling icaD positive isolates to 65.9%. The remaining 34.1% of S. aureus isolates were negative for the presence of both genes. The results of the present study indicate the existence of potential biofilm-producer strains in different regions of the Republic of Serbia, both under intensive and semi-extensive cows breeding.
format	article
author	Suvajdžić Branko Teodorović Vlado Vasilev Dragan Karabasil Neđeljko Dimitrijević Mirjana Đorđević Jasna Katić Vera
author_facet	Suvajdžić Branko Teodorović Vlado Vasilev Dragan Karabasil Neđeljko Dimitrijević Mirjana Đorđević Jasna Katić Vera
author_sort	Suvajdžić Branko
title	Detection of icaA and icaD genes of Staphylococcus aureus isolated in cases of bovine mastitis in the Republic of Serbia
title_short	Detection of icaA and icaD genes of Staphylococcus aureus isolated in cases of bovine mastitis in the Republic of Serbia
title_full	Detection of icaA and icaD genes of Staphylococcus aureus isolated in cases of bovine mastitis in the Republic of Serbia
title_fullStr	Detection of icaA and icaD genes of Staphylococcus aureus isolated in cases of bovine mastitis in the Republic of Serbia
title_full_unstemmed	Detection of icaA and icaD genes of Staphylococcus aureus isolated in cases of bovine mastitis in the Republic of Serbia
title_sort	detection of icaa and icad genes of staphylococcus aureus isolated in cases of bovine mastitis in the republic of serbia
publisher	Sciendo
publishDate	2017
url	https://doaj.org/article/130950b78ba447dd80c775fffb9f0156
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Detection of icaA and icaD genes of Staphylococcus aureus isolated in cases of bovine mastitis in the Republic of Serbia

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