Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.

Pharmaceutical safety testing requires a cheap, fast and highly efficient platform for real-time evaluation of drug toxicity and secondary effects. In this study, we have developed a microfluidic system for phenotype-based evaluation of toxic and teratogenic effects of drugs using zebrafish (Danio r...

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Autores principales:	Yinbao Li, Fan Yang, Zuanguang Chen, Lijuan Shi, Beibei Zhang, Jianbin Pan, Xinchun Li, Duanping Sun, Hongzhi Yang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/130963b23b984bd99824f51d7f2a80ef
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spelling	oai:doaj.org-article:130963b23b984bd99824f51d7f2a80ef2021-11-18T08:23:23ZZebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.1932-620310.1371/journal.pone.0094792https://doaj.org/article/130963b23b984bd99824f51d7f2a80ef2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24733308/?tool=EBIhttps://doaj.org/toc/1932-6203Pharmaceutical safety testing requires a cheap, fast and highly efficient platform for real-time evaluation of drug toxicity and secondary effects. In this study, we have developed a microfluidic system for phenotype-based evaluation of toxic and teratogenic effects of drugs using zebrafish (Danio rerio) embryos and larvae as the model organism. The microfluidic chip is composed of two independent functional units, enabling the assessment of zebrafish embryos and larvae. Each unit consists of a fluidic concentration gradient generator and a row of seven culture chambers to accommodate zebrafish. To test the accuracy of this new chip platform, we examined the toxicity and teratogenicity of an anti-asthmatic agent-aminophylline (Apl) on 210 embryos and 210 larvae (10 individuals per chamber). The effect of Apl on zebrafish embryonic development was quantitatively assessed by recording a series of physiological indicators such as heart rate, survival rate, body length and hatch rate. Most importantly, a new index called clonic convulsion rate, combined with mortality was used to evaluate the toxicities of Apl on zebrafish larvae. We found that Apl can induce deformity and cardiovascular toxicity in both zebrafish embryos and larvae. This microdevice is a multiplexed testing apparatus that allows for the examination of indexes beyond toxicity and teratogenicity at the sub-organ and cellular levels and provides a potentially cost-effective and rapid pharmaceutical safety assessment tool.Yinbao LiFan YangZuanguang ChenLijuan ShiBeibei ZhangJianbin PanXinchun LiDuanping SunHongzhi YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e94792 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yinbao Li Fan Yang Zuanguang Chen Lijuan Shi Beibei Zhang Jianbin Pan Xinchun Li Duanping Sun Hongzhi Yang Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.
description	Pharmaceutical safety testing requires a cheap, fast and highly efficient platform for real-time evaluation of drug toxicity and secondary effects. In this study, we have developed a microfluidic system for phenotype-based evaluation of toxic and teratogenic effects of drugs using zebrafish (Danio rerio) embryos and larvae as the model organism. The microfluidic chip is composed of two independent functional units, enabling the assessment of zebrafish embryos and larvae. Each unit consists of a fluidic concentration gradient generator and a row of seven culture chambers to accommodate zebrafish. To test the accuracy of this new chip platform, we examined the toxicity and teratogenicity of an anti-asthmatic agent-aminophylline (Apl) on 210 embryos and 210 larvae (10 individuals per chamber). The effect of Apl on zebrafish embryonic development was quantitatively assessed by recording a series of physiological indicators such as heart rate, survival rate, body length and hatch rate. Most importantly, a new index called clonic convulsion rate, combined with mortality was used to evaluate the toxicities of Apl on zebrafish larvae. We found that Apl can induce deformity and cardiovascular toxicity in both zebrafish embryos and larvae. This microdevice is a multiplexed testing apparatus that allows for the examination of indexes beyond toxicity and teratogenicity at the sub-organ and cellular levels and provides a potentially cost-effective and rapid pharmaceutical safety assessment tool.
format	article
author	Yinbao Li Fan Yang Zuanguang Chen Lijuan Shi Beibei Zhang Jianbin Pan Xinchun Li Duanping Sun Hongzhi Yang
author_facet	Yinbao Li Fan Yang Zuanguang Chen Lijuan Shi Beibei Zhang Jianbin Pan Xinchun Li Duanping Sun Hongzhi Yang
author_sort	Yinbao Li
title	Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.
title_short	Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.
title_full	Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.
title_fullStr	Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.
title_full_unstemmed	Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.
title_sort	zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/130963b23b984bd99824f51d7f2a80ef
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Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.

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