Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.

Pharmaceutical safety testing requires a cheap, fast and highly efficient platform for real-time evaluation of drug toxicity and secondary effects. In this study, we have developed a microfluidic system for phenotype-based evaluation of toxic and teratogenic effects of drugs using zebrafish (Danio r...

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Autores principales: Yinbao Li, Fan Yang, Zuanguang Chen, Lijuan Shi, Beibei Zhang, Jianbin Pan, Xinchun Li, Duanping Sun, Hongzhi Yang
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Publicado: Public Library of Science (PLoS) 2014
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Acceso en línea:https://doaj.org/article/130963b23b984bd99824f51d7f2a80ef
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spelling oai:doaj.org-article:130963b23b984bd99824f51d7f2a80ef2021-11-18T08:23:23ZZebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.1932-620310.1371/journal.pone.0094792https://doaj.org/article/130963b23b984bd99824f51d7f2a80ef2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24733308/?tool=EBIhttps://doaj.org/toc/1932-6203Pharmaceutical safety testing requires a cheap, fast and highly efficient platform for real-time evaluation of drug toxicity and secondary effects. In this study, we have developed a microfluidic system for phenotype-based evaluation of toxic and teratogenic effects of drugs using zebrafish (Danio rerio) embryos and larvae as the model organism. The microfluidic chip is composed of two independent functional units, enabling the assessment of zebrafish embryos and larvae. Each unit consists of a fluidic concentration gradient generator and a row of seven culture chambers to accommodate zebrafish. To test the accuracy of this new chip platform, we examined the toxicity and teratogenicity of an anti-asthmatic agent-aminophylline (Apl) on 210 embryos and 210 larvae (10 individuals per chamber). The effect of Apl on zebrafish embryonic development was quantitatively assessed by recording a series of physiological indicators such as heart rate, survival rate, body length and hatch rate. Most importantly, a new index called clonic convulsion rate, combined with mortality was used to evaluate the toxicities of Apl on zebrafish larvae. We found that Apl can induce deformity and cardiovascular toxicity in both zebrafish embryos and larvae. This microdevice is a multiplexed testing apparatus that allows for the examination of indexes beyond toxicity and teratogenicity at the sub-organ and cellular levels and provides a potentially cost-effective and rapid pharmaceutical safety assessment tool.Yinbao LiFan YangZuanguang ChenLijuan ShiBeibei ZhangJianbin PanXinchun LiDuanping SunHongzhi YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 4, p e94792 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Yinbao Li
Fan Yang
Zuanguang Chen
Lijuan Shi
Beibei Zhang
Jianbin Pan
Xinchun Li
Duanping Sun
Hongzhi Yang
Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.
description Pharmaceutical safety testing requires a cheap, fast and highly efficient platform for real-time evaluation of drug toxicity and secondary effects. In this study, we have developed a microfluidic system for phenotype-based evaluation of toxic and teratogenic effects of drugs using zebrafish (Danio rerio) embryos and larvae as the model organism. The microfluidic chip is composed of two independent functional units, enabling the assessment of zebrafish embryos and larvae. Each unit consists of a fluidic concentration gradient generator and a row of seven culture chambers to accommodate zebrafish. To test the accuracy of this new chip platform, we examined the toxicity and teratogenicity of an anti-asthmatic agent-aminophylline (Apl) on 210 embryos and 210 larvae (10 individuals per chamber). The effect of Apl on zebrafish embryonic development was quantitatively assessed by recording a series of physiological indicators such as heart rate, survival rate, body length and hatch rate. Most importantly, a new index called clonic convulsion rate, combined with mortality was used to evaluate the toxicities of Apl on zebrafish larvae. We found that Apl can induce deformity and cardiovascular toxicity in both zebrafish embryos and larvae. This microdevice is a multiplexed testing apparatus that allows for the examination of indexes beyond toxicity and teratogenicity at the sub-organ and cellular levels and provides a potentially cost-effective and rapid pharmaceutical safety assessment tool.
format article
author Yinbao Li
Fan Yang
Zuanguang Chen
Lijuan Shi
Beibei Zhang
Jianbin Pan
Xinchun Li
Duanping Sun
Hongzhi Yang
author_facet Yinbao Li
Fan Yang
Zuanguang Chen
Lijuan Shi
Beibei Zhang
Jianbin Pan
Xinchun Li
Duanping Sun
Hongzhi Yang
author_sort Yinbao Li
title Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.
title_short Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.
title_full Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.
title_fullStr Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.
title_full_unstemmed Zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.
title_sort zebrafish on a chip: a novel platform for real-time monitoring of drug-induced developmental toxicity.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/130963b23b984bd99824f51d7f2a80ef
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