Regulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons.

GABAA receptors distributed in somatodendritic compartments play critical roles in regulating neuronal activities, including spike timing and firing pattern; however, the properties and functions of GABAA receptors at the axon are still poorly understood. By recording from the cut end (bleb) of the...

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Autores principales:	Yang Xia, Yuan Zhao, Mingpo Yang, Shaoqun Zeng, Yousheng Shu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1310c773685d43e4b409f689dfdba202
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spelling	oai:doaj.org-article:1310c773685d43e4b409f689dfdba2022021-11-11T08:21:05ZRegulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons.1932-620310.1371/journal.pone.0100968https://doaj.org/article/1310c773685d43e4b409f689dfdba2022014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24971996/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203GABAA receptors distributed in somatodendritic compartments play critical roles in regulating neuronal activities, including spike timing and firing pattern; however, the properties and functions of GABAA receptors at the axon are still poorly understood. By recording from the cut end (bleb) of the main axon trunk of layer -5 pyramidal neurons in prefrontal cortical slices, we found that currents evoked by GABA iontophoresis could be blocked by picrotoxin, indicating the expression of GABAA receptors in axons. Stationary noise analysis revealed that single-channel properties of axonal GABAA receptors were similar to those of somatic receptors. Perforated patch recording with gramicidin revealed that the reversal potential of the GABA response was more negative than the resting membrane potential at the axon trunk, suggesting that GABA may hyperpolarize the axonal membrane potential. Further experiments demonstrated that the activation of axonal GABAA receptors regulated the amplitude and duration of action potentials (APs) and decreased the AP-induced Ca2+ transients at the axon. Together, our results indicate that the waveform of axonal APs and the downstream Ca2+ signals are modulated by axonal GABAA receptors.Yang XiaYuan ZhaoMingpo YangShaoqun ZengYousheng ShuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100968 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yang Xia Yuan Zhao Mingpo Yang Shaoqun Zeng Yousheng Shu Regulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons.
description	GABAA receptors distributed in somatodendritic compartments play critical roles in regulating neuronal activities, including spike timing and firing pattern; however, the properties and functions of GABAA receptors at the axon are still poorly understood. By recording from the cut end (bleb) of the main axon trunk of layer -5 pyramidal neurons in prefrontal cortical slices, we found that currents evoked by GABA iontophoresis could be blocked by picrotoxin, indicating the expression of GABAA receptors in axons. Stationary noise analysis revealed that single-channel properties of axonal GABAA receptors were similar to those of somatic receptors. Perforated patch recording with gramicidin revealed that the reversal potential of the GABA response was more negative than the resting membrane potential at the axon trunk, suggesting that GABA may hyperpolarize the axonal membrane potential. Further experiments demonstrated that the activation of axonal GABAA receptors regulated the amplitude and duration of action potentials (APs) and decreased the AP-induced Ca2+ transients at the axon. Together, our results indicate that the waveform of axonal APs and the downstream Ca2+ signals are modulated by axonal GABAA receptors.
format	article
author	Yang Xia Yuan Zhao Mingpo Yang Shaoqun Zeng Yousheng Shu
author_facet	Yang Xia Yuan Zhao Mingpo Yang Shaoqun Zeng Yousheng Shu
author_sort	Yang Xia
title	Regulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons.
title_short	Regulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons.
title_full	Regulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons.
title_fullStr	Regulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons.
title_full_unstemmed	Regulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons.
title_sort	regulation of action potential waveforms by axonal gabaa receptors in cortical pyramidal neurons.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/1310c773685d43e4b409f689dfdba202
work_keys_str_mv	AT yangxia regulationofactionpotentialwaveformsbyaxonalgabaareceptorsincorticalpyramidalneurons AT yuanzhao regulationofactionpotentialwaveformsbyaxonalgabaareceptorsincorticalpyramidalneurons AT mingpoyang regulationofactionpotentialwaveformsbyaxonalgabaareceptorsincorticalpyramidalneurons AT shaoqunzeng regulationofactionpotentialwaveformsbyaxonalgabaareceptorsincorticalpyramidalneurons AT youshengshu regulationofactionpotentialwaveformsbyaxonalgabaareceptorsincorticalpyramidalneurons
_version_	1718439284632977408

Regulation of action potential waveforms by axonal GABAA receptors in cortical pyramidal neurons.

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