Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification

Abstract Single cell genome analysis methods are powerful tools to define features of single cells and to identify differences between them. Since the DNA amount of a single cell is very limited, cellular DNA usually needs to be amplified by whole-genome amplification before being subjected to furth...

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Autores principales:	Christiane Bäumer, Evelyn Fisch, Holger Wedler, Frank Reinecke, Christian Korfhage
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/131231fd4bb247beb4186d5286642f07
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spelling	oai:doaj.org-article:131231fd4bb247beb4186d5286642f072021-12-02T16:08:15ZExploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification10.1038/s41598-018-25895-72045-2322https://doaj.org/article/131231fd4bb247beb4186d5286642f072018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25895-7https://doaj.org/toc/2045-2322Abstract Single cell genome analysis methods are powerful tools to define features of single cells and to identify differences between them. Since the DNA amount of a single cell is very limited, cellular DNA usually needs to be amplified by whole-genome amplification before being subjected to further analysis. A single nucleus only contains two haploid genomes. Thus, any DNA damage that prevents amplification results in loss of damaged DNA sites and induces an amplification bias. Therefore, the assessment of single cell DNA quality is urgently required. As of today, there is no simple method to determine the quality of a single cell DNA in a manner that will still retain the entire cellular DNA for amplification and downstream analysis. Here, we describe a method for whole-genome amplification with simultaneous quality control of single cell DNA by using a competitive spike-in DNA template.Christiane BäumerEvelyn FischHolger WedlerFrank ReineckeChristian KorfhageNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christiane Bäumer Evelyn Fisch Holger Wedler Frank Reinecke Christian Korfhage Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification
description	Abstract Single cell genome analysis methods are powerful tools to define features of single cells and to identify differences between them. Since the DNA amount of a single cell is very limited, cellular DNA usually needs to be amplified by whole-genome amplification before being subjected to further analysis. A single nucleus only contains two haploid genomes. Thus, any DNA damage that prevents amplification results in loss of damaged DNA sites and induces an amplification bias. Therefore, the assessment of single cell DNA quality is urgently required. As of today, there is no simple method to determine the quality of a single cell DNA in a manner that will still retain the entire cellular DNA for amplification and downstream analysis. Here, we describe a method for whole-genome amplification with simultaneous quality control of single cell DNA by using a competitive spike-in DNA template.
format	article
author	Christiane Bäumer Evelyn Fisch Holger Wedler Frank Reinecke Christian Korfhage
author_facet	Christiane Bäumer Evelyn Fisch Holger Wedler Frank Reinecke Christian Korfhage
author_sort	Christiane Bäumer
title	Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification
title_short	Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification
title_full	Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification
title_fullStr	Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification
title_full_unstemmed	Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification
title_sort	exploring dna quality of single cells for genome analysis with simultaneous whole-genome amplification
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/131231fd4bb247beb4186d5286642f07
work_keys_str_mv	AT christianebaumer exploringdnaqualityofsinglecellsforgenomeanalysiswithsimultaneouswholegenomeamplification AT evelynfisch exploringdnaqualityofsinglecellsforgenomeanalysiswithsimultaneouswholegenomeamplification AT holgerwedler exploringdnaqualityofsinglecellsforgenomeanalysiswithsimultaneouswholegenomeamplification AT frankreinecke exploringdnaqualityofsinglecellsforgenomeanalysiswithsimultaneouswholegenomeamplification AT christiankorfhage exploringdnaqualityofsinglecellsforgenomeanalysiswithsimultaneouswholegenomeamplification
_version_	1718384514588213248

Exploring DNA quality of single cells for genome analysis with simultaneous whole-genome amplification

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