The effect of tacrolimus-containing polyethylene glycol-modified maghemite nanospheres on reducing oxidative stress and accelerating the healing spinal cord injury of rats based on increasing M2 macrophages

One of the key factors in repairing spinal cord injury is reducing inflammation and oxidative stress. So, it was tried to increase the growth of neurons more rapidly by enhancing the macrophage M2 and reducing the M1 to M2 ratio by using the possible role of tacrolimus (TAC) loaded on polyethylene g...

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Autores principales:	Jing Wang, Tuxiu Xie, Xiaobing Long, Rukai Gao, Lulu Kang, Qingyuan Wang, Jie Jiang, Lu Ye, Jingjun Lyu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Spinal cord injury Tacrolimus Maghemite nanosphere Oxidative stress Macrophages Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/131560fcc067444f869fae6ffd1c7def
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spelling	oai:doaj.org-article:131560fcc067444f869fae6ffd1c7def2021-11-24T04:27:38ZThe effect of tacrolimus-containing polyethylene glycol-modified maghemite nanospheres on reducing oxidative stress and accelerating the healing spinal cord injury of rats based on increasing M2 macrophages1878-535210.1016/j.arabjc.2021.103534https://doaj.org/article/131560fcc067444f869fae6ffd1c7def2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1878535221005499https://doaj.org/toc/1878-5352One of the key factors in repairing spinal cord injury is reducing inflammation and oxidative stress. So, it was tried to increase the growth of neurons more rapidly by enhancing the macrophage M2 and reducing the M1 to M2 ratio by using the possible role of tacrolimus (TAC) loaded on polyethylene glycol-modified maghemite nanospheres (PEG-MNs). In this regard, after designing PEG-MNs-TAC in a three-step process and examining their properties through TEM, DLS and XRD analyses. A toxicity assay was then performed by using MTT test. To investigate the healing role of TAC on damaged tissue, a rat model was used to induce injury in vertebral T6 through physical injury. Then, rats were allocated into five groups: negative control, MNs, PEG-MNs , TAC (and PEG-MNs-TAC. Next, motor scale index, behavioral test, macrophage markers expression, concentration of IL-6, IL-2 and TNF-α, and oxidative stress on rats were analyzed. The results revealed that TAC and PEG-MNs-TAC significantly improved mobility and neuropathic tests. Furthermore, the decrease in TNF-α, IL-6, and IL-2 along with the decrease in ROS levels in the injured spinal cord were considerable in both TAC- and PEG-MNs-TAC-treated groups. Nonetheless, the PEG-MNs-TAC exposed more favorable outcomes compared to TAC. Overall, PEG-MNs-TAC could provide a new treatment for damaged spinal cord care by reducing oxidative stress and decreasing M1 to M2 ratio.Jing WangTuxiu XieXiaobing LongRukai GaoLulu KangQingyuan WangJie JiangLu YeJingjun LyuElsevierarticleSpinal cord injuryTacrolimusMaghemite nanosphereOxidative stressMacrophagesChemistryQD1-999ENArabian Journal of Chemistry, Vol 15, Iss 1, Pp 103534- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Spinal cord injury Tacrolimus Maghemite nanosphere Oxidative stress Macrophages Chemistry QD1-999
spellingShingle	Spinal cord injury Tacrolimus Maghemite nanosphere Oxidative stress Macrophages Chemistry QD1-999 Jing Wang Tuxiu Xie Xiaobing Long Rukai Gao Lulu Kang Qingyuan Wang Jie Jiang Lu Ye Jingjun Lyu The effect of tacrolimus-containing polyethylene glycol-modified maghemite nanospheres on reducing oxidative stress and accelerating the healing spinal cord injury of rats based on increasing M2 macrophages
description	One of the key factors in repairing spinal cord injury is reducing inflammation and oxidative stress. So, it was tried to increase the growth of neurons more rapidly by enhancing the macrophage M2 and reducing the M1 to M2 ratio by using the possible role of tacrolimus (TAC) loaded on polyethylene glycol-modified maghemite nanospheres (PEG-MNs). In this regard, after designing PEG-MNs-TAC in a three-step process and examining their properties through TEM, DLS and XRD analyses. A toxicity assay was then performed by using MTT test. To investigate the healing role of TAC on damaged tissue, a rat model was used to induce injury in vertebral T6 through physical injury. Then, rats were allocated into five groups: negative control, MNs, PEG-MNs , TAC (and PEG-MNs-TAC. Next, motor scale index, behavioral test, macrophage markers expression, concentration of IL-6, IL-2 and TNF-α, and oxidative stress on rats were analyzed. The results revealed that TAC and PEG-MNs-TAC significantly improved mobility and neuropathic tests. Furthermore, the decrease in TNF-α, IL-6, and IL-2 along with the decrease in ROS levels in the injured spinal cord were considerable in both TAC- and PEG-MNs-TAC-treated groups. Nonetheless, the PEG-MNs-TAC exposed more favorable outcomes compared to TAC. Overall, PEG-MNs-TAC could provide a new treatment for damaged spinal cord care by reducing oxidative stress and decreasing M1 to M2 ratio.
format	article
author	Jing Wang Tuxiu Xie Xiaobing Long Rukai Gao Lulu Kang Qingyuan Wang Jie Jiang Lu Ye Jingjun Lyu
author_facet	Jing Wang Tuxiu Xie Xiaobing Long Rukai Gao Lulu Kang Qingyuan Wang Jie Jiang Lu Ye Jingjun Lyu
author_sort	Jing Wang
title	The effect of tacrolimus-containing polyethylene glycol-modified maghemite nanospheres on reducing oxidative stress and accelerating the healing spinal cord injury of rats based on increasing M2 macrophages
title_short	The effect of tacrolimus-containing polyethylene glycol-modified maghemite nanospheres on reducing oxidative stress and accelerating the healing spinal cord injury of rats based on increasing M2 macrophages
title_full	The effect of tacrolimus-containing polyethylene glycol-modified maghemite nanospheres on reducing oxidative stress and accelerating the healing spinal cord injury of rats based on increasing M2 macrophages
title_fullStr	The effect of tacrolimus-containing polyethylene glycol-modified maghemite nanospheres on reducing oxidative stress and accelerating the healing spinal cord injury of rats based on increasing M2 macrophages
title_full_unstemmed	The effect of tacrolimus-containing polyethylene glycol-modified maghemite nanospheres on reducing oxidative stress and accelerating the healing spinal cord injury of rats based on increasing M2 macrophages
title_sort	effect of tacrolimus-containing polyethylene glycol-modified maghemite nanospheres on reducing oxidative stress and accelerating the healing spinal cord injury of rats based on increasing m2 macrophages
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/131560fcc067444f869fae6ffd1c7def
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The effect of tacrolimus-containing polyethylene glycol-modified maghemite nanospheres on reducing oxidative stress and accelerating the healing spinal cord injury of rats based on increasing M2 macrophages

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