BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.

Highly polymorphic markers such as simple sequence repeats (SSRs) or microsatellites are very useful for genetic mapping. In this study novel SSRs were identified in BAC-end sequences (BES) from non-contigged, non-overlapping bacterial artificial clones (BACs) in common bean (Phaseolus vulgaris L.)....

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Autores principales: Matthew Wohlgemuth Blair, Juana Marcela Córdoba, Claritza Muñóz, Deissy K Yuyó
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Publicado: Public Library of Science (PLoS) 2014
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Acceso en línea:https://doaj.org/article/1328115d7a4b41c38d62f5c1607f1419
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spelling oai:doaj.org-article:1328115d7a4b41c38d62f5c1607f14192021-11-25T05:59:21ZBAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.1932-620310.1371/journal.pone.0101873https://doaj.org/article/1328115d7a4b41c38d62f5c1607f14192014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0101873https://doaj.org/toc/1932-6203Highly polymorphic markers such as simple sequence repeats (SSRs) or microsatellites are very useful for genetic mapping. In this study novel SSRs were identified in BAC-end sequences (BES) from non-contigged, non-overlapping bacterial artificial clones (BACs) in common bean (Phaseolus vulgaris L.). These so called "singleton" BACs were from the G19833 Andean gene pool physical map and the new BES-SSR markers were used for the saturation of the inter-gene pool, DOR364×G19833 genetic map. A total of 899 SSR loci were found among the singleton BES, but only 346 loci corresponded to the single di- or tri-nucleotide motifs that were likely to be polymorphic (ATT or AG motifs, principally) and useful for primer design and individual marker mapping. When these novel SSR markers were evaluated in the DOR364×G19833 population parents, 136 markers revealed polymorphism and 106 were mapped. Genetic mapping resulted in a map length of 2291 cM with an average distance between markers of 5.2 cM. The new genetic map was compared to the most recent cytogenetic analysis of common bean chromosomes. We found that the new singleton BES-SSR were helpful in filling peri-centromeric spaces on the cytogenetic map. Short genetic distances between some new singleton-derived BES-SSR markers was common showing suppressed recombination in these regions compared to other parts of the genome. The correlation of singleton-derived SSR marker distribution with other cytogenetic features of the bean genome is discussed.Matthew Wohlgemuth BlairJuana Marcela CórdobaClaritza MuñózDeissy K YuyóPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e101873 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Matthew Wohlgemuth Blair
Juana Marcela Córdoba
Claritza Muñóz
Deissy K Yuyó
BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.
description Highly polymorphic markers such as simple sequence repeats (SSRs) or microsatellites are very useful for genetic mapping. In this study novel SSRs were identified in BAC-end sequences (BES) from non-contigged, non-overlapping bacterial artificial clones (BACs) in common bean (Phaseolus vulgaris L.). These so called "singleton" BACs were from the G19833 Andean gene pool physical map and the new BES-SSR markers were used for the saturation of the inter-gene pool, DOR364×G19833 genetic map. A total of 899 SSR loci were found among the singleton BES, but only 346 loci corresponded to the single di- or tri-nucleotide motifs that were likely to be polymorphic (ATT or AG motifs, principally) and useful for primer design and individual marker mapping. When these novel SSR markers were evaluated in the DOR364×G19833 population parents, 136 markers revealed polymorphism and 106 were mapped. Genetic mapping resulted in a map length of 2291 cM with an average distance between markers of 5.2 cM. The new genetic map was compared to the most recent cytogenetic analysis of common bean chromosomes. We found that the new singleton BES-SSR were helpful in filling peri-centromeric spaces on the cytogenetic map. Short genetic distances between some new singleton-derived BES-SSR markers was common showing suppressed recombination in these regions compared to other parts of the genome. The correlation of singleton-derived SSR marker distribution with other cytogenetic features of the bean genome is discussed.
format article
author Matthew Wohlgemuth Blair
Juana Marcela Córdoba
Claritza Muñóz
Deissy K Yuyó
author_facet Matthew Wohlgemuth Blair
Juana Marcela Córdoba
Claritza Muñóz
Deissy K Yuyó
author_sort Matthew Wohlgemuth Blair
title BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.
title_short BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.
title_full BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.
title_fullStr BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.
title_full_unstemmed BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.
title_sort bac-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/1328115d7a4b41c38d62f5c1607f1419
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