BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.

Highly polymorphic markers such as simple sequence repeats (SSRs) or microsatellites are very useful for genetic mapping. In this study novel SSRs were identified in BAC-end sequences (BES) from non-contigged, non-overlapping bacterial artificial clones (BACs) in common bean (Phaseolus vulgaris L.)....

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Autores principales:	Matthew Wohlgemuth Blair, Juana Marcela Córdoba, Claritza Muñóz, Deissy K Yuyó
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1328115d7a4b41c38d62f5c1607f1419
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spelling	oai:doaj.org-article:1328115d7a4b41c38d62f5c1607f14192021-11-25T05:59:21ZBAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.1932-620310.1371/journal.pone.0101873https://doaj.org/article/1328115d7a4b41c38d62f5c1607f14192014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0101873https://doaj.org/toc/1932-6203Highly polymorphic markers such as simple sequence repeats (SSRs) or microsatellites are very useful for genetic mapping. In this study novel SSRs were identified in BAC-end sequences (BES) from non-contigged, non-overlapping bacterial artificial clones (BACs) in common bean (Phaseolus vulgaris L.). These so called "singleton" BACs were from the G19833 Andean gene pool physical map and the new BES-SSR markers were used for the saturation of the inter-gene pool, DOR364×G19833 genetic map. A total of 899 SSR loci were found among the singleton BES, but only 346 loci corresponded to the single di- or tri-nucleotide motifs that were likely to be polymorphic (ATT or AG motifs, principally) and useful for primer design and individual marker mapping. When these novel SSR markers were evaluated in the DOR364×G19833 population parents, 136 markers revealed polymorphism and 106 were mapped. Genetic mapping resulted in a map length of 2291 cM with an average distance between markers of 5.2 cM. The new genetic map was compared to the most recent cytogenetic analysis of common bean chromosomes. We found that the new singleton BES-SSR were helpful in filling peri-centromeric spaces on the cytogenetic map. Short genetic distances between some new singleton-derived BES-SSR markers was common showing suppressed recombination in these regions compared to other parts of the genome. The correlation of singleton-derived SSR marker distribution with other cytogenetic features of the bean genome is discussed.Matthew Wohlgemuth BlairJuana Marcela CórdobaClaritza MuñózDeissy K YuyóPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e101873 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matthew Wohlgemuth Blair Juana Marcela Córdoba Claritza Muñóz Deissy K Yuyó BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.
description	Highly polymorphic markers such as simple sequence repeats (SSRs) or microsatellites are very useful for genetic mapping. In this study novel SSRs were identified in BAC-end sequences (BES) from non-contigged, non-overlapping bacterial artificial clones (BACs) in common bean (Phaseolus vulgaris L.). These so called "singleton" BACs were from the G19833 Andean gene pool physical map and the new BES-SSR markers were used for the saturation of the inter-gene pool, DOR364×G19833 genetic map. A total of 899 SSR loci were found among the singleton BES, but only 346 loci corresponded to the single di- or tri-nucleotide motifs that were likely to be polymorphic (ATT or AG motifs, principally) and useful for primer design and individual marker mapping. When these novel SSR markers were evaluated in the DOR364×G19833 population parents, 136 markers revealed polymorphism and 106 were mapped. Genetic mapping resulted in a map length of 2291 cM with an average distance between markers of 5.2 cM. The new genetic map was compared to the most recent cytogenetic analysis of common bean chromosomes. We found that the new singleton BES-SSR were helpful in filling peri-centromeric spaces on the cytogenetic map. Short genetic distances between some new singleton-derived BES-SSR markers was common showing suppressed recombination in these regions compared to other parts of the genome. The correlation of singleton-derived SSR marker distribution with other cytogenetic features of the bean genome is discussed.
format	article
author	Matthew Wohlgemuth Blair Juana Marcela Córdoba Claritza Muñóz Deissy K Yuyó
author_facet	Matthew Wohlgemuth Blair Juana Marcela Córdoba Claritza Muñóz Deissy K Yuyó
author_sort	Matthew Wohlgemuth Blair
title	BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.
title_short	BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.
title_full	BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.
title_fullStr	BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.
title_full_unstemmed	BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.
title_sort	bac-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/1328115d7a4b41c38d62f5c1607f1419
work_keys_str_mv	AT matthewwohlgemuthblair bacendmicrosatellitesfromintraandintergenicregionsofthecommonbeangenomeandtheircorrelationwithcytogeneticfeatures AT juanamarcelacordoba bacendmicrosatellitesfromintraandintergenicregionsofthecommonbeangenomeandtheircorrelationwithcytogeneticfeatures AT claritzamunoz bacendmicrosatellitesfromintraandintergenicregionsofthecommonbeangenomeandtheircorrelationwithcytogeneticfeatures AT deissykyuyo bacendmicrosatellitesfromintraandintergenicregionsofthecommonbeangenomeandtheircorrelationwithcytogeneticfeatures
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BAC-end microsatellites from intra and inter-genic regions of the common bean genome and their correlation with cytogenetic features.

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