Direct Target Network of the <named-content content-type="genus-species">Neurospora crassa</named-content> Plant Cell Wall Deconstruction Regulators CLR-1, CLR-2, and XLR-1

ABSTRACT Fungal deconstruction of the plant cell requires a complex orchestration of a wide array of intracellular and extracellular enzymes. In Neurospora crassa, CLR-1, CLR-2, and XLR-1 have been identified as key transcription factors regulating plant cell wall degradation in response to soluble...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	James P. Craig, Samuel T. Coradetti, Trevor L. Starr, N. Louise Glass
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2015
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/132c8bb7325b4a72ae9aa9cf690c1a4f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:132c8bb7325b4a72ae9aa9cf690c1a4f
record_format	dspace
spelling	oai:doaj.org-article:132c8bb7325b4a72ae9aa9cf690c1a4f2021-11-15T15:41:30ZDirect Target Network of the <named-content content-type="genus-species">Neurospora crassa</named-content> Plant Cell Wall Deconstruction Regulators CLR-1, CLR-2, and XLR-110.1128/mBio.01452-152150-7511https://doaj.org/article/132c8bb7325b4a72ae9aa9cf690c1a4f2015-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01452-15https://doaj.org/toc/2150-7511ABSTRACT Fungal deconstruction of the plant cell requires a complex orchestration of a wide array of intracellular and extracellular enzymes. In Neurospora crassa, CLR-1, CLR-2, and XLR-1 have been identified as key transcription factors regulating plant cell wall degradation in response to soluble sugars. The XLR-1 regulon was defined using a constitutively active mutant allele, resulting in hemicellulase gene expression and secretion under noninducing conditions. To define genes directly regulated by CLR-1, CLR-2, and XLR-1, we performed chromatin immunoprecipitation and next-generation sequencing (ChIPseq) on epitope-tagged constructs of these three transcription factors. When N. crassa is exposed to plant cell wall material, CLR-1, CLR-2, and XLR-1 individually bind to the promoters of the most strongly induced genes in their respective regulons. These include promoters of genes encoding cellulases for CLR-1 and CLR-2 (CLR-1/CLR-2) and promoters of genes encoding hemicellulases for XLR-1. CLR-1 bound to its regulon under noninducing conditions; however, this binding alone did not translate into gene expression and enzyme secretion. Motif analysis of the bound genes revealed conserved DNA binding motifs, with the CLR-2 motif matching that of its closest paralog in Saccharomyces cerevisiae, Gal4p. Coimmunoprecipitation studies showed that CLR-1 and CLR-2 act in a homocomplex but not as a CLR-1/CLR-2 heterocomplex. IMPORTANCE Understanding fungal regulation of complex plant cell wall deconstruction pathways in response to multiple environmental signals via interconnected transcriptional circuits provides insight into fungus/plant interactions and eukaryotic nutrient sensing. Coordinated optimization of these regulatory networks is likely required for optimal microbial enzyme production.James P. CraigSamuel T. CoradettiTrevor L. StarrN. Louise GlassAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 6, Iss 5 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 James P. Craig Samuel T. Coradetti Trevor L. Starr N. Louise Glass Direct Target Network of the <named-content content-type="genus-species">Neurospora crassa</named-content> Plant Cell Wall Deconstruction Regulators CLR-1, CLR-2, and XLR-1
description	ABSTRACT Fungal deconstruction of the plant cell requires a complex orchestration of a wide array of intracellular and extracellular enzymes. In Neurospora crassa, CLR-1, CLR-2, and XLR-1 have been identified as key transcription factors regulating plant cell wall degradation in response to soluble sugars. The XLR-1 regulon was defined using a constitutively active mutant allele, resulting in hemicellulase gene expression and secretion under noninducing conditions. To define genes directly regulated by CLR-1, CLR-2, and XLR-1, we performed chromatin immunoprecipitation and next-generation sequencing (ChIPseq) on epitope-tagged constructs of these three transcription factors. When N. crassa is exposed to plant cell wall material, CLR-1, CLR-2, and XLR-1 individually bind to the promoters of the most strongly induced genes in their respective regulons. These include promoters of genes encoding cellulases for CLR-1 and CLR-2 (CLR-1/CLR-2) and promoters of genes encoding hemicellulases for XLR-1. CLR-1 bound to its regulon under noninducing conditions; however, this binding alone did not translate into gene expression and enzyme secretion. Motif analysis of the bound genes revealed conserved DNA binding motifs, with the CLR-2 motif matching that of its closest paralog in Saccharomyces cerevisiae, Gal4p. Coimmunoprecipitation studies showed that CLR-1 and CLR-2 act in a homocomplex but not as a CLR-1/CLR-2 heterocomplex. IMPORTANCE Understanding fungal regulation of complex plant cell wall deconstruction pathways in response to multiple environmental signals via interconnected transcriptional circuits provides insight into fungus/plant interactions and eukaryotic nutrient sensing. Coordinated optimization of these regulatory networks is likely required for optimal microbial enzyme production.
format	article
author	James P. Craig Samuel T. Coradetti Trevor L. Starr N. Louise Glass
author_facet	James P. Craig Samuel T. Coradetti Trevor L. Starr N. Louise Glass
author_sort	James P. Craig
title	Direct Target Network of the <named-content content-type="genus-species">Neurospora crassa</named-content> Plant Cell Wall Deconstruction Regulators CLR-1, CLR-2, and XLR-1
title_short	Direct Target Network of the <named-content content-type="genus-species">Neurospora crassa</named-content> Plant Cell Wall Deconstruction Regulators CLR-1, CLR-2, and XLR-1
title_full	Direct Target Network of the <named-content content-type="genus-species">Neurospora crassa</named-content> Plant Cell Wall Deconstruction Regulators CLR-1, CLR-2, and XLR-1
title_fullStr	Direct Target Network of the <named-content content-type="genus-species">Neurospora crassa</named-content> Plant Cell Wall Deconstruction Regulators CLR-1, CLR-2, and XLR-1
title_full_unstemmed	Direct Target Network of the <named-content content-type="genus-species">Neurospora crassa</named-content> Plant Cell Wall Deconstruction Regulators CLR-1, CLR-2, and XLR-1
title_sort	direct target network of the <named-content content-type="genus-species">neurospora crassa</named-content> plant cell wall deconstruction regulators clr-1, clr-2, and xlr-1
publisher	American Society for Microbiology
publishDate	2015
url	https://doaj.org/article/132c8bb7325b4a72ae9aa9cf690c1a4f
work_keys_str_mv	AT jamespcraig directtargetnetworkofthenamedcontentcontenttypegenusspeciesneurosporacrassanamedcontentplantcellwalldeconstructionregulatorsclr1clr2andxlr1 AT samueltcoradetti directtargetnetworkofthenamedcontentcontenttypegenusspeciesneurosporacrassanamedcontentplantcellwalldeconstructionregulatorsclr1clr2andxlr1 AT trevorlstarr directtargetnetworkofthenamedcontentcontenttypegenusspeciesneurosporacrassanamedcontentplantcellwalldeconstructionregulatorsclr1clr2andxlr1 AT nlouiseglass directtargetnetworkofthenamedcontentcontenttypegenusspeciesneurosporacrassanamedcontentplantcellwalldeconstructionregulatorsclr1clr2andxlr1
_version_	1718427688691040256

Direct Target Network of the <named-content content-type="genus-species">Neurospora crassa</named-content> Plant Cell Wall Deconstruction Regulators CLR-1, CLR-2, and XLR-1

Ejemplares similares