Visualization of a Mammalian Mitochondrion by Coherent X-ray Diffractive Imaging

Visualization of a Mammalian Mitochondrion by Coherent X-ray Diffractive Imaging

Abstract We report a three dimensional (3D) quantitative visualization of a mammalian mitochondrion by coherent x-ray diffractive imaging (CXDI) using synchrotron radiation. The internal structures of a mitochondrion from a mouse embryonic fibroblast cell line (NIH3T3) were visualized by tomographic...

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Autores principales: Yoonhee Kim, Chan Kim, Ou Young Kwon, Daewoong Nam, Sang Soo Kim, Jae Hyun Park, Sunam Kim, Marcus Gallagher-Jones, Yoshiki Kohmura, Tetsuya Ishikawa, Changyong Song, Giyoong Tae, Do Young Noh
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Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/133233ed197a48d6869230bc7add2dc2
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spelling oai:doaj.org-article:133233ed197a48d6869230bc7add2dc22021-12-02T16:06:18ZVisualization of a Mammalian Mitochondrion by Coherent X-ray Diffractive Imaging10.1038/s41598-017-01833-x2045-2322https://doaj.org/article/133233ed197a48d6869230bc7add2dc22017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01833-xhttps://doaj.org/toc/2045-2322Abstract We report a three dimensional (3D) quantitative visualization of a mammalian mitochondrion by coherent x-ray diffractive imaging (CXDI) using synchrotron radiation. The internal structures of a mitochondrion from a mouse embryonic fibroblast cell line (NIH3T3) were visualized by tomographic imaging at approximately 60 nm resolution without the need for sectioning or staining. The overall structure consisted of a high electron density region, composed of the outer and inner membranes and the cristae cluster, which enclosed the lower density mitochondrial matrix. The average mass density of the mitochondrion was about 1.36 g/cm3. Sectioned images of the cristae reveal that they have neither a baffle nor septa shape but were instead irregular. In addition, a high resolution, about 14 nm, 2D projection image was captured of a similar mitochondrion with the aid of strongly scattering Au reference objects. Obtaining 3D images at this improved resolution will allow CXDI to be an effective and nondestructive method for investigating the innate structure of mitochondria and other important life supporting organelles.Yoonhee KimChan KimOu Young KwonDaewoong NamSang Soo KimJae Hyun ParkSunam KimMarcus Gallagher-JonesYoshiki KohmuraTetsuya IshikawaChangyong SongGiyoong TaeDo Young NohNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Yoonhee Kim
Chan Kim
Ou Young Kwon
Daewoong Nam
Sang Soo Kim
Jae Hyun Park
Sunam Kim
Marcus Gallagher-Jones
Yoshiki Kohmura
Tetsuya Ishikawa
Changyong Song
Giyoong Tae
Do Young Noh
Visualization of a Mammalian Mitochondrion by Coherent X-ray Diffractive Imaging
description Abstract We report a three dimensional (3D) quantitative visualization of a mammalian mitochondrion by coherent x-ray diffractive imaging (CXDI) using synchrotron radiation. The internal structures of a mitochondrion from a mouse embryonic fibroblast cell line (NIH3T3) were visualized by tomographic imaging at approximately 60 nm resolution without the need for sectioning or staining. The overall structure consisted of a high electron density region, composed of the outer and inner membranes and the cristae cluster, which enclosed the lower density mitochondrial matrix. The average mass density of the mitochondrion was about 1.36 g/cm3. Sectioned images of the cristae reveal that they have neither a baffle nor septa shape but were instead irregular. In addition, a high resolution, about 14 nm, 2D projection image was captured of a similar mitochondrion with the aid of strongly scattering Au reference objects. Obtaining 3D images at this improved resolution will allow CXDI to be an effective and nondestructive method for investigating the innate structure of mitochondria and other important life supporting organelles.
format article
author Yoonhee Kim
Chan Kim
Ou Young Kwon
Daewoong Nam
Sang Soo Kim
Jae Hyun Park
Sunam Kim
Marcus Gallagher-Jones
Yoshiki Kohmura
Tetsuya Ishikawa
Changyong Song
Giyoong Tae
Do Young Noh
author_facet Yoonhee Kim
Chan Kim
Ou Young Kwon
Daewoong Nam
Sang Soo Kim
Jae Hyun Park
Sunam Kim
Marcus Gallagher-Jones
Yoshiki Kohmura
Tetsuya Ishikawa
Changyong Song
Giyoong Tae
Do Young Noh
author_sort Yoonhee Kim
title Visualization of a Mammalian Mitochondrion by Coherent X-ray Diffractive Imaging
title_short Visualization of a Mammalian Mitochondrion by Coherent X-ray Diffractive Imaging
title_full Visualization of a Mammalian Mitochondrion by Coherent X-ray Diffractive Imaging
title_fullStr Visualization of a Mammalian Mitochondrion by Coherent X-ray Diffractive Imaging
title_full_unstemmed Visualization of a Mammalian Mitochondrion by Coherent X-ray Diffractive Imaging
title_sort visualization of a mammalian mitochondrion by coherent x-ray diffractive imaging
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/133233ed197a48d6869230bc7add2dc2
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