Laser microdissection of sensory organ precursor cells of Drosophila microchaetes.

<h4>Background</h4>In Drosophila, each external sensory organ originates from the division of a unique precursor cell (the sensory organ precursor cell or SOP). Each SOP is specified from a cluster of equivalent cells, called a proneural cluster, all of them competent to become SOP. Alth...

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Autores principales:	Eulalie Buffin, Michel Gho
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1338696233084f9eb490686840af0b44
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spelling	oai:doaj.org-article:1338696233084f9eb490686840af0b442021-11-25T06:25:41ZLaser microdissection of sensory organ precursor cells of Drosophila microchaetes.1932-620310.1371/journal.pone.0009285https://doaj.org/article/1338696233084f9eb490686840af0b442010-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20174573/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>In Drosophila, each external sensory organ originates from the division of a unique precursor cell (the sensory organ precursor cell or SOP). Each SOP is specified from a cluster of equivalent cells, called a proneural cluster, all of them competent to become SOP. Although, it is well known how SOP cells are selected from proneural clusters, little is known about the downstream genes that are regulated during SOP fate specification.<h4>Methodology/principal findings</h4>In order to better understand the mechanism involved in the specification of these precursor cells, we combined laser microdissection, toisolate SOP cells, with transcriptome analysis, to study their RNA profile. Using this procedure, we found that genes that exhibit a 2-fold or greater expression in SOPs versus epithelial cells were mainly associated with Gene Ontology (GO) terms related with cell fate determination and sensory organ specification. Furthermore, we found that several genes such as pebbled/hindsight, scabrous, miranda, senseless, or cut, known to be expressed in SOP cells by independent procedures, are particularly detected in laser microdissected SOP cells rather than in epithelial cells.<h4>Conclusions/significance</h4>These results confirm the feasibility and the specificity of our laser microdissection based procedure. We anticipate that this analysis will give new insight into the selection and specification of neural precursor cells.Eulalie BuffinMichel GhoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 2, p e9285 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eulalie Buffin Michel Gho Laser microdissection of sensory organ precursor cells of Drosophila microchaetes.
description	<h4>Background</h4>In Drosophila, each external sensory organ originates from the division of a unique precursor cell (the sensory organ precursor cell or SOP). Each SOP is specified from a cluster of equivalent cells, called a proneural cluster, all of them competent to become SOP. Although, it is well known how SOP cells are selected from proneural clusters, little is known about the downstream genes that are regulated during SOP fate specification.<h4>Methodology/principal findings</h4>In order to better understand the mechanism involved in the specification of these precursor cells, we combined laser microdissection, toisolate SOP cells, with transcriptome analysis, to study their RNA profile. Using this procedure, we found that genes that exhibit a 2-fold or greater expression in SOPs versus epithelial cells were mainly associated with Gene Ontology (GO) terms related with cell fate determination and sensory organ specification. Furthermore, we found that several genes such as pebbled/hindsight, scabrous, miranda, senseless, or cut, known to be expressed in SOP cells by independent procedures, are particularly detected in laser microdissected SOP cells rather than in epithelial cells.<h4>Conclusions/significance</h4>These results confirm the feasibility and the specificity of our laser microdissection based procedure. We anticipate that this analysis will give new insight into the selection and specification of neural precursor cells.
format	article
author	Eulalie Buffin Michel Gho
author_facet	Eulalie Buffin Michel Gho
author_sort	Eulalie Buffin
title	Laser microdissection of sensory organ precursor cells of Drosophila microchaetes.
title_short	Laser microdissection of sensory organ precursor cells of Drosophila microchaetes.
title_full	Laser microdissection of sensory organ precursor cells of Drosophila microchaetes.
title_fullStr	Laser microdissection of sensory organ precursor cells of Drosophila microchaetes.
title_full_unstemmed	Laser microdissection of sensory organ precursor cells of Drosophila microchaetes.
title_sort	laser microdissection of sensory organ precursor cells of drosophila microchaetes.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/1338696233084f9eb490686840af0b44
work_keys_str_mv	AT eulaliebuffin lasermicrodissectionofsensoryorganprecursorcellsofdrosophilamicrochaetes AT michelgho lasermicrodissectionofsensoryorganprecursorcellsofdrosophilamicrochaetes
_version_	1718413725145235456

Laser microdissection of sensory organ precursor cells of Drosophila microchaetes.

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