Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections

Infections caused by non-tuberculous mycobacteria (NTM) have been a public health problem in recent decades and contribute significantly to the clinical and economic burden globally. The diagnosis of infections is difficult and time-consuming and, in addition, the conventional diagnostics tests do n...

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Autores principales:	Matúš Dohál, Igor Porvazník, Ivan Solovič, Juraj Mokrý
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	whole-genome sequencing non-tuberculous mycobacteria diagnostics Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/134bde557c5349c0bd790343f7e6c9bc
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spelling	oai:doaj.org-article:134bde557c5349c0bd790343f7e6c9bc2021-11-25T18:24:28ZWhole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections10.3390/microorganisms91122372076-2607https://doaj.org/article/134bde557c5349c0bd790343f7e6c9bc2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2237https://doaj.org/toc/2076-2607Infections caused by non-tuberculous mycobacteria (NTM) have been a public health problem in recent decades and contribute significantly to the clinical and economic burden globally. The diagnosis of infections is difficult and time-consuming and, in addition, the conventional diagnostics tests do not have sufficient discrimination power in species identification due to cross-reactions and not fully specific probes. However, technological advances have been made and the whole genome sequencing (WGS) method has been shown to be an essential part of routine diagnostics in clinical mycobacteriology laboratories. The use of this technology has contributed to the characterization of new species of mycobacteria, as well as the identification of gene mutations encoding resistance and virulence factors. Sequencing data also allowed to track global outbreaks of nosocomial NTM infections caused by <i>M. abscessus</i> complex and <i>M. chimaera</i>. To highlight the utility of WGS, we summarize recent scientific studies on WGS as a tool suitable for the management of NTM-induced infections in clinical practice.Matúš DohálIgor PorvazníkIvan SolovičJuraj MokrýMDPI AGarticlewhole-genome sequencingnon-tuberculous mycobacteriadiagnosticsBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2237, p 2237 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	whole-genome sequencing non-tuberculous mycobacteria diagnostics Biology (General) QH301-705.5
spellingShingle	whole-genome sequencing non-tuberculous mycobacteria diagnostics Biology (General) QH301-705.5 Matúš Dohál Igor Porvazník Ivan Solovič Juraj Mokrý Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections
description	Infections caused by non-tuberculous mycobacteria (NTM) have been a public health problem in recent decades and contribute significantly to the clinical and economic burden globally. The diagnosis of infections is difficult and time-consuming and, in addition, the conventional diagnostics tests do not have sufficient discrimination power in species identification due to cross-reactions and not fully specific probes. However, technological advances have been made and the whole genome sequencing (WGS) method has been shown to be an essential part of routine diagnostics in clinical mycobacteriology laboratories. The use of this technology has contributed to the characterization of new species of mycobacteria, as well as the identification of gene mutations encoding resistance and virulence factors. Sequencing data also allowed to track global outbreaks of nosocomial NTM infections caused by <i>M. abscessus</i> complex and <i>M. chimaera</i>. To highlight the utility of WGS, we summarize recent scientific studies on WGS as a tool suitable for the management of NTM-induced infections in clinical practice.
format	article
author	Matúš Dohál Igor Porvazník Ivan Solovič Juraj Mokrý
author_facet	Matúš Dohál Igor Porvazník Ivan Solovič Juraj Mokrý
author_sort	Matúš Dohál
title	Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections
title_short	Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections
title_full	Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections
title_fullStr	Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections
title_full_unstemmed	Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections
title_sort	whole genome sequencing in the management of non-tuberculous mycobacterial infections
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/134bde557c5349c0bd790343f7e6c9bc
work_keys_str_mv	AT matusdohal wholegenomesequencinginthemanagementofnontuberculousmycobacterialinfections AT igorporvaznik wholegenomesequencinginthemanagementofnontuberculousmycobacterialinfections AT ivansolovic wholegenomesequencinginthemanagementofnontuberculousmycobacterialinfections AT jurajmokry wholegenomesequencinginthemanagementofnontuberculousmycobacterialinfections
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Whole Genome Sequencing in the Management of Non-Tuberculous Mycobacterial Infections

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