CTAG-containing cleavage site profiling to delineate Salmonella into natural clusters.

<h4>Background</h4>The bacterial genus Salmonella contains thousands of serotypes that infect humans or other hosts, causing mild gastroenteritis to potentially fatal systemic infections in humans. Pathogenically distinct Salmonella serotypes have been classified as individual species or...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Le Tang, Wei-Qiao Liu, Xin Fang, Qiang Sun, Song-Ling Zhu, Chun-Xiao Wang, Xiao-Yu Wang, Yong-Guo Li, Da-Ling Zhu, Kenneth E Sanderson, Randal N Johnston, Gui-Rong Liu, Shu-Lin Liu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/134caea9327a4a2a8d71005806a3f87a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:134caea9327a4a2a8d71005806a3f87a
record_format	dspace
spelling	oai:doaj.org-article:134caea9327a4a2a8d71005806a3f87a2021-11-25T06:04:06ZCTAG-containing cleavage site profiling to delineate Salmonella into natural clusters.1932-620310.1371/journal.pone.0103388https://doaj.org/article/134caea9327a4a2a8d71005806a3f87a2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25137186/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The bacterial genus Salmonella contains thousands of serotypes that infect humans or other hosts, causing mild gastroenteritis to potentially fatal systemic infections in humans. Pathogenically distinct Salmonella serotypes have been classified as individual species or as serological variants of merely one or two species, causing considerable confusion in both research and clinical settings. This situation reflects a long unanswered question regarding whether the Salmonella serotypes exist as discrete genetic clusters (natural species) of organisms or as phenotypic (e.g. pathogenic) variants of a single (or two) natural species with a continuous spectrum of genetic divergence among them. Our recent work, based on genomic sequence divergence analysis, has demonstrated that genetic boundaries exist among Salmonella serotypes, circumscribing them into clear-cut genetic clusters of bacteria.<h4>Methodologies/principal findings</h4>To further test the genetic boundary concept for delineating Salmonella into clearly defined natural lineages (e.g., species), we sampled a small subset of conserved genomic DNA sequences, i.e., the endonuclease cleavage sites that contain the highly conserved CTAG sequence such as TCTAGA for XbaI. We found that the CTAG-containing cleavage sequence profiles could be used to resolve the genetic boundaries as reliably and efficiently as whole genome sequence comparisons but with enormously reduced requirements for time and resources.<h4>Conclusions</h4>Profiling of CTAG sequence subsets reflects genetic boundaries among Salmonella lineages and can delineate these bacteria into discrete natural clusters.Le TangWei-Qiao LiuXin FangQiang SunSong-Ling ZhuChun-Xiao WangXiao-Yu WangYong-Guo LiDa-Ling ZhuKenneth E SandersonRandal N JohnstonGui-Rong LiuShu-Lin LiuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e103388 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Le Tang Wei-Qiao Liu Xin Fang Qiang Sun Song-Ling Zhu Chun-Xiao Wang Xiao-Yu Wang Yong-Guo Li Da-Ling Zhu Kenneth E Sanderson Randal N Johnston Gui-Rong Liu Shu-Lin Liu CTAG-containing cleavage site profiling to delineate Salmonella into natural clusters.
description	<h4>Background</h4>The bacterial genus Salmonella contains thousands of serotypes that infect humans or other hosts, causing mild gastroenteritis to potentially fatal systemic infections in humans. Pathogenically distinct Salmonella serotypes have been classified as individual species or as serological variants of merely one or two species, causing considerable confusion in both research and clinical settings. This situation reflects a long unanswered question regarding whether the Salmonella serotypes exist as discrete genetic clusters (natural species) of organisms or as phenotypic (e.g. pathogenic) variants of a single (or two) natural species with a continuous spectrum of genetic divergence among them. Our recent work, based on genomic sequence divergence analysis, has demonstrated that genetic boundaries exist among Salmonella serotypes, circumscribing them into clear-cut genetic clusters of bacteria.<h4>Methodologies/principal findings</h4>To further test the genetic boundary concept for delineating Salmonella into clearly defined natural lineages (e.g., species), we sampled a small subset of conserved genomic DNA sequences, i.e., the endonuclease cleavage sites that contain the highly conserved CTAG sequence such as TCTAGA for XbaI. We found that the CTAG-containing cleavage sequence profiles could be used to resolve the genetic boundaries as reliably and efficiently as whole genome sequence comparisons but with enormously reduced requirements for time and resources.<h4>Conclusions</h4>Profiling of CTAG sequence subsets reflects genetic boundaries among Salmonella lineages and can delineate these bacteria into discrete natural clusters.
format	article
author	Le Tang Wei-Qiao Liu Xin Fang Qiang Sun Song-Ling Zhu Chun-Xiao Wang Xiao-Yu Wang Yong-Guo Li Da-Ling Zhu Kenneth E Sanderson Randal N Johnston Gui-Rong Liu Shu-Lin Liu
author_facet	Le Tang Wei-Qiao Liu Xin Fang Qiang Sun Song-Ling Zhu Chun-Xiao Wang Xiao-Yu Wang Yong-Guo Li Da-Ling Zhu Kenneth E Sanderson Randal N Johnston Gui-Rong Liu Shu-Lin Liu
author_sort	Le Tang
title	CTAG-containing cleavage site profiling to delineate Salmonella into natural clusters.
title_short	CTAG-containing cleavage site profiling to delineate Salmonella into natural clusters.
title_full	CTAG-containing cleavage site profiling to delineate Salmonella into natural clusters.
title_fullStr	CTAG-containing cleavage site profiling to delineate Salmonella into natural clusters.
title_full_unstemmed	CTAG-containing cleavage site profiling to delineate Salmonella into natural clusters.
title_sort	ctag-containing cleavage site profiling to delineate salmonella into natural clusters.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/134caea9327a4a2a8d71005806a3f87a
work_keys_str_mv	AT letang ctagcontainingcleavagesiteprofilingtodelineatesalmonellaintonaturalclusters AT weiqiaoliu ctagcontainingcleavagesiteprofilingtodelineatesalmonellaintonaturalclusters AT xinfang ctagcontainingcleavagesiteprofilingtodelineatesalmonellaintonaturalclusters AT qiangsun ctagcontainingcleavagesiteprofilingtodelineatesalmonellaintonaturalclusters AT songlingzhu ctagcontainingcleavagesiteprofilingtodelineatesalmonellaintonaturalclusters AT chunxiaowang ctagcontainingcleavagesiteprofilingtodelineatesalmonellaintonaturalclusters AT xiaoyuwang ctagcontainingcleavagesiteprofilingtodelineatesalmonellaintonaturalclusters AT yongguoli ctagcontainingcleavagesiteprofilingtodelineatesalmonellaintonaturalclusters AT dalingzhu ctagcontainingcleavagesiteprofilingtodelineatesalmonellaintonaturalclusters AT kennethesanderson ctagcontainingcleavagesiteprofilingtodelineatesalmonellaintonaturalclusters AT randalnjohnston ctagcontainingcleavagesiteprofilingtodelineatesalmonellaintonaturalclusters AT guirongliu ctagcontainingcleavagesiteprofilingtodelineatesalmonellaintonaturalclusters AT shulinliu ctagcontainingcleavagesiteprofilingtodelineatesalmonellaintonaturalclusters
_version_	1718414206671257600

CTAG-containing cleavage site profiling to delineate Salmonella into natural clusters.

Ejemplares similares