Twitching motility of Stenotrophomonas maltophilia under iron limitation: In-silico, phenotypic and proteomic approaches

This study investigates the twitching ability of 28 clinical and five environmental strains of S. maltophilia grown under iron-depleted condition through in-silico, phenotypic and proteomics approaches. Rapid Annotations using Subsystem Technology (RAST) analysis revealed the presence of 21 targets...

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Autores principales:	V. Kalidasan, Vasantha Kumari Neela
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	stenotrophomonas maltophilia iron depletion rast server twitching motility itraq type iv pili Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/1350a89229a94099988603966cf888a6
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spelling	oai:doaj.org-article:1350a89229a94099988603966cf888a62021-11-17T14:21:57ZTwitching motility of Stenotrophomonas maltophilia under iron limitation: In-silico, phenotypic and proteomic approaches2150-55942150-560810.1080/21505594.2020.1713649https://doaj.org/article/1350a89229a94099988603966cf888a62020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1713649https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608This study investigates the twitching ability of 28 clinical and five environmental strains of S. maltophilia grown under iron-depleted condition through in-silico, phenotypic and proteomics approaches. Rapid Annotations using Subsystem Technology (RAST) analysis revealed the presence of 21 targets of type IV pilus shared across S. maltophilia strains K279a, R551-3, D457 and JV3. The macroscopic twitching assay showed that only clinical isolates produced a zone of twitching with a mean of 22.00 mm under normal and 25.00 mm under iron-depleted conditions. (p = 0.002). Environmental isolates did not show any significant twitching activity in both conditions tested. Isobaric Tags for Relative and Absolute Quantification (ITRAQ) analysis showed altered expression of twitching motility protein PilT (99.08-fold change), flagellar biosynthesis protein FliC (20.14-fold change), and fimbrial protein (0.70-fold change) in response to iron-depleted condition. Most of the strains that have the ability to twitch under the normal condition, exhibit enhanced twitching during iron limitation.V. KalidasanVasantha Kumari NeelaTaylor & Francis Grouparticlestenotrophomonas maltophiliairon depletionrast servertwitching motilityitraqtype iv piliInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 104-112 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	stenotrophomonas maltophilia iron depletion rast server twitching motility itraq type iv pili Infectious and parasitic diseases RC109-216
spellingShingle	stenotrophomonas maltophilia iron depletion rast server twitching motility itraq type iv pili Infectious and parasitic diseases RC109-216 V. Kalidasan Vasantha Kumari Neela Twitching motility of Stenotrophomonas maltophilia under iron limitation: In-silico, phenotypic and proteomic approaches
description	This study investigates the twitching ability of 28 clinical and five environmental strains of S. maltophilia grown under iron-depleted condition through in-silico, phenotypic and proteomics approaches. Rapid Annotations using Subsystem Technology (RAST) analysis revealed the presence of 21 targets of type IV pilus shared across S. maltophilia strains K279a, R551-3, D457 and JV3. The macroscopic twitching assay showed that only clinical isolates produced a zone of twitching with a mean of 22.00 mm under normal and 25.00 mm under iron-depleted conditions. (p = 0.002). Environmental isolates did not show any significant twitching activity in both conditions tested. Isobaric Tags for Relative and Absolute Quantification (ITRAQ) analysis showed altered expression of twitching motility protein PilT (99.08-fold change), flagellar biosynthesis protein FliC (20.14-fold change), and fimbrial protein (0.70-fold change) in response to iron-depleted condition. Most of the strains that have the ability to twitch under the normal condition, exhibit enhanced twitching during iron limitation.
format	article
author	V. Kalidasan Vasantha Kumari Neela
author_facet	V. Kalidasan Vasantha Kumari Neela
author_sort	V. Kalidasan
title	Twitching motility of Stenotrophomonas maltophilia under iron limitation: In-silico, phenotypic and proteomic approaches
title_short	Twitching motility of Stenotrophomonas maltophilia under iron limitation: In-silico, phenotypic and proteomic approaches
title_full	Twitching motility of Stenotrophomonas maltophilia under iron limitation: In-silico, phenotypic and proteomic approaches
title_fullStr	Twitching motility of Stenotrophomonas maltophilia under iron limitation: In-silico, phenotypic and proteomic approaches
title_full_unstemmed	Twitching motility of Stenotrophomonas maltophilia under iron limitation: In-silico, phenotypic and proteomic approaches
title_sort	twitching motility of stenotrophomonas maltophilia under iron limitation: in-silico, phenotypic and proteomic approaches
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/1350a89229a94099988603966cf888a6
work_keys_str_mv	AT vkalidasan twitchingmotilityofstenotrophomonasmaltophiliaunderironlimitationinsilicophenotypicandproteomicapproaches AT vasanthakumarineela twitchingmotilityofstenotrophomonasmaltophiliaunderironlimitationinsilicophenotypicandproteomicapproaches
_version_	1718425464120279040

Twitching motility of Stenotrophomonas maltophilia under iron limitation: In-silico, phenotypic and proteomic approaches

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