Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples

Abstract Leprosy, a progressive, mutilating and highly stigmatized disease caused by Mycobacterium leprae (ML), continues to prevail in the developing world. This is due to the absence of rapid, specific and sensitive diagnostic tools for its early detection since the disease gets notified only with...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nupur Garg, Upasana Sahu, Sudeshna Kar, Farhan J. Ahmad
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1359fb8d7fa745a7afdf401602c83082
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:1359fb8d7fa745a7afdf401602c83082
record_format	dspace
spelling	oai:doaj.org-article:1359fb8d7fa745a7afdf401602c830822021-12-02T15:36:14ZDevelopment of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples10.1038/s41598-021-89304-22045-2322https://doaj.org/article/1359fb8d7fa745a7afdf401602c830822021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89304-2https://doaj.org/toc/2045-2322Abstract Leprosy, a progressive, mutilating and highly stigmatized disease caused by Mycobacterium leprae (ML), continues to prevail in the developing world. This is due to the absence of rapid, specific and sensitive diagnostic tools for its early detection since the disease gets notified only with the advent of physical scarring in patients. This study reports the development of a Loop-mediated isothermal amplification (LAMP) technique for fast, sensitive and specific amplification of 16S rRNA gene of ML DNA for early detection of leprosy in resource-limited areas. Various parameters were optimized to obtain robust and reliable amplification of ML DNA. Blind clinical validation studies were performed which showed that this technique had complete concurrence with conventional techniques. Total absence of amplification of negative control DNA confirmed the specificity of this test. Various visual detection methods viz. colorimetric, turbidity differentiation and bridge flocculation were standardized to establish easy-to-read and rapid diagnosis. This technique eliminates the lack of accuracy and sensitivity in skin smear tests in patients and the requirement for expensive lab equipments and trained technicians. The technique holds promise for further expansion and has the potential to cater to the unmet needs of society for a cheap, highly-sensitive and robust rapid diagnosis of ML.Nupur GargUpasana SahuSudeshna KarFarhan J. AhmadNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nupur Garg Upasana Sahu Sudeshna Kar Farhan J. Ahmad Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples
description	Abstract Leprosy, a progressive, mutilating and highly stigmatized disease caused by Mycobacterium leprae (ML), continues to prevail in the developing world. This is due to the absence of rapid, specific and sensitive diagnostic tools for its early detection since the disease gets notified only with the advent of physical scarring in patients. This study reports the development of a Loop-mediated isothermal amplification (LAMP) technique for fast, sensitive and specific amplification of 16S rRNA gene of ML DNA for early detection of leprosy in resource-limited areas. Various parameters were optimized to obtain robust and reliable amplification of ML DNA. Blind clinical validation studies were performed which showed that this technique had complete concurrence with conventional techniques. Total absence of amplification of negative control DNA confirmed the specificity of this test. Various visual detection methods viz. colorimetric, turbidity differentiation and bridge flocculation were standardized to establish easy-to-read and rapid diagnosis. This technique eliminates the lack of accuracy and sensitivity in skin smear tests in patients and the requirement for expensive lab equipments and trained technicians. The technique holds promise for further expansion and has the potential to cater to the unmet needs of society for a cheap, highly-sensitive and robust rapid diagnosis of ML.
format	article
author	Nupur Garg Upasana Sahu Sudeshna Kar Farhan J. Ahmad
author_facet	Nupur Garg Upasana Sahu Sudeshna Kar Farhan J. Ahmad
author_sort	Nupur Garg
title	Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples
title_short	Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples
title_full	Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples
title_fullStr	Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples
title_full_unstemmed	Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples
title_sort	development of a loop-mediated isothermal amplification (lamp) technique for specific and early detection of mycobacterium leprae in clinical samples
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1359fb8d7fa745a7afdf401602c83082
work_keys_str_mv	AT nupurgarg developmentofaloopmediatedisothermalamplificationlamptechniqueforspecificandearlydetectionofmycobacteriumlepraeinclinicalsamples AT upasanasahu developmentofaloopmediatedisothermalamplificationlamptechniqueforspecificandearlydetectionofmycobacteriumlepraeinclinicalsamples AT sudeshnakar developmentofaloopmediatedisothermalamplificationlamptechniqueforspecificandearlydetectionofmycobacteriumlepraeinclinicalsamples AT farhanjahmad developmentofaloopmediatedisothermalamplificationlamptechniqueforspecificandearlydetectionofmycobacteriumlepraeinclinicalsamples
_version_	1718386336909492224

Development of a Loop-mediated isothermal amplification (LAMP) technique for specific and early detection of Mycobacterium leprae in clinical samples

Ejemplares similares