Effect of mupirocin for Staphylococcus aureus decolonization on the microbiome of the nose and throat in community and nursing home dwelling adults.

<h4>Objective</h4>To characterize the microbial communities of the anterior nares (nose) and posterior pharynx (throat) of adults dwelling in the community and in nursing homes before and after treatment with intranasal mupirocin.<h4>Methods</h4>Staphylococcus aureus-colonize...

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Autores principales:	Mary-Claire Roghmann, Alison D Lydecker, Michelle Shardell, Robert T DeBoy, J Kristie Johnson, LiCheng Zhao, Lauren L Hittle, Emmanuel F Mongodin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/135c167ab01b400a9f5e5a407f797709
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spelling	oai:doaj.org-article:135c167ab01b400a9f5e5a407f7977092021-12-02T20:03:54ZEffect of mupirocin for Staphylococcus aureus decolonization on the microbiome of the nose and throat in community and nursing home dwelling adults.1932-620310.1371/journal.pone.0252004https://doaj.org/article/135c167ab01b400a9f5e5a407f7977092021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252004https://doaj.org/toc/1932-6203<h4>Objective</h4>To characterize the microbial communities of the anterior nares (nose) and posterior pharynx (throat) of adults dwelling in the community and in nursing homes before and after treatment with intranasal mupirocin.<h4>Methods</h4>Staphylococcus aureus-colonized adults were recruited from the community (n = 25) and from nursing homes (n = 7). S. aureus colonization was confirmed using cultures. Participants had specimens taken from nose and throat for S. aureus quantitation using quantitative PCR for the nuc gene and bacterial profiling using 16S rRNA gene sequencing over 12 weeks. After two baseline study visits 4 weeks apart, participants received intranasal mupirocin for 5 days with 3 further visits over a 8 week follow-up period.<h4>Results</h4>We found a decrease in the absolute abundance of S. aureus in the nose for 8 weeks after mupirocin (1693 vs 141 fg/ul, p = 0.047). Mupirocin caused a statistically significant disruption in bacterial communities of the nose and throat after 1 week, which was no longer detected after 8 weeks. Bacterial community profiling demonstrated that there was a decrease in the relative abundance of S. aureus (8% vs 0.3%, p<0.01) 8 weeks after mupirocin and a transient decrease in the relative abundance of Staphylococcus epidermidis in the nose (21% vs 5%, p<0.01) 1 week after mupirocin.<h4>Conclusions</h4>Decolonization with mupirocin leads to a sustained effect on absolute and relative abundance of S. aureus but not for other bacteria in the nose. This demonstrates that a short course of mupirocin selectively decreases S. aureus in the nose for up to 8 weeks.Mary-Claire RoghmannAlison D LydeckerMichelle ShardellRobert T DeBoyJ Kristie JohnsonLiCheng ZhaoLauren L HittleEmmanuel F MongodinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252004 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mary-Claire Roghmann Alison D Lydecker Michelle Shardell Robert T DeBoy J Kristie Johnson LiCheng Zhao Lauren L Hittle Emmanuel F Mongodin Effect of mupirocin for Staphylococcus aureus decolonization on the microbiome of the nose and throat in community and nursing home dwelling adults.
description	<h4>Objective</h4>To characterize the microbial communities of the anterior nares (nose) and posterior pharynx (throat) of adults dwelling in the community and in nursing homes before and after treatment with intranasal mupirocin.<h4>Methods</h4>Staphylococcus aureus-colonized adults were recruited from the community (n = 25) and from nursing homes (n = 7). S. aureus colonization was confirmed using cultures. Participants had specimens taken from nose and throat for S. aureus quantitation using quantitative PCR for the nuc gene and bacterial profiling using 16S rRNA gene sequencing over 12 weeks. After two baseline study visits 4 weeks apart, participants received intranasal mupirocin for 5 days with 3 further visits over a 8 week follow-up period.<h4>Results</h4>We found a decrease in the absolute abundance of S. aureus in the nose for 8 weeks after mupirocin (1693 vs 141 fg/ul, p = 0.047). Mupirocin caused a statistically significant disruption in bacterial communities of the nose and throat after 1 week, which was no longer detected after 8 weeks. Bacterial community profiling demonstrated that there was a decrease in the relative abundance of S. aureus (8% vs 0.3%, p<0.01) 8 weeks after mupirocin and a transient decrease in the relative abundance of Staphylococcus epidermidis in the nose (21% vs 5%, p<0.01) 1 week after mupirocin.<h4>Conclusions</h4>Decolonization with mupirocin leads to a sustained effect on absolute and relative abundance of S. aureus but not for other bacteria in the nose. This demonstrates that a short course of mupirocin selectively decreases S. aureus in the nose for up to 8 weeks.
format	article
author	Mary-Claire Roghmann Alison D Lydecker Michelle Shardell Robert T DeBoy J Kristie Johnson LiCheng Zhao Lauren L Hittle Emmanuel F Mongodin
author_facet	Mary-Claire Roghmann Alison D Lydecker Michelle Shardell Robert T DeBoy J Kristie Johnson LiCheng Zhao Lauren L Hittle Emmanuel F Mongodin
author_sort	Mary-Claire Roghmann
title	Effect of mupirocin for Staphylococcus aureus decolonization on the microbiome of the nose and throat in community and nursing home dwelling adults.
title_short	Effect of mupirocin for Staphylococcus aureus decolonization on the microbiome of the nose and throat in community and nursing home dwelling adults.
title_full	Effect of mupirocin for Staphylococcus aureus decolonization on the microbiome of the nose and throat in community and nursing home dwelling adults.
title_fullStr	Effect of mupirocin for Staphylococcus aureus decolonization on the microbiome of the nose and throat in community and nursing home dwelling adults.
title_full_unstemmed	Effect of mupirocin for Staphylococcus aureus decolonization on the microbiome of the nose and throat in community and nursing home dwelling adults.
title_sort	effect of mupirocin for staphylococcus aureus decolonization on the microbiome of the nose and throat in community and nursing home dwelling adults.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/135c167ab01b400a9f5e5a407f797709
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Effect of mupirocin for Staphylococcus aureus decolonization on the microbiome of the nose and throat in community and nursing home dwelling adults.

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