Morphofunctional, viability and antioxidant system alterations on rat primary testicular cells exposed to simulated microgravity

This study focused on effects induced by short-term simulated microgravity (SMG) condition on primary cell culture from pre-pubertal Wistar rats testis. Cells were analyzed for cytoskeletal and Sex Hormone Binding Globulin (SHBG/ABP) changes by immunofluorescence technique, for antioxidant system e...

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Autores principales:	Valentina Bonetto, Linda Scarabelli, Maria Angela Masini
Formato:	article
Lenguaje:	EN
Publicado:	PAGEPress Publications 2021
Materias:	Testicular primary culture simulated microgravity cytoskeleton oxidative stress Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/1367ca9f8e844402adeea05649434906
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spelling	oai:doaj.org-article:1367ca9f8e844402adeea056494349062021-11-12T21:38:58ZMorphofunctional, viability and antioxidant system alterations on rat primary testicular cells exposed to simulated microgravity10.4081/jbr.2021.98751826-88382284-0230https://doaj.org/article/1367ca9f8e844402adeea056494349062021-11-01T00:00:00Zhttps://www.pagepressjournals.org/index.php/jbr/article/view/9875https://doaj.org/toc/1826-8838https://doaj.org/toc/2284-0230 This study focused on effects induced by short-term simulated microgravity (SMG) condition on primary cell culture from pre-pubertal Wistar rats testis. Cells were analyzed for cytoskeletal and Sex Hormone Binding Globulin (SHBG/ABP) changes by immunofluorescence technique, for antioxidant system exploiting RT-PCR and cell viability. Cells were cultured for 6 and 24h on a three-dimensional clinostat, Random Positioning Machine (RPM). At the end of each experiment, once stopped the RPM rotation, cells were either fixed in paraformaldehyde or lysed and RNA extracted. In cells exposed to SMG the cytoskeleton became disorganized, microtubules fragmented and SHBG was already undetectable after 6h of treatment. Moreover, various antioxidant systems significantly increased after 24h of SMG exposure. Initially, SMG seemed to disturb antioxidant protection strategies allowing the testes to support sperm production, thus generating an aging-like state of oxidative stress. Studies on changes induced by short-term altered gravity conditions, carried out in real microgravity, could give more information on steroidogenesis and germ cell differentiation within the testis exposed to this condition and confirm the validity of simulation approach. Valentina BonettoLinda ScarabelliMaria Angela MasiniPAGEPress PublicationsarticleTesticular primary culturesimulated microgravitycytoskeletonoxidative stressBiology (General)QH301-705.5ENJournal of Biological Research (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Testicular primary culture simulated microgravity cytoskeleton oxidative stress Biology (General) QH301-705.5
spellingShingle	Testicular primary culture simulated microgravity cytoskeleton oxidative stress Biology (General) QH301-705.5 Valentina Bonetto Linda Scarabelli Maria Angela Masini Morphofunctional, viability and antioxidant system alterations on rat primary testicular cells exposed to simulated microgravity
description	This study focused on effects induced by short-term simulated microgravity (SMG) condition on primary cell culture from pre-pubertal Wistar rats testis. Cells were analyzed for cytoskeletal and Sex Hormone Binding Globulin (SHBG/ABP) changes by immunofluorescence technique, for antioxidant system exploiting RT-PCR and cell viability. Cells were cultured for 6 and 24h on a three-dimensional clinostat, Random Positioning Machine (RPM). At the end of each experiment, once stopped the RPM rotation, cells were either fixed in paraformaldehyde or lysed and RNA extracted. In cells exposed to SMG the cytoskeleton became disorganized, microtubules fragmented and SHBG was already undetectable after 6h of treatment. Moreover, various antioxidant systems significantly increased after 24h of SMG exposure. Initially, SMG seemed to disturb antioxidant protection strategies allowing the testes to support sperm production, thus generating an aging-like state of oxidative stress. Studies on changes induced by short-term altered gravity conditions, carried out in real microgravity, could give more information on steroidogenesis and germ cell differentiation within the testis exposed to this condition and confirm the validity of simulation approach.
format	article
author	Valentina Bonetto Linda Scarabelli Maria Angela Masini
author_facet	Valentina Bonetto Linda Scarabelli Maria Angela Masini
author_sort	Valentina Bonetto
title	Morphofunctional, viability and antioxidant system alterations on rat primary testicular cells exposed to simulated microgravity
title_short	Morphofunctional, viability and antioxidant system alterations on rat primary testicular cells exposed to simulated microgravity
title_full	Morphofunctional, viability and antioxidant system alterations on rat primary testicular cells exposed to simulated microgravity
title_fullStr	Morphofunctional, viability and antioxidant system alterations on rat primary testicular cells exposed to simulated microgravity
title_full_unstemmed	Morphofunctional, viability and antioxidant system alterations on rat primary testicular cells exposed to simulated microgravity
title_sort	morphofunctional, viability and antioxidant system alterations on rat primary testicular cells exposed to simulated microgravity
publisher	PAGEPress Publications
publishDate	2021
url	https://doaj.org/article/1367ca9f8e844402adeea05649434906
work_keys_str_mv	AT valentinabonetto morphofunctionalviabilityandantioxidantsystemalterationsonratprimarytesticularcellsexposedtosimulatedmicrogravity AT lindascarabelli morphofunctionalviabilityandantioxidantsystemalterationsonratprimarytesticularcellsexposedtosimulatedmicrogravity AT mariaangelamasini morphofunctionalviabilityandantioxidantsystemalterationsonratprimarytesticularcellsexposedtosimulatedmicrogravity
_version_	1718430298505478144

Morphofunctional, viability and antioxidant system alterations on rat primary testicular cells exposed to simulated microgravity

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