The Evolution of Fluoroquinolone Resistance in <i>Salmonella</i> under Exposure to Sub-Inhibitory Concentration of Enrofloxacin
The evolution of resistance in <i>Salmonella</i> to fluoroquinolones (FQs) under a broad range of sub-inhibitory concentrations (sub-MICs) has not been systematically studied. This study investigated the mechanism of resistance development in <i>Salmonella enterica</i> serova...
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/1369a979c115480986ff6e6d73eda44c |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:1369a979c115480986ff6e6d73eda44c |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:1369a979c115480986ff6e6d73eda44c2021-11-25T17:54:25ZThe Evolution of Fluoroquinolone Resistance in <i>Salmonella</i> under Exposure to Sub-Inhibitory Concentration of Enrofloxacin10.3390/ijms2222122181422-00671661-6596https://doaj.org/article/1369a979c115480986ff6e6d73eda44c2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12218https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The evolution of resistance in <i>Salmonella</i> to fluoroquinolones (FQs) under a broad range of sub-inhibitory concentrations (sub-MICs) has not been systematically studied. This study investigated the mechanism of resistance development in <i>Salmonella enterica</i> serovar Enteritidis (<i>S</i>. Enteritidis) under sub-MICs of 1/128×MIC to 1/2×MIC of enrofloxacin (ENR), a widely used veterinary FQ. It was shown that the resistance rate and resistance level of <i>S</i>. Enteritidis varied with the increase in ENR concentration and duration of selection. qRT-PCR results demonstrated that the expression of outer membrane porin (OMP) genes, <i>omp</i><i>C</i>, <i>omp</i><i>D</i> and <i>omp</i><i>F</i>, were down-regulated first to rapidly adapt and develop the resistance of 4×MIC, and as the resistance level increased (≥8×MIC), the up-regulated expression of efflux pump genes, <i>acrB</i>, <i>emrB</i> amd <i>mdfA</i>, along with mutations in quinolone resistance-determining region (QRDR) gradually played a decisive role. Cytohubba analysis based on transcriptomic profiles demonstrated that <i>purB</i>, <i>purC</i>, <i>purD</i>, <i>purF</i>, <i>purH</i>, <i>purK</i>, <i>purL</i>, <i>purM</i>, <i>purN</i> and <i>purT</i> were the hub genes for the FQs resistance. The ‘de novo’ IMP biosynthetic process, purine ribonucleoside monophosphate biosynthetic process and purine ribonucleotide biosynthetic process were the top three biological processes screened by MCODE. This study first described the dynamics of FQ resistance evolution in <i>Salmonella</i> under a long-term selection of sub-MICs of ENR in vitro. In addition, this work offers greater insight into the transcriptome changes of <i>S</i>. Enteritidis under the selection of ENR and provides a framework for FQs resistance of <i>Salmonella</i> for further studies.Yufeng GuLulu HuangCuirong WuJunhong HuangHaihong HaoZonghui YuanGuyue ChengMDPI AGarticle<i>Salmonella</i>enrofloxacinresistancesub-inhibitory concentrationtranscriptome sequencingBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12218, p 12218 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
<i>Salmonella</i> enrofloxacin resistance sub-inhibitory concentration transcriptome sequencing Biology (General) QH301-705.5 Chemistry QD1-999 |
| spellingShingle |
<i>Salmonella</i> enrofloxacin resistance sub-inhibitory concentration transcriptome sequencing Biology (General) QH301-705.5 Chemistry QD1-999 Yufeng Gu Lulu Huang Cuirong Wu Junhong Huang Haihong Hao Zonghui Yuan Guyue Cheng The Evolution of Fluoroquinolone Resistance in <i>Salmonella</i> under Exposure to Sub-Inhibitory Concentration of Enrofloxacin |
| description |
The evolution of resistance in <i>Salmonella</i> to fluoroquinolones (FQs) under a broad range of sub-inhibitory concentrations (sub-MICs) has not been systematically studied. This study investigated the mechanism of resistance development in <i>Salmonella enterica</i> serovar Enteritidis (<i>S</i>. Enteritidis) under sub-MICs of 1/128×MIC to 1/2×MIC of enrofloxacin (ENR), a widely used veterinary FQ. It was shown that the resistance rate and resistance level of <i>S</i>. Enteritidis varied with the increase in ENR concentration and duration of selection. qRT-PCR results demonstrated that the expression of outer membrane porin (OMP) genes, <i>omp</i><i>C</i>, <i>omp</i><i>D</i> and <i>omp</i><i>F</i>, were down-regulated first to rapidly adapt and develop the resistance of 4×MIC, and as the resistance level increased (≥8×MIC), the up-regulated expression of efflux pump genes, <i>acrB</i>, <i>emrB</i> amd <i>mdfA</i>, along with mutations in quinolone resistance-determining region (QRDR) gradually played a decisive role. Cytohubba analysis based on transcriptomic profiles demonstrated that <i>purB</i>, <i>purC</i>, <i>purD</i>, <i>purF</i>, <i>purH</i>, <i>purK</i>, <i>purL</i>, <i>purM</i>, <i>purN</i> and <i>purT</i> were the hub genes for the FQs resistance. The ‘de novo’ IMP biosynthetic process, purine ribonucleoside monophosphate biosynthetic process and purine ribonucleotide biosynthetic process were the top three biological processes screened by MCODE. This study first described the dynamics of FQ resistance evolution in <i>Salmonella</i> under a long-term selection of sub-MICs of ENR in vitro. In addition, this work offers greater insight into the transcriptome changes of <i>S</i>. Enteritidis under the selection of ENR and provides a framework for FQs resistance of <i>Salmonella</i> for further studies. |
| format |
article |
| author |
Yufeng Gu Lulu Huang Cuirong Wu Junhong Huang Haihong Hao Zonghui Yuan Guyue Cheng |
| author_facet |
Yufeng Gu Lulu Huang Cuirong Wu Junhong Huang Haihong Hao Zonghui Yuan Guyue Cheng |
| author_sort |
Yufeng Gu |
| title |
The Evolution of Fluoroquinolone Resistance in <i>Salmonella</i> under Exposure to Sub-Inhibitory Concentration of Enrofloxacin |
| title_short |
The Evolution of Fluoroquinolone Resistance in <i>Salmonella</i> under Exposure to Sub-Inhibitory Concentration of Enrofloxacin |
| title_full |
The Evolution of Fluoroquinolone Resistance in <i>Salmonella</i> under Exposure to Sub-Inhibitory Concentration of Enrofloxacin |
| title_fullStr |
The Evolution of Fluoroquinolone Resistance in <i>Salmonella</i> under Exposure to Sub-Inhibitory Concentration of Enrofloxacin |
| title_full_unstemmed |
The Evolution of Fluoroquinolone Resistance in <i>Salmonella</i> under Exposure to Sub-Inhibitory Concentration of Enrofloxacin |
| title_sort |
evolution of fluoroquinolone resistance in <i>salmonella</i> under exposure to sub-inhibitory concentration of enrofloxacin |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/1369a979c115480986ff6e6d73eda44c |
| work_keys_str_mv |
AT yufenggu theevolutionoffluoroquinoloneresistanceinisalmonellaiunderexposuretosubinhibitoryconcentrationofenrofloxacin AT luluhuang theevolutionoffluoroquinoloneresistanceinisalmonellaiunderexposuretosubinhibitoryconcentrationofenrofloxacin AT cuirongwu theevolutionoffluoroquinoloneresistanceinisalmonellaiunderexposuretosubinhibitoryconcentrationofenrofloxacin AT junhonghuang theevolutionoffluoroquinoloneresistanceinisalmonellaiunderexposuretosubinhibitoryconcentrationofenrofloxacin AT haihonghao theevolutionoffluoroquinoloneresistanceinisalmonellaiunderexposuretosubinhibitoryconcentrationofenrofloxacin AT zonghuiyuan theevolutionoffluoroquinoloneresistanceinisalmonellaiunderexposuretosubinhibitoryconcentrationofenrofloxacin AT guyuecheng theevolutionoffluoroquinoloneresistanceinisalmonellaiunderexposuretosubinhibitoryconcentrationofenrofloxacin AT yufenggu evolutionoffluoroquinoloneresistanceinisalmonellaiunderexposuretosubinhibitoryconcentrationofenrofloxacin AT luluhuang evolutionoffluoroquinoloneresistanceinisalmonellaiunderexposuretosubinhibitoryconcentrationofenrofloxacin AT cuirongwu evolutionoffluoroquinoloneresistanceinisalmonellaiunderexposuretosubinhibitoryconcentrationofenrofloxacin AT junhonghuang evolutionoffluoroquinoloneresistanceinisalmonellaiunderexposuretosubinhibitoryconcentrationofenrofloxacin AT haihonghao evolutionoffluoroquinoloneresistanceinisalmonellaiunderexposuretosubinhibitoryconcentrationofenrofloxacin AT zonghuiyuan evolutionoffluoroquinoloneresistanceinisalmonellaiunderexposuretosubinhibitoryconcentrationofenrofloxacin AT guyuecheng evolutionoffluoroquinoloneresistanceinisalmonellaiunderexposuretosubinhibitoryconcentrationofenrofloxacin |
| _version_ |
1718411846772326400 |