Molecular test for detection of Mycoplasma ovipneumoniae associated with respiratory tract infection from goats in north and central parts of Kerala

Mycoplasmal pneumonia is an important contagious disease that significantly affects the economy of small ruminant farming worldwide and Mycoplasma ovipneumoniae (M. ovipneumoniae) is one of the major aetiological agents associated with pleuropneumonia in goats. It is considered as a serious epidemic...

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Autores principales: P. Santhiya, Surya Sankar, M. Mini, Siju Joseph, R. Thirupathy Venkatachalapathy
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Lenguaje:EN
Publicado: Director of Academics and Research, Kerala Veterinary and Animal Sciences University 2021
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spelling oai:doaj.org-article:136b8857ac2148d5bb38446ef93cf60e2021-11-15T14:05:15ZMolecular test for detection of Mycoplasma ovipneumoniae associated with respiratory tract infection from goats in north and central parts of Kerala10.51966/jvas.2021.52.3.267-2710971-07012582-0605https://doaj.org/article/136b8857ac2148d5bb38446ef93cf60e2021-07-01T00:00:00Zhttps://www.jvas.in/public_html/upload/article_file/article_file_r0qk64.pdf?t=r0qk64https://doaj.org/toc/0971-0701https://doaj.org/toc/2582-0605Mycoplasmal pneumonia is an important contagious disease that significantly affects the economy of small ruminant farming worldwide and Mycoplasma ovipneumoniae (M. ovipneumoniae) is one of the major aetiological agents associated with pleuropneumonia in goats. It is considered as a serious epidemic disease of goats due to its huge economic impact and hence, rapid and early diagnosis of the disease is warranted. Clinical mycoplasmosis often lacks pathognomonic signs, so definitive diagnosis of the disease is quite burdensome. Polymerase chain reaction (PCR) test has been proven to be a specific and sensitive technique for the early diagnosis of mycoplasmosis. The present study highlights the detection of M. ovipneumoniae employing PCR test in 150 nasal swab samples collected from goats with symptoms of respiratory tract infection from five districts of Kerala. Results revealed that, out of 150 samples, 83 (55.33 per cent) were positive in 16S rRNA Mycoplasma genus specific PCR test. Among the 83 genus positive samples, 68 samples (45.33 per cent of total 150 samples) were positive in M. ovipneumoniae specific PCR test. P. SanthiyaSurya SankarM. MiniSiju JosephR. Thirupathy VenkatachalapathyDirector of Academics and Research, Kerala Veterinary and Animal Sciences Universityarticlegoatspolymerase chain reaction (pcr)mycoplasma ovipneumoniaeAnimal biochemistryQP501-801Science (General)Q1-390ENJournal of Veterinary and Animal Sciences, Vol 52, Iss 3, Pp 267-271 (2021)
institution DOAJ
collection DOAJ
language EN
topic goats
polymerase chain reaction (pcr)
mycoplasma ovipneumoniae
Animal biochemistry
QP501-801
Science (General)
Q1-390
spellingShingle goats
polymerase chain reaction (pcr)
mycoplasma ovipneumoniae
Animal biochemistry
QP501-801
Science (General)
Q1-390
P. Santhiya
Surya Sankar
M. Mini
Siju Joseph
R. Thirupathy Venkatachalapathy
Molecular test for detection of Mycoplasma ovipneumoniae associated with respiratory tract infection from goats in north and central parts of Kerala
description Mycoplasmal pneumonia is an important contagious disease that significantly affects the economy of small ruminant farming worldwide and Mycoplasma ovipneumoniae (M. ovipneumoniae) is one of the major aetiological agents associated with pleuropneumonia in goats. It is considered as a serious epidemic disease of goats due to its huge economic impact and hence, rapid and early diagnosis of the disease is warranted. Clinical mycoplasmosis often lacks pathognomonic signs, so definitive diagnosis of the disease is quite burdensome. Polymerase chain reaction (PCR) test has been proven to be a specific and sensitive technique for the early diagnosis of mycoplasmosis. The present study highlights the detection of M. ovipneumoniae employing PCR test in 150 nasal swab samples collected from goats with symptoms of respiratory tract infection from five districts of Kerala. Results revealed that, out of 150 samples, 83 (55.33 per cent) were positive in 16S rRNA Mycoplasma genus specific PCR test. Among the 83 genus positive samples, 68 samples (45.33 per cent of total 150 samples) were positive in M. ovipneumoniae specific PCR test.
format article
author P. Santhiya
Surya Sankar
M. Mini
Siju Joseph
R. Thirupathy Venkatachalapathy
author_facet P. Santhiya
Surya Sankar
M. Mini
Siju Joseph
R. Thirupathy Venkatachalapathy
author_sort P. Santhiya
title Molecular test for detection of Mycoplasma ovipneumoniae associated with respiratory tract infection from goats in north and central parts of Kerala
title_short Molecular test for detection of Mycoplasma ovipneumoniae associated with respiratory tract infection from goats in north and central parts of Kerala
title_full Molecular test for detection of Mycoplasma ovipneumoniae associated with respiratory tract infection from goats in north and central parts of Kerala
title_fullStr Molecular test for detection of Mycoplasma ovipneumoniae associated with respiratory tract infection from goats in north and central parts of Kerala
title_full_unstemmed Molecular test for detection of Mycoplasma ovipneumoniae associated with respiratory tract infection from goats in north and central parts of Kerala
title_sort molecular test for detection of mycoplasma ovipneumoniae associated with respiratory tract infection from goats in north and central parts of kerala
publisher Director of Academics and Research, Kerala Veterinary and Animal Sciences University
publishDate 2021
url https://doaj.org/article/136b8857ac2148d5bb38446ef93cf60e
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