MESENCHYMAL STEM CELLS WITH HIGH GROWTH RATE IN THE SUPERNATANT MEDIUM FROM RAT BONE MARROW PRIMARY CULTURE

BACKGROUND AND OBJECTIVE: In marrow primary culture for mesenchymal stem cell isolation, the cells suspended in supernatant are usually discarded at first medium exchange. The objective of this study was to culture and expand these cells, to examine their mesenchymal stem cell nature, to compare the...

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Autores principales:	MR Baghaban Eslaminejad, H Nazarian, L Taghiyar
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2008
Materias:	mesenchymal stem cells removed medium growth rate bone cartilage and adipose differentiation Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/137ca52ad17e4d958c89f91400126b97
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spelling	oai:doaj.org-article:137ca52ad17e4d958c89f91400126b972021-11-10T09:07:27ZMESENCHYMAL STEM CELLS WITH HIGH GROWTH RATE IN THE SUPERNATANT MEDIUM FROM RAT BONE MARROW PRIMARY CULTURE1561-41072251-7170https://doaj.org/article/137ca52ad17e4d958c89f91400126b972008-06-01T00:00:00Zhttp://jbums.org/article-1-2975-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: In marrow primary culture for mesenchymal stem cell isolation, the cells suspended in supernatant are usually discarded at first medium exchange. The objective of this study was to culture and expand these cells, to examine their mesenchymal stem cell nature, to compare their growth rate with that of adherent cells of primary culture and eventually to optimize the culture conditions for the cells maximum proliferation.METHODS: In this experimental study, 10 Wistar strain rats were sacrificed, their tibial bone marrow were collected and plated in 75cm2 culture flasks. Four days after culture initiation, the supernatant medium was collected and its floating cells along with those primarily adherent cells were expanded by three successive subcultures, during which population doubling time (PDT) was calculated and statistically compared. Furthermore, culture condition including its fetal bovine serum (FBS) concentration as well as the cell density at culture initiation for maximum proliferation of the cells was optimized. The isolated cells were evaluated with respect to their potential to differentiate into bone, cartilage and adipose cells. FINDINGS: Based on our results, the population of the cells from removed medium was doubled in number each 14.9±2 hr, while this value was 19±2.1 hr for primarily adherent cell population (p<0.05). Data indicated that both cells had a maximum proliferation when cultured in a medium containing 15% FBS at 100 cell/cm2. The isolated cells were readily differentiated into skeletal cells as were evident in specific staining as well as RT-PCR analysis. CONCLUSION: Supernatant of rat’s marrow primary culture contains mesenchymal stem cells (MSCs) with high growth rate.MR Baghaban Eslaminejad,H Nazarian,L TaghiyarBabol University of Medical Sciencesarticlemesenchymal stem cellsremoved mediumgrowth ratebonecartilage and adipose differentiationMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 10, Iss 2, Pp 12-22 (2008)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	mesenchymal stem cells removed medium growth rate bone cartilage and adipose differentiation Medicine R Medicine (General) R5-920
spellingShingle	mesenchymal stem cells removed medium growth rate bone cartilage and adipose differentiation Medicine R Medicine (General) R5-920 MR Baghaban Eslaminejad, H Nazarian, L Taghiyar MESENCHYMAL STEM CELLS WITH HIGH GROWTH RATE IN THE SUPERNATANT MEDIUM FROM RAT BONE MARROW PRIMARY CULTURE
description	BACKGROUND AND OBJECTIVE: In marrow primary culture for mesenchymal stem cell isolation, the cells suspended in supernatant are usually discarded at first medium exchange. The objective of this study was to culture and expand these cells, to examine their mesenchymal stem cell nature, to compare their growth rate with that of adherent cells of primary culture and eventually to optimize the culture conditions for the cells maximum proliferation.METHODS: In this experimental study, 10 Wistar strain rats were sacrificed, their tibial bone marrow were collected and plated in 75cm2 culture flasks. Four days after culture initiation, the supernatant medium was collected and its floating cells along with those primarily adherent cells were expanded by three successive subcultures, during which population doubling time (PDT) was calculated and statistically compared. Furthermore, culture condition including its fetal bovine serum (FBS) concentration as well as the cell density at culture initiation for maximum proliferation of the cells was optimized. The isolated cells were evaluated with respect to their potential to differentiate into bone, cartilage and adipose cells. FINDINGS: Based on our results, the population of the cells from removed medium was doubled in number each 14.9±2 hr, while this value was 19±2.1 hr for primarily adherent cell population (p<0.05). Data indicated that both cells had a maximum proliferation when cultured in a medium containing 15% FBS at 100 cell/cm2. The isolated cells were readily differentiated into skeletal cells as were evident in specific staining as well as RT-PCR analysis. CONCLUSION: Supernatant of rat’s marrow primary culture contains mesenchymal stem cells (MSCs) with high growth rate.
format	article
author	MR Baghaban Eslaminejad, H Nazarian, L Taghiyar
author_facet	MR Baghaban Eslaminejad, H Nazarian, L Taghiyar
author_sort	MR Baghaban Eslaminejad,
title	MESENCHYMAL STEM CELLS WITH HIGH GROWTH RATE IN THE SUPERNATANT MEDIUM FROM RAT BONE MARROW PRIMARY CULTURE
title_short	MESENCHYMAL STEM CELLS WITH HIGH GROWTH RATE IN THE SUPERNATANT MEDIUM FROM RAT BONE MARROW PRIMARY CULTURE
title_full	MESENCHYMAL STEM CELLS WITH HIGH GROWTH RATE IN THE SUPERNATANT MEDIUM FROM RAT BONE MARROW PRIMARY CULTURE
title_fullStr	MESENCHYMAL STEM CELLS WITH HIGH GROWTH RATE IN THE SUPERNATANT MEDIUM FROM RAT BONE MARROW PRIMARY CULTURE
title_full_unstemmed	MESENCHYMAL STEM CELLS WITH HIGH GROWTH RATE IN THE SUPERNATANT MEDIUM FROM RAT BONE MARROW PRIMARY CULTURE
title_sort	mesenchymal stem cells with high growth rate in the supernatant medium from rat bone marrow primary culture
publisher	Babol University of Medical Sciences
publishDate	2008
url	https://doaj.org/article/137ca52ad17e4d958c89f91400126b97
work_keys_str_mv	AT mrbaghabaneslaminejad mesenchymalstemcellswithhighgrowthrateinthesupernatantmediumfromratbonemarrowprimaryculture AT hnazarian mesenchymalstemcellswithhighgrowthrateinthesupernatantmediumfromratbonemarrowprimaryculture AT ltaghiyar mesenchymalstemcellswithhighgrowthrateinthesupernatantmediumfromratbonemarrowprimaryculture
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MESENCHYMAL STEM CELLS WITH HIGH GROWTH RATE IN THE SUPERNATANT MEDIUM FROM RAT BONE MARROW PRIMARY CULTURE

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