Assessment of the structural and functional characteristics of human mesenchymal stem cells associated with a prolonged exposure of morphine

Abstract The discovery of the expression of opioid receptors in the skin and their role in orchestrating the process of tissue repair gave rise to questions regarding the potential effects of clinical morphine treatment in wound healing. Although short term treatment was reported to improve tissue r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Francesco Carano, Gabriella Teti, Alessandra Ruggeri, Francesca Chiarini, Arianna Giorgetti, Maria C. Mazzotti, Paolo Fais, Mirella Falconi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/137e05914bf043ada2750a0bf2e6a58f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:137e05914bf043ada2750a0bf2e6a58f
record_format	dspace
spelling	oai:doaj.org-article:137e05914bf043ada2750a0bf2e6a58f2021-12-02T17:37:11ZAssessment of the structural and functional characteristics of human mesenchymal stem cells associated with a prolonged exposure of morphine10.1038/s41598-021-98682-62045-2322https://doaj.org/article/137e05914bf043ada2750a0bf2e6a58f2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98682-6https://doaj.org/toc/2045-2322Abstract The discovery of the expression of opioid receptors in the skin and their role in orchestrating the process of tissue repair gave rise to questions regarding the potential effects of clinical morphine treatment in wound healing. Although short term treatment was reported to improve tissue regeneration, in vivo chronic administration was associated to an impairment of the physiological healing process and systemic fibrosis. Human mesenchymal stem cells (hMSCs) play a fundamental role in tissue regeneration. In this regard, acute morphine exposition was recently reported to impact negatively on the functional characteristics of hMSCs, but little is currently known about its long-term effects. To determine how a prolonged treatment could impair their functional characteristics, we exposed hMSCs to increasing morphine concentrations respectively for nine and eighteen days, evaluating in particular the fibrogenic potential exerted by the long-term exposition. Our results showed a time dependent cell viability decline, and conditions compatible with a cellular senescent state. Ultrastructural and protein expression analysis were indicative of increased autophagy, suggesting a relation to a detoxification activity. In addition, the enhanced transcription observed for the genes involved in the synthesis and regulation of type I collagen suggested the possibility that a prolonged morphine treatment might exert its fibrotic potential risk, even involving the hMSCs.Francesco CaranoGabriella TetiAlessandra RuggeriFrancesca ChiariniArianna GiorgettiMaria C. MazzottiPaolo FaisMirella FalconiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francesco Carano Gabriella Teti Alessandra Ruggeri Francesca Chiarini Arianna Giorgetti Maria C. Mazzotti Paolo Fais Mirella Falconi Assessment of the structural and functional characteristics of human mesenchymal stem cells associated with a prolonged exposure of morphine
description	Abstract The discovery of the expression of opioid receptors in the skin and their role in orchestrating the process of tissue repair gave rise to questions regarding the potential effects of clinical morphine treatment in wound healing. Although short term treatment was reported to improve tissue regeneration, in vivo chronic administration was associated to an impairment of the physiological healing process and systemic fibrosis. Human mesenchymal stem cells (hMSCs) play a fundamental role in tissue regeneration. In this regard, acute morphine exposition was recently reported to impact negatively on the functional characteristics of hMSCs, but little is currently known about its long-term effects. To determine how a prolonged treatment could impair their functional characteristics, we exposed hMSCs to increasing morphine concentrations respectively for nine and eighteen days, evaluating in particular the fibrogenic potential exerted by the long-term exposition. Our results showed a time dependent cell viability decline, and conditions compatible with a cellular senescent state. Ultrastructural and protein expression analysis were indicative of increased autophagy, suggesting a relation to a detoxification activity. In addition, the enhanced transcription observed for the genes involved in the synthesis and regulation of type I collagen suggested the possibility that a prolonged morphine treatment might exert its fibrotic potential risk, even involving the hMSCs.
format	article
author	Francesco Carano Gabriella Teti Alessandra Ruggeri Francesca Chiarini Arianna Giorgetti Maria C. Mazzotti Paolo Fais Mirella Falconi
author_facet	Francesco Carano Gabriella Teti Alessandra Ruggeri Francesca Chiarini Arianna Giorgetti Maria C. Mazzotti Paolo Fais Mirella Falconi
author_sort	Francesco Carano
title	Assessment of the structural and functional characteristics of human mesenchymal stem cells associated with a prolonged exposure of morphine
title_short	Assessment of the structural and functional characteristics of human mesenchymal stem cells associated with a prolonged exposure of morphine
title_full	Assessment of the structural and functional characteristics of human mesenchymal stem cells associated with a prolonged exposure of morphine
title_fullStr	Assessment of the structural and functional characteristics of human mesenchymal stem cells associated with a prolonged exposure of morphine
title_full_unstemmed	Assessment of the structural and functional characteristics of human mesenchymal stem cells associated with a prolonged exposure of morphine
title_sort	assessment of the structural and functional characteristics of human mesenchymal stem cells associated with a prolonged exposure of morphine
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/137e05914bf043ada2750a0bf2e6a58f
work_keys_str_mv	AT francescocarano assessmentofthestructuralandfunctionalcharacteristicsofhumanmesenchymalstemcellsassociatedwithaprolongedexposureofmorphine AT gabriellateti assessmentofthestructuralandfunctionalcharacteristicsofhumanmesenchymalstemcellsassociatedwithaprolongedexposureofmorphine AT alessandraruggeri assessmentofthestructuralandfunctionalcharacteristicsofhumanmesenchymalstemcellsassociatedwithaprolongedexposureofmorphine AT francescachiarini assessmentofthestructuralandfunctionalcharacteristicsofhumanmesenchymalstemcellsassociatedwithaprolongedexposureofmorphine AT ariannagiorgetti assessmentofthestructuralandfunctionalcharacteristicsofhumanmesenchymalstemcellsassociatedwithaprolongedexposureofmorphine AT mariacmazzotti assessmentofthestructuralandfunctionalcharacteristicsofhumanmesenchymalstemcellsassociatedwithaprolongedexposureofmorphine AT paolofais assessmentofthestructuralandfunctionalcharacteristicsofhumanmesenchymalstemcellsassociatedwithaprolongedexposureofmorphine AT mirellafalconi assessmentofthestructuralandfunctionalcharacteristicsofhumanmesenchymalstemcellsassociatedwithaprolongedexposureofmorphine
_version_	1718379866728955904

Assessment of the structural and functional characteristics of human mesenchymal stem cells associated with a prolonged exposure of morphine

Ejemplares similares