Opportunities and challenges related to sperm cryopreservation in Atlantic salmon gene banks

Abstract Atlantic salmon are facing population declines and loss of productivity within populations due to anthropogenic impact factors and reduced survival at sea. Biobanking is an increasingly used tool to conserve the genetic integrity and diversity of populations threatened by extirpation. The a...

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Autores principales:	Kristin Bøe, Bjørn Bjøru, Marthe Tangvold Bårdsen, Anveig Nordtug Wist, Steffen Wolla, Arne Sivertsen
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Atlantic salmon effective population size fish conservation frozen gene banks live gene banks population restoration Ecology QH540-549.5 General. Including nature conservation, geographical distribution QH1-199.5
Acceso en línea:	https://doaj.org/article/13837b94a3ae4fcca22c2747776d54b0
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spelling	oai:doaj.org-article:13837b94a3ae4fcca22c2747776d54b02021-12-01T10:20:56ZOpportunities and challenges related to sperm cryopreservation in Atlantic salmon gene banks2578-485410.1111/csp2.552https://doaj.org/article/13837b94a3ae4fcca22c2747776d54b02021-12-01T00:00:00Zhttps://doi.org/10.1111/csp2.552https://doaj.org/toc/2578-4854Abstract Atlantic salmon are facing population declines and loss of productivity within populations due to anthropogenic impact factors and reduced survival at sea. Biobanking is an increasingly used tool to conserve the genetic integrity and diversity of populations threatened by extirpation. The aim of the current article is to discuss the opportunities and challenges that increased use of cryopreservation brings to biobanking activities, using the Norwegian Gene Bank (NGB) for Atlantic salmon as a model system. The NGB was established in 1985 and involves a traditional living gene bank, as well as “frozen gene bank” where paternal germplasm is stored as cryopreserved sperm. Cryopreservation is a method where cells or tissues are frozen in liquid nitrogen to temperatures where all biological processes are paused, thus allowing the cells to remain viable after later warming/thawing to temperatures above 0°C. Cryopreservation is therefore used in long‐term preservation of genetic diversity and characteristics of wild populations. Until recently, implementation of large scale use of cryopreserved sperm in the live gene bank has been limited by a lack of protocols/capacity to preserve larger portions of sperms. More recent developments in cryopreservation methodologies, now enables preservation of samples sufficient for mass fertilization. Mass fertilization by cryopreserved sperm opens new opportunities to gene bank operations, including increased capacity to restore lost populations, mitigation of genetic changes in broodstock fish, as well as increased capacity at live gene bank facilities through the replacement of older males with frozen sperm. Knowledge demands regarding potential genetic damage to cryopreserved milt and potential epigenetic effects caused by the cryopreservation procedure should, however, be addressed.Kristin BøeBjørn BjøruMarthe Tangvold BårdsenAnveig Nordtug WistSteffen WollaArne SivertsenWileyarticleAtlantic salmoneffective population sizefish conservationfrozen gene bankslive gene bankspopulation restorationEcologyQH540-549.5General. Including nature conservation, geographical distributionQH1-199.5ENConservation Science and Practice, Vol 3, Iss 12, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Atlantic salmon effective population size fish conservation frozen gene banks live gene banks population restoration Ecology QH540-549.5 General. Including nature conservation, geographical distribution QH1-199.5
spellingShingle	Atlantic salmon effective population size fish conservation frozen gene banks live gene banks population restoration Ecology QH540-549.5 General. Including nature conservation, geographical distribution QH1-199.5 Kristin Bøe Bjørn Bjøru Marthe Tangvold Bårdsen Anveig Nordtug Wist Steffen Wolla Arne Sivertsen Opportunities and challenges related to sperm cryopreservation in Atlantic salmon gene banks
description	Abstract Atlantic salmon are facing population declines and loss of productivity within populations due to anthropogenic impact factors and reduced survival at sea. Biobanking is an increasingly used tool to conserve the genetic integrity and diversity of populations threatened by extirpation. The aim of the current article is to discuss the opportunities and challenges that increased use of cryopreservation brings to biobanking activities, using the Norwegian Gene Bank (NGB) for Atlantic salmon as a model system. The NGB was established in 1985 and involves a traditional living gene bank, as well as “frozen gene bank” where paternal germplasm is stored as cryopreserved sperm. Cryopreservation is a method where cells or tissues are frozen in liquid nitrogen to temperatures where all biological processes are paused, thus allowing the cells to remain viable after later warming/thawing to temperatures above 0°C. Cryopreservation is therefore used in long‐term preservation of genetic diversity and characteristics of wild populations. Until recently, implementation of large scale use of cryopreserved sperm in the live gene bank has been limited by a lack of protocols/capacity to preserve larger portions of sperms. More recent developments in cryopreservation methodologies, now enables preservation of samples sufficient for mass fertilization. Mass fertilization by cryopreserved sperm opens new opportunities to gene bank operations, including increased capacity to restore lost populations, mitigation of genetic changes in broodstock fish, as well as increased capacity at live gene bank facilities through the replacement of older males with frozen sperm. Knowledge demands regarding potential genetic damage to cryopreserved milt and potential epigenetic effects caused by the cryopreservation procedure should, however, be addressed.
format	article
author	Kristin Bøe Bjørn Bjøru Marthe Tangvold Bårdsen Anveig Nordtug Wist Steffen Wolla Arne Sivertsen
author_facet	Kristin Bøe Bjørn Bjøru Marthe Tangvold Bårdsen Anveig Nordtug Wist Steffen Wolla Arne Sivertsen
author_sort	Kristin Bøe
title	Opportunities and challenges related to sperm cryopreservation in Atlantic salmon gene banks
title_short	Opportunities and challenges related to sperm cryopreservation in Atlantic salmon gene banks
title_full	Opportunities and challenges related to sperm cryopreservation in Atlantic salmon gene banks
title_fullStr	Opportunities and challenges related to sperm cryopreservation in Atlantic salmon gene banks
title_full_unstemmed	Opportunities and challenges related to sperm cryopreservation in Atlantic salmon gene banks
title_sort	opportunities and challenges related to sperm cryopreservation in atlantic salmon gene banks
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/13837b94a3ae4fcca22c2747776d54b0
work_keys_str_mv	AT kristinbøe opportunitiesandchallengesrelatedtospermcryopreservationinatlanticsalmongenebanks AT bjørnbjøru opportunitiesandchallengesrelatedtospermcryopreservationinatlanticsalmongenebanks AT marthetangvoldbardsen opportunitiesandchallengesrelatedtospermcryopreservationinatlanticsalmongenebanks AT anveignordtugwist opportunitiesandchallengesrelatedtospermcryopreservationinatlanticsalmongenebanks AT steffenwolla opportunitiesandchallengesrelatedtospermcryopreservationinatlanticsalmongenebanks AT arnesivertsen opportunitiesandchallengesrelatedtospermcryopreservationinatlanticsalmongenebanks
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Opportunities and challenges related to sperm cryopreservation in Atlantic salmon gene banks

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