Identification of enriched hyperthermophilic microbial communities from a deep-sea hydrothermal vent chimney under electrolithoautotrophic culture conditions
Abstract Deep-sea hydrothermal vents are extreme and complex ecosystems based on a trophic chain. We are still unsure of the identities of the first colonizers of these environments and their metabolism, but they are thought to be (hyper)thermophilic autotrophs. Here we investigate whether the elect...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/13847b8a085149da80bb10aea8f90e98 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:13847b8a085149da80bb10aea8f90e98 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:13847b8a085149da80bb10aea8f90e982021-12-02T16:26:22ZIdentification of enriched hyperthermophilic microbial communities from a deep-sea hydrothermal vent chimney under electrolithoautotrophic culture conditions10.1038/s41598-021-94135-22045-2322https://doaj.org/article/13847b8a085149da80bb10aea8f90e982021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94135-2https://doaj.org/toc/2045-2322Abstract Deep-sea hydrothermal vents are extreme and complex ecosystems based on a trophic chain. We are still unsure of the identities of the first colonizers of these environments and their metabolism, but they are thought to be (hyper)thermophilic autotrophs. Here we investigate whether the electric potential observed across hydrothermal chimneys could serve as an energy source for these first colonizers. Experiments were performed in a two-chamber microbial electrochemical system inoculated with deep-sea hydrothermal chimney samples, with a cathode as sole electron donor, CO2 as sole carbon source, and nitrate, sulfate, or oxygen as electron acceptors. After a few days of culturing, all three experiments showed growth of electrotrophic biofilms consuming the electrons (directly or indirectly) and producing organic compounds including acetate, glycerol, and pyruvate. Within the biofilms, the only known autotroph species retrieved were members of Archaeoglobales. Various heterotrophic phyla also grew through trophic interactions, with Thermococcales growing in all three experiments as well as other bacterial groups specific to each electron acceptor. This electrotrophic metabolism as energy source driving initial microbial colonization of conductive hydrothermal chimneys is discussed.Guillaume PillotOulfat Amin AliSylvain DavidsonLaetitia ShintuAnne GodfroyYannick Combet-BlancPatricia BoninPierre-Pol LiebgottNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Guillaume Pillot Oulfat Amin Ali Sylvain Davidson Laetitia Shintu Anne Godfroy Yannick Combet-Blanc Patricia Bonin Pierre-Pol Liebgott Identification of enriched hyperthermophilic microbial communities from a deep-sea hydrothermal vent chimney under electrolithoautotrophic culture conditions |
| description |
Abstract Deep-sea hydrothermal vents are extreme and complex ecosystems based on a trophic chain. We are still unsure of the identities of the first colonizers of these environments and their metabolism, but they are thought to be (hyper)thermophilic autotrophs. Here we investigate whether the electric potential observed across hydrothermal chimneys could serve as an energy source for these first colonizers. Experiments were performed in a two-chamber microbial electrochemical system inoculated with deep-sea hydrothermal chimney samples, with a cathode as sole electron donor, CO2 as sole carbon source, and nitrate, sulfate, or oxygen as electron acceptors. After a few days of culturing, all three experiments showed growth of electrotrophic biofilms consuming the electrons (directly or indirectly) and producing organic compounds including acetate, glycerol, and pyruvate. Within the biofilms, the only known autotroph species retrieved were members of Archaeoglobales. Various heterotrophic phyla also grew through trophic interactions, with Thermococcales growing in all three experiments as well as other bacterial groups specific to each electron acceptor. This electrotrophic metabolism as energy source driving initial microbial colonization of conductive hydrothermal chimneys is discussed. |
| format |
article |
| author |
Guillaume Pillot Oulfat Amin Ali Sylvain Davidson Laetitia Shintu Anne Godfroy Yannick Combet-Blanc Patricia Bonin Pierre-Pol Liebgott |
| author_facet |
Guillaume Pillot Oulfat Amin Ali Sylvain Davidson Laetitia Shintu Anne Godfroy Yannick Combet-Blanc Patricia Bonin Pierre-Pol Liebgott |
| author_sort |
Guillaume Pillot |
| title |
Identification of enriched hyperthermophilic microbial communities from a deep-sea hydrothermal vent chimney under electrolithoautotrophic culture conditions |
| title_short |
Identification of enriched hyperthermophilic microbial communities from a deep-sea hydrothermal vent chimney under electrolithoautotrophic culture conditions |
| title_full |
Identification of enriched hyperthermophilic microbial communities from a deep-sea hydrothermal vent chimney under electrolithoautotrophic culture conditions |
| title_fullStr |
Identification of enriched hyperthermophilic microbial communities from a deep-sea hydrothermal vent chimney under electrolithoautotrophic culture conditions |
| title_full_unstemmed |
Identification of enriched hyperthermophilic microbial communities from a deep-sea hydrothermal vent chimney under electrolithoautotrophic culture conditions |
| title_sort |
identification of enriched hyperthermophilic microbial communities from a deep-sea hydrothermal vent chimney under electrolithoautotrophic culture conditions |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/13847b8a085149da80bb10aea8f90e98 |
| work_keys_str_mv |
AT guillaumepillot identificationofenrichedhyperthermophilicmicrobialcommunitiesfromadeepseahydrothermalventchimneyunderelectrolithoautotrophiccultureconditions AT oulfataminali identificationofenrichedhyperthermophilicmicrobialcommunitiesfromadeepseahydrothermalventchimneyunderelectrolithoautotrophiccultureconditions AT sylvaindavidson identificationofenrichedhyperthermophilicmicrobialcommunitiesfromadeepseahydrothermalventchimneyunderelectrolithoautotrophiccultureconditions AT laetitiashintu identificationofenrichedhyperthermophilicmicrobialcommunitiesfromadeepseahydrothermalventchimneyunderelectrolithoautotrophiccultureconditions AT annegodfroy identificationofenrichedhyperthermophilicmicrobialcommunitiesfromadeepseahydrothermalventchimneyunderelectrolithoautotrophiccultureconditions AT yannickcombetblanc identificationofenrichedhyperthermophilicmicrobialcommunitiesfromadeepseahydrothermalventchimneyunderelectrolithoautotrophiccultureconditions AT patriciabonin identificationofenrichedhyperthermophilicmicrobialcommunitiesfromadeepseahydrothermalventchimneyunderelectrolithoautotrophiccultureconditions AT pierrepolliebgott identificationofenrichedhyperthermophilicmicrobialcommunitiesfromadeepseahydrothermalventchimneyunderelectrolithoautotrophiccultureconditions |
| _version_ |
1718384036505714688 |