Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)

Previous attempts to combine expansion microscopy (ExM) and single molecule localisation microscopy (SMLM) have proved challenging. Here the authors show that post-labelling Ex-SMLM improves labelling efficiency, reduces linkage error, and preserves ultrastructural details.

Guardado en:

Detalles Bibliográficos
Autores principales:	Fabian U. Zwettler, Sebastian Reinhard, Davide Gambarotto, Toby D. M. Bell, Virginie Hamel, Paul Guichard, Markus Sauer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/138518bbdfb845248b9023cf52af2865
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:138518bbdfb845248b9023cf52af2865
record_format	dspace
spelling	oai:doaj.org-article:138518bbdfb845248b9023cf52af28652021-12-02T15:23:19ZMolecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)10.1038/s41467-020-17086-82041-1723https://doaj.org/article/138518bbdfb845248b9023cf52af28652020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17086-8https://doaj.org/toc/2041-1723Previous attempts to combine expansion microscopy (ExM) and single molecule localisation microscopy (SMLM) have proved challenging. Here the authors show that post-labelling Ex-SMLM improves labelling efficiency, reduces linkage error, and preserves ultrastructural details.Fabian U. ZwettlerSebastian ReinhardDavide GambarottoToby D. M. BellVirginie HamelPaul GuichardMarkus SauerNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Fabian U. Zwettler Sebastian Reinhard Davide Gambarotto Toby D. M. Bell Virginie Hamel Paul Guichard Markus Sauer Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)
description	Previous attempts to combine expansion microscopy (ExM) and single molecule localisation microscopy (SMLM) have proved challenging. Here the authors show that post-labelling Ex-SMLM improves labelling efficiency, reduces linkage error, and preserves ultrastructural details.
format	article
author	Fabian U. Zwettler Sebastian Reinhard Davide Gambarotto Toby D. M. Bell Virginie Hamel Paul Guichard Markus Sauer
author_facet	Fabian U. Zwettler Sebastian Reinhard Davide Gambarotto Toby D. M. Bell Virginie Hamel Paul Guichard Markus Sauer
author_sort	Fabian U. Zwettler
title	Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)
title_short	Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)
title_full	Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)
title_fullStr	Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)
title_full_unstemmed	Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)
title_sort	molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (ex-smlm)
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/138518bbdfb845248b9023cf52af2865
work_keys_str_mv	AT fabianuzwettler molecularresolutionimagingbypostlabelingexpansionsinglemoleculelocalizationmicroscopyexsmlm AT sebastianreinhard molecularresolutionimagingbypostlabelingexpansionsinglemoleculelocalizationmicroscopyexsmlm AT davidegambarotto molecularresolutionimagingbypostlabelingexpansionsinglemoleculelocalizationmicroscopyexsmlm AT tobydmbell molecularresolutionimagingbypostlabelingexpansionsinglemoleculelocalizationmicroscopyexsmlm AT virginiehamel molecularresolutionimagingbypostlabelingexpansionsinglemoleculelocalizationmicroscopyexsmlm AT paulguichard molecularresolutionimagingbypostlabelingexpansionsinglemoleculelocalizationmicroscopyexsmlm AT markussauer molecularresolutionimagingbypostlabelingexpansionsinglemoleculelocalizationmicroscopyexsmlm
_version_	1718387247756083200

Molecular resolution imaging by post-labeling expansion single-molecule localization microscopy (Ex-SMLM)

Ejemplares similares