Dynamic patterns of YAP1 expression and cellular localization in the developing and injured utricle

Abstract The Hippo signaling pathway is a key regulator of tissue development and regeneration. Activation of the Hippo pathway leads to nuclear translocation of the YAP1 transcriptional coactivator, resulting in changes in gene expression and cell cycle entry. Recent studies have demonstrated the n...

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Autores principales:	Vikrant Borse, Matthew Barton, Harry Arndt, Tejbeer Kaur, Mark E. Warchol
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1389d643523a4e9d91015cc40f9d3605
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spelling	oai:doaj.org-article:1389d643523a4e9d91015cc40f9d36052021-12-02T13:24:26ZDynamic patterns of YAP1 expression and cellular localization in the developing and injured utricle10.1038/s41598-020-77775-82045-2322https://doaj.org/article/1389d643523a4e9d91015cc40f9d36052021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77775-8https://doaj.org/toc/2045-2322Abstract The Hippo signaling pathway is a key regulator of tissue development and regeneration. Activation of the Hippo pathway leads to nuclear translocation of the YAP1 transcriptional coactivator, resulting in changes in gene expression and cell cycle entry. Recent studies have demonstrated the nuclear translocation of YAP1 during the development of the sensory organs of the inner ear, but the possible role of YAP1 in sensory regeneration of the inner ear is unclear. The present study characterized the cellular localization of YAP1 in the utricles of mice and chicks, both under normal conditions and after HC injury. During neonatal development, YAP1 expression was observed in the cytoplasm of supporting cells, and was transiently expressed in the cytoplasm of some differentiating hair cells. We also observed temporary nuclear translocation of YAP1 in supporting cells of the mouse utricle after short periods in organotypic culture. However, little or no nuclear translocation of YAP1 was observed in the utricles of neonatal or mature mice after ototoxic injury. In contrast, substantial YAP1 nuclear translocation was observed in the chicken utricle after streptomycin treatment in vitro and in vivo. Together, these data suggest that differences in YAP1 signaling may partially account for the differing regenerative abilities of the avian vs. mammalian inner ear.Vikrant BorseMatthew BartonHarry ArndtTejbeer KaurMark E. WarcholNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vikrant Borse Matthew Barton Harry Arndt Tejbeer Kaur Mark E. Warchol Dynamic patterns of YAP1 expression and cellular localization in the developing and injured utricle
description	Abstract The Hippo signaling pathway is a key regulator of tissue development and regeneration. Activation of the Hippo pathway leads to nuclear translocation of the YAP1 transcriptional coactivator, resulting in changes in gene expression and cell cycle entry. Recent studies have demonstrated the nuclear translocation of YAP1 during the development of the sensory organs of the inner ear, but the possible role of YAP1 in sensory regeneration of the inner ear is unclear. The present study characterized the cellular localization of YAP1 in the utricles of mice and chicks, both under normal conditions and after HC injury. During neonatal development, YAP1 expression was observed in the cytoplasm of supporting cells, and was transiently expressed in the cytoplasm of some differentiating hair cells. We also observed temporary nuclear translocation of YAP1 in supporting cells of the mouse utricle after short periods in organotypic culture. However, little or no nuclear translocation of YAP1 was observed in the utricles of neonatal or mature mice after ototoxic injury. In contrast, substantial YAP1 nuclear translocation was observed in the chicken utricle after streptomycin treatment in vitro and in vivo. Together, these data suggest that differences in YAP1 signaling may partially account for the differing regenerative abilities of the avian vs. mammalian inner ear.
format	article
author	Vikrant Borse Matthew Barton Harry Arndt Tejbeer Kaur Mark E. Warchol
author_facet	Vikrant Borse Matthew Barton Harry Arndt Tejbeer Kaur Mark E. Warchol
author_sort	Vikrant Borse
title	Dynamic patterns of YAP1 expression and cellular localization in the developing and injured utricle
title_short	Dynamic patterns of YAP1 expression and cellular localization in the developing and injured utricle
title_full	Dynamic patterns of YAP1 expression and cellular localization in the developing and injured utricle
title_fullStr	Dynamic patterns of YAP1 expression and cellular localization in the developing and injured utricle
title_full_unstemmed	Dynamic patterns of YAP1 expression and cellular localization in the developing and injured utricle
title_sort	dynamic patterns of yap1 expression and cellular localization in the developing and injured utricle
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/1389d643523a4e9d91015cc40f9d3605
work_keys_str_mv	AT vikrantborse dynamicpatternsofyap1expressionandcellularlocalizationinthedevelopingandinjuredutricle AT matthewbarton dynamicpatternsofyap1expressionandcellularlocalizationinthedevelopingandinjuredutricle AT harryarndt dynamicpatternsofyap1expressionandcellularlocalizationinthedevelopingandinjuredutricle AT tejbeerkaur dynamicpatternsofyap1expressionandcellularlocalizationinthedevelopingandinjuredutricle AT markewarchol dynamicpatternsofyap1expressionandcellularlocalizationinthedevelopingandinjuredutricle
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Dynamic patterns of YAP1 expression and cellular localization in the developing and injured utricle

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