Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography

To study Mycobacterium tuberculosis (MTB) genotypes circulating in the Sakha Republic (Yakutia), molecular genetic analysis of multidrug-resistant MTB was performed using real-time PCR-test. The study showed predominance of Beijing genotype with resistance to isoniazid caused by mutations in kat G g...

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Autores principales:	M. K. Vinokurova, O. E. Dogorova, I. S. Malogulova, E. S. Pavlova
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2018
Materias:	mycobacterium tuberculosis genotype isoniazid drug administration routes high pressure liquid chromatography Science Q
Acceso en línea:	https://doaj.org/article/13919349b6e44caebcb4d12810ee8825
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spelling	oai:doaj.org-article:13919349b6e44caebcb4d12810ee88252021-11-23T06:14:40ZDetection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography2541-94202587-959610.29413/ABS.2018-3.2.13https://doaj.org/article/13919349b6e44caebcb4d12810ee88252018-04-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/563https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596To study Mycobacterium tuberculosis (MTB) genotypes circulating in the Sakha Republic (Yakutia), molecular genetic analysis of multidrug-resistant MTB was performed using real-time PCR-test. The study showed predominance of Beijing genotype with resistance to isoniazid caused by mutations in kat G gene alone (86.2 %), in both kat G and inh A genes (10.3 %), or in inh A alone (3,5 %). In this work, we studied variations in serum isoniazid concentration in patients with multidrug-resistant tuberculosis, using different routes of drug administration: regional lymphotropic, intravenous infusion (IV), intramuscular injection (IM), and oral. Blood samples were obtained 1.5, 6, and 9 hours after administration of the drug. Levels and variations of isoniazid serum concentrations were assessed at intervals, using high-performance liquid chromatography (HPLC). For assessing the efficiency of treatment, patients were divided to four groups according to isoniazid administration routes. The study established that serum isoniazid concentrations observed with regional lymphotropic administration, were initially the lowest (4.2 mg/L), compared to IV infusion, IM, and oral administration routes (8,12.5, and 17.1 mg/L, respectively), but showed slower reduction of concentration. It was also noticed, that after 9 hours, the serum concentration of isoniazid was reliably higher in regional lymphotropicgroup, than in the rest of study groups (2.2 vs. 0.8 mg/L). Higher therapeutic effect of intralymphatic drug administration could be explained by the formation within the regional lymphatic network of a localized area around the site of injection containing enhanced concentration of isoniazid and allowing extended drug release into bloodstream, resulting further in prolonged drug biotransformation.M. K. VinokurovaO. E. DogorovaI. S. MalogulovaE. S. PavlovaScientific Сentre for Family Health and Human Reproduction Problemsarticlemycobacterium tuberculosisgenotypeisoniaziddrug administration routeshigh pressure liquid chromatographyScienceQRUActa Biomedica Scientifica, Vol 3, Iss 2, Pp 75-79 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	mycobacterium tuberculosis genotype isoniazid drug administration routes high pressure liquid chromatography Science Q
spellingShingle	mycobacterium tuberculosis genotype isoniazid drug administration routes high pressure liquid chromatography Science Q M. K. Vinokurova O. E. Dogorova I. S. Malogulova E. S. Pavlova Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography
description	To study Mycobacterium tuberculosis (MTB) genotypes circulating in the Sakha Republic (Yakutia), molecular genetic analysis of multidrug-resistant MTB was performed using real-time PCR-test. The study showed predominance of Beijing genotype with resistance to isoniazid caused by mutations in kat G gene alone (86.2 %), in both kat G and inh A genes (10.3 %), or in inh A alone (3,5 %). In this work, we studied variations in serum isoniazid concentration in patients with multidrug-resistant tuberculosis, using different routes of drug administration: regional lymphotropic, intravenous infusion (IV), intramuscular injection (IM), and oral. Blood samples were obtained 1.5, 6, and 9 hours after administration of the drug. Levels and variations of isoniazid serum concentrations were assessed at intervals, using high-performance liquid chromatography (HPLC). For assessing the efficiency of treatment, patients were divided to four groups according to isoniazid administration routes. The study established that serum isoniazid concentrations observed with regional lymphotropic administration, were initially the lowest (4.2 mg/L), compared to IV infusion, IM, and oral administration routes (8,12.5, and 17.1 mg/L, respectively), but showed slower reduction of concentration. It was also noticed, that after 9 hours, the serum concentration of isoniazid was reliably higher in regional lymphotropicgroup, than in the rest of study groups (2.2 vs. 0.8 mg/L). Higher therapeutic effect of intralymphatic drug administration could be explained by the formation within the regional lymphatic network of a localized area around the site of injection containing enhanced concentration of isoniazid and allowing extended drug release into bloodstream, resulting further in prolonged drug biotransformation.
format	article
author	M. K. Vinokurova O. E. Dogorova I. S. Malogulova E. S. Pavlova
author_facet	M. K. Vinokurova O. E. Dogorova I. S. Malogulova E. S. Pavlova
author_sort	M. K. Vinokurova
title	Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography
title_short	Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography
title_full	Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography
title_fullStr	Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography
title_full_unstemmed	Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography
title_sort	detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2018
url	https://doaj.org/article/13919349b6e44caebcb4d12810ee8825
work_keys_str_mv	AT mkvinokurova detectionofserumisoniazidconcentrationinpatientswithmultidrugresistanttuberculosisusinghighperformanceliquidchromatography AT oedogorova detectionofserumisoniazidconcentrationinpatientswithmultidrugresistanttuberculosisusinghighperformanceliquidchromatography AT ismalogulova detectionofserumisoniazidconcentrationinpatientswithmultidrugresistanttuberculosisusinghighperformanceliquidchromatography AT espavlova detectionofserumisoniazidconcentrationinpatientswithmultidrugresistanttuberculosisusinghighperformanceliquidchromatography
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Detection of serum isoniazid concentration in patients with multidrug-resistant tuberculosis using high-performance liquid chromatography

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