Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice

Background: Several caloric restriction studies revealed good for diabetes prevention. However, prevalence of it seems rising yearly. It needs alternative technique thus people can choose suitable way for them. Aim: To determine the effect of glucose diet intermittently on pancreatic duodenal homeo...

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Autores principales:	Lilik Herawati, Kristanti Wanito Wigati, Purwo Sri Rejeki, Widjiati Widjiati, Roedi Irawan
Formato:	article
Lenguaje:	EN RU
Publicado:	Endocrinology Research Centre 2018
Materias:	pdx-1 apoptosis pancreatic area glucose, insulin Nutritional diseases. Deficiency diseases RC620-627
Acceso en línea:	https://doaj.org/article/1393bd0d66de49aebd4325ec16de3a22
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spelling	oai:doaj.org-article:1393bd0d66de49aebd4325ec16de3a222021-11-14T09:00:22ZIncreased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice2072-03512072-037810.14341/DM9437https://doaj.org/article/1393bd0d66de49aebd4325ec16de3a222018-12-01T00:00:00Zhttps://www.dia-endojournals.ru/jour/article/view/9437https://doaj.org/toc/2072-0351https://doaj.org/toc/2072-0378Background: Several caloric restriction studies revealed good for diabetes prevention. However, prevalence of it seems rising yearly. It needs alternative technique thus people can choose suitable way for them. Aim: To determine the effect of glucose diet intermittently on pancreatic duodenal homeobox-1 (PDX-1), apoptosis in pancreatic islets, and pancreatic islets area. Materials and methods: Balb/c mice were divided into five groups. Control group was given standard diet. The Continuous group was given standard diet and added with 7.4% calories continuously. The 1x, 2x, and 3x intermittent groups were given standard diet and added 7.4% calories for 1x, 2x, and 3x/week respectively. The 7.4% calorie addition was a glucose solution by oral galvage and ad libitum for 8 weeks. Results: There was a significantly difference on apoptosis density (p=0.043), but not in PDX-1. The islets Int2x and Int3x groups showed a significant decrease than control group (p=0.048). Insulin serum levels increased significantly in Continuous group compared to control group (p=0.04). In addition, the insulin serum level of 1x and 3x intermittent groups were significantly lower than Continuous group (p<0.05). Pre-post blood glucose levels on treatment groups decreased significantly compared to control group (p=0.012). Conclusions: Continuous and 1-3x/week intermittent addition of 7.4% calories of glucose for 8 weeks indicate a compensation mechanism for maintaining homeostasis, such as increase insulin serum level and seem to initiate the changes of morphologic-biomolecular (mainly apoptosis density in islets). The better mode is 1x/week of additional calories. However it needs further exploration to find out other influenced factors for these mechanism discovery.Lilik HerawatiKristanti Wanito WigatiPurwo Sri RejekiWidjiati WidjiatiRoedi IrawanEndocrinology Research Centrearticlepdx-1apoptosispancreatic areaglucose, insulinNutritional diseases. Deficiency diseasesRC620-627ENRUСахарный диабет, Vol 21, Iss 6, Pp 497-505 (2018)
institution	DOAJ
collection	DOAJ
language	EN RU
topic	pdx-1 apoptosis pancreatic area glucose, insulin Nutritional diseases. Deficiency diseases RC620-627
spellingShingle	pdx-1 apoptosis pancreatic area glucose, insulin Nutritional diseases. Deficiency diseases RC620-627 Lilik Herawati Kristanti Wanito Wigati Purwo Sri Rejeki Widjiati Widjiati Roedi Irawan Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice
description	Background: Several caloric restriction studies revealed good for diabetes prevention. However, prevalence of it seems rising yearly. It needs alternative technique thus people can choose suitable way for them. Aim: To determine the effect of glucose diet intermittently on pancreatic duodenal homeobox-1 (PDX-1), apoptosis in pancreatic islets, and pancreatic islets area. Materials and methods: Balb/c mice were divided into five groups. Control group was given standard diet. The Continuous group was given standard diet and added with 7.4% calories continuously. The 1x, 2x, and 3x intermittent groups were given standard diet and added 7.4% calories for 1x, 2x, and 3x/week respectively. The 7.4% calorie addition was a glucose solution by oral galvage and ad libitum for 8 weeks. Results: There was a significantly difference on apoptosis density (p=0.043), but not in PDX-1. The islets Int2x and Int3x groups showed a significant decrease than control group (p=0.048). Insulin serum levels increased significantly in Continuous group compared to control group (p=0.04). In addition, the insulin serum level of 1x and 3x intermittent groups were significantly lower than Continuous group (p<0.05). Pre-post blood glucose levels on treatment groups decreased significantly compared to control group (p=0.012). Conclusions: Continuous and 1-3x/week intermittent addition of 7.4% calories of glucose for 8 weeks indicate a compensation mechanism for maintaining homeostasis, such as increase insulin serum level and seem to initiate the changes of morphologic-biomolecular (mainly apoptosis density in islets). The better mode is 1x/week of additional calories. However it needs further exploration to find out other influenced factors for these mechanism discovery.
format	article
author	Lilik Herawati Kristanti Wanito Wigati Purwo Sri Rejeki Widjiati Widjiati Roedi Irawan
author_facet	Lilik Herawati Kristanti Wanito Wigati Purwo Sri Rejeki Widjiati Widjiati Roedi Irawan
author_sort	Lilik Herawati
title	Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice
title_short	Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice
title_full	Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice
title_fullStr	Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice
title_full_unstemmed	Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice
title_sort	increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice
publisher	Endocrinology Research Centre
publishDate	2018
url	https://doaj.org/article/1393bd0d66de49aebd4325ec16de3a22
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Increased apoptosis, but not pancreatic duodenal homeobox-1 expression in pancreatic islets is associated with intermittent glucose loads in mice

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