Body temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry

Abstract Body temperature is a valuable parameter in determining the wellbeing of laboratory animals. However, using body temperature to refine humane endpoints during acute illness generally lacks comprehensiveness and exposes to inter-observer bias. Here we compared two methods to assess body temp...

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Autores principales:	Jie Mei, Nico Riedel, Ulrike Grittner, Matthias Endres, Stefanie Banneke, Julius Valentin Emmrich
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/1394e3f9026845f29dbcbab6a4cadfac
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spelling	oai:doaj.org-article:1394e3f9026845f29dbcbab6a4cadfac2021-12-02T11:40:47ZBody temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry10.1038/s41598-018-22020-62045-2322https://doaj.org/article/1394e3f9026845f29dbcbab6a4cadfac2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-22020-6https://doaj.org/toc/2045-2322Abstract Body temperature is a valuable parameter in determining the wellbeing of laboratory animals. However, using body temperature to refine humane endpoints during acute illness generally lacks comprehensiveness and exposes to inter-observer bias. Here we compared two methods to assess body temperature in mice, namely implanted radio frequency identification (RFID) temperature transponders (method 1) to non-contact infrared thermometry (method 2) in 435 mice for up to 7 days during normothermia and lipopolysaccharide (LPS) endotoxin-induced hypothermia. There was excellent agreement between core and surface temperature as determined by method 1 and 2, respectively, whereas the intra- and inter-subject variation was higher for method 2. Nevertheless, using machine learning algorithms to determine temperature-based endpoints both methods had excellent accuracy in predicting death as an outcome event. Therefore, less expensive and cumbersome non-contact infrared thermometry can serve as a reliable alternative for implantable transponder-based systems for hypothermic responses, although requiring standardization between experimenters.Jie MeiNico RiedelUlrike GrittnerMatthias EndresStefanie BannekeJulius Valentin EmmrichNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jie Mei Nico Riedel Ulrike Grittner Matthias Endres Stefanie Banneke Julius Valentin Emmrich Body temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry
description	Abstract Body temperature is a valuable parameter in determining the wellbeing of laboratory animals. However, using body temperature to refine humane endpoints during acute illness generally lacks comprehensiveness and exposes to inter-observer bias. Here we compared two methods to assess body temperature in mice, namely implanted radio frequency identification (RFID) temperature transponders (method 1) to non-contact infrared thermometry (method 2) in 435 mice for up to 7 days during normothermia and lipopolysaccharide (LPS) endotoxin-induced hypothermia. There was excellent agreement between core and surface temperature as determined by method 1 and 2, respectively, whereas the intra- and inter-subject variation was higher for method 2. Nevertheless, using machine learning algorithms to determine temperature-based endpoints both methods had excellent accuracy in predicting death as an outcome event. Therefore, less expensive and cumbersome non-contact infrared thermometry can serve as a reliable alternative for implantable transponder-based systems for hypothermic responses, although requiring standardization between experimenters.
format	article
author	Jie Mei Nico Riedel Ulrike Grittner Matthias Endres Stefanie Banneke Julius Valentin Emmrich
author_facet	Jie Mei Nico Riedel Ulrike Grittner Matthias Endres Stefanie Banneke Julius Valentin Emmrich
author_sort	Jie Mei
title	Body temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry
title_short	Body temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry
title_full	Body temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry
title_fullStr	Body temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry
title_full_unstemmed	Body temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry
title_sort	body temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/1394e3f9026845f29dbcbab6a4cadfac
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_version_	1718395504542351360

Body temperature measurement in mice during acute illness: implantable temperature transponder versus surface infrared thermometry

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