3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model

Abstract Bone regeneration is a complex process and the clinical translation of tissue engineered constructs (TECs) remains a challenge. The combination of biomaterials and mesenchymal stem cells (MSCs) may enhance the healing process through paracrine effects. Here, we investigated the influence of...

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Autores principales:	Lukas Otto, Petra Wolint, Annina Bopp, Anna Woloszyk, Anton S. Becker, Andreas Boss, Roland Böni, Maurizio Calcagni, Pietro Giovanoli, Simon P. Hoerstrup, Maximilian Y. Emmert, Johanna Buschmann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/13a7a4e40eaf4c73bc17ec0a47a77cc5
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spelling	oai:doaj.org-article:13a7a4e40eaf4c73bc17ec0a47a77cc52021-12-02T11:37:18Z3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model10.1038/s41598-021-84123-x2045-2322https://doaj.org/article/13a7a4e40eaf4c73bc17ec0a47a77cc52021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84123-xhttps://doaj.org/toc/2045-2322Abstract Bone regeneration is a complex process and the clinical translation of tissue engineered constructs (TECs) remains a challenge. The combination of biomaterials and mesenchymal stem cells (MSCs) may enhance the healing process through paracrine effects. Here, we investigated the influence of cell format in combination with a collagen scaffold on key factors in bone healing process, such as mineralization, cell infiltration, vascularization, and ECM production. MSCs as single cells (2D-SCs), assembled into microtissues (3D-MTs) or their corresponding secretomes were combined with a collagen scaffold and incubated on the chicken embryo chorioallantoic membrane (CAM) for 7 days. A comprehensive quantitative analysis was performed on a cellular level by histology and by microcomputed tomography (microCT). In all experimental groups, accumulation of collagen and glycosaminoglycan within the scaffold was observed over time. A pronounced cell infiltration and vascularization from the interface to the surface region of the CAM was detected. The 3D-MT secretome showed a significant mineralization of the biomaterial using microCT compared to all other conditions. Furthermore, it revealed a homogeneous distribution pattern of mineralization deposits in contrast to the cell-based scaffolds, where mineralization was only at the surface. Therefore, the secretome of MSCs assembled into 3D-MTs may represent an interesting therapeutic strategy for a next-generation bone healing concept.Lukas OttoPetra WolintAnnina BoppAnna WoloszykAnton S. BeckerAndreas BossRoland BöniMaurizio CalcagniPietro GiovanoliSimon P. HoerstrupMaximilian Y. EmmertJohanna BuschmannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lukas Otto Petra Wolint Annina Bopp Anna Woloszyk Anton S. Becker Andreas Boss Roland Böni Maurizio Calcagni Pietro Giovanoli Simon P. Hoerstrup Maximilian Y. Emmert Johanna Buschmann 3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model
description	Abstract Bone regeneration is a complex process and the clinical translation of tissue engineered constructs (TECs) remains a challenge. The combination of biomaterials and mesenchymal stem cells (MSCs) may enhance the healing process through paracrine effects. Here, we investigated the influence of cell format in combination with a collagen scaffold on key factors in bone healing process, such as mineralization, cell infiltration, vascularization, and ECM production. MSCs as single cells (2D-SCs), assembled into microtissues (3D-MTs) or their corresponding secretomes were combined with a collagen scaffold and incubated on the chicken embryo chorioallantoic membrane (CAM) for 7 days. A comprehensive quantitative analysis was performed on a cellular level by histology and by microcomputed tomography (microCT). In all experimental groups, accumulation of collagen and glycosaminoglycan within the scaffold was observed over time. A pronounced cell infiltration and vascularization from the interface to the surface region of the CAM was detected. The 3D-MT secretome showed a significant mineralization of the biomaterial using microCT compared to all other conditions. Furthermore, it revealed a homogeneous distribution pattern of mineralization deposits in contrast to the cell-based scaffolds, where mineralization was only at the surface. Therefore, the secretome of MSCs assembled into 3D-MTs may represent an interesting therapeutic strategy for a next-generation bone healing concept.
format	article
author	Lukas Otto Petra Wolint Annina Bopp Anna Woloszyk Anton S. Becker Andreas Boss Roland Böni Maurizio Calcagni Pietro Giovanoli Simon P. Hoerstrup Maximilian Y. Emmert Johanna Buschmann
author_facet	Lukas Otto Petra Wolint Annina Bopp Anna Woloszyk Anton S. Becker Andreas Boss Roland Böni Maurizio Calcagni Pietro Giovanoli Simon P. Hoerstrup Maximilian Y. Emmert Johanna Buschmann
author_sort	Lukas Otto
title	3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model
title_short	3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model
title_full	3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model
title_fullStr	3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model
title_full_unstemmed	3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model
title_sort	3d-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/13a7a4e40eaf4c73bc17ec0a47a77cc5
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3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model

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