Transcriptome analysis of Xanthomonas fragariae in strawberry leaves

Abstract Xanthomonas fragariae is a quarantine bacterial pathogen that causes angular leaf spot on strawberry. The aim of our study was to analyse the mechanism of interaction of this bacterium with its host plant at the transcriptome level. For this purpose, mRNAs of X. fragariae growing in Wilbrin...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Joanna Puławska, Monika Kałużna, Wojciech Warabieda, Joël F. Pothier, Michael Gétaz, Jan M. van der Wolf
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/13a9302daa1543b9b575ae8995123b8b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:13a9302daa1543b9b575ae8995123b8b
record_format	dspace
spelling	oai:doaj.org-article:13a9302daa1543b9b575ae8995123b8b2021-12-02T15:09:57ZTranscriptome analysis of Xanthomonas fragariae in strawberry leaves10.1038/s41598-020-77612-y2045-2322https://doaj.org/article/13a9302daa1543b9b575ae8995123b8b2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77612-yhttps://doaj.org/toc/2045-2322Abstract Xanthomonas fragariae is a quarantine bacterial pathogen that causes angular leaf spot on strawberry. The aim of our study was to analyse the mechanism of interaction of this bacterium with its host plant at the transcriptome level. For this purpose, mRNAs of X. fragariae growing in Wilbrink’s medium and from infected strawberry cv. Elsanta plants were isolated and sequenced using the Illumina MiSeq platform. The expression profiles of the bacteria in Wilbrink’s medium and in planta were very diverse. Of the 3939 CDSs recorded, 1995 had significantly different expression in planta (966 and 1029 genes were down- and upregulated, respectively). Among the genes showing increased expression in planta, those with eggNOG/COG (evolutionary genealogy of genes: Non-supervised Orthologous Groups/Cluster of Orthologous Groups) categories associated with bacterial cell motility, signal transduction, transport and metabolism of inorganic ions and carbohydrates and transcription were overrepresented. Among the genes with the most increased expression in planta, genes primarily associated with flagella synthesis and chemotaxis were found. It is also interesting to note that out of the 31 genes localized on a plasmid, 16 were expressed differently in planta, which may indicate their potential role in plant–pathogen interactions. Many genes with differentiated expression that were localized on chromosome and plasmid encode proteins of unknown function.Joanna PuławskaMonika KałużnaWojciech WarabiedaJoël F. PothierMichael GétazJan M. van der WolfNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joanna Puławska Monika Kałużna Wojciech Warabieda Joël F. Pothier Michael Gétaz Jan M. van der Wolf Transcriptome analysis of Xanthomonas fragariae in strawberry leaves
description	Abstract Xanthomonas fragariae is a quarantine bacterial pathogen that causes angular leaf spot on strawberry. The aim of our study was to analyse the mechanism of interaction of this bacterium with its host plant at the transcriptome level. For this purpose, mRNAs of X. fragariae growing in Wilbrink’s medium and from infected strawberry cv. Elsanta plants were isolated and sequenced using the Illumina MiSeq platform. The expression profiles of the bacteria in Wilbrink’s medium and in planta were very diverse. Of the 3939 CDSs recorded, 1995 had significantly different expression in planta (966 and 1029 genes were down- and upregulated, respectively). Among the genes showing increased expression in planta, those with eggNOG/COG (evolutionary genealogy of genes: Non-supervised Orthologous Groups/Cluster of Orthologous Groups) categories associated with bacterial cell motility, signal transduction, transport and metabolism of inorganic ions and carbohydrates and transcription were overrepresented. Among the genes with the most increased expression in planta, genes primarily associated with flagella synthesis and chemotaxis were found. It is also interesting to note that out of the 31 genes localized on a plasmid, 16 were expressed differently in planta, which may indicate their potential role in plant–pathogen interactions. Many genes with differentiated expression that were localized on chromosome and plasmid encode proteins of unknown function.
format	article
author	Joanna Puławska Monika Kałużna Wojciech Warabieda Joël F. Pothier Michael Gétaz Jan M. van der Wolf
author_facet	Joanna Puławska Monika Kałużna Wojciech Warabieda Joël F. Pothier Michael Gétaz Jan M. van der Wolf
author_sort	Joanna Puławska
title	Transcriptome analysis of Xanthomonas fragariae in strawberry leaves
title_short	Transcriptome analysis of Xanthomonas fragariae in strawberry leaves
title_full	Transcriptome analysis of Xanthomonas fragariae in strawberry leaves
title_fullStr	Transcriptome analysis of Xanthomonas fragariae in strawberry leaves
title_full_unstemmed	Transcriptome analysis of Xanthomonas fragariae in strawberry leaves
title_sort	transcriptome analysis of xanthomonas fragariae in strawberry leaves
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/13a9302daa1543b9b575ae8995123b8b
work_keys_str_mv	AT joannapuławska transcriptomeanalysisofxanthomonasfragariaeinstrawberryleaves AT monikakałuzna transcriptomeanalysisofxanthomonasfragariaeinstrawberryleaves AT wojciechwarabieda transcriptomeanalysisofxanthomonasfragariaeinstrawberryleaves AT joelfpothier transcriptomeanalysisofxanthomonasfragariaeinstrawberryleaves AT michaelgetaz transcriptomeanalysisofxanthomonasfragariaeinstrawberryleaves AT janmvanderwolf transcriptomeanalysisofxanthomonasfragariaeinstrawberryleaves
_version_	1718387735548395520

Transcriptome analysis of Xanthomonas fragariae in strawberry leaves

Ejemplares similares