Integrated Lipidomic and Transcriptomic Analysis Reveals Lipid Metabolism in Foxtail Millet (Setaria italica)

Foxtail millet (Setaria italica) as the main traditional crop in China, is rich in many kinds of high quality fatty acids (FAs). In this study, Ultra-high performance liquid chromatography-time-of-flight-tandem mass spectrometer (UHPLC-Q-TOF-MS/MS) was used to determine the lipids of JG35 and JG39....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Haiying Zhang, Junyou Wang, Jing Zhao, Changqing Sun, Jin Wang, Qian Wang, Fei Qu, Xiaodong Yun, Zhiwei Feng
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	foxtail millet UPLC-Q-TOF-MS/MS lipidomics transcriptome association analysis Genetics QH426-470
Acceso en línea:	https://doaj.org/article/13b0f9a70a664a3f87bb8393362b984f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:13b0f9a70a664a3f87bb8393362b984f
record_format	dspace
spelling	oai:doaj.org-article:13b0f9a70a664a3f87bb8393362b984f2021-11-16T06:53:45ZIntegrated Lipidomic and Transcriptomic Analysis Reveals Lipid Metabolism in Foxtail Millet (Setaria italica)1664-802110.3389/fgene.2021.758003https://doaj.org/article/13b0f9a70a664a3f87bb8393362b984f2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.758003/fullhttps://doaj.org/toc/1664-8021Foxtail millet (Setaria italica) as the main traditional crop in China, is rich in many kinds of high quality fatty acids (FAs). In this study, Ultra-high performance liquid chromatography-time-of-flight-tandem mass spectrometer (UHPLC-Q-TOF-MS/MS) was used to determine the lipids of JG35 and JG39. A total of 2,633 lipid molecules and 31 lipid subclasses were identified, mainly including thirteen kinds of glycerophospholipids (GP), eleven kinds of glycerolipids (GL), four kinds of sphingolipids (SP), two kinds of fatty acyls (FA) and one kind of sterol (ST). Among them JG35 had higher contents of diacylglycerols (DG) and ceramides (Cer), while triacylglycerols, phosphatidyl ethanolamine, phosphatidic acid, sterol, fatty acyls and pardiolipin (TG, PE, PA, ST, FA and CL) were higher in JG39. Meantime, the correlation analysis of lipidomics and transcriptomics was used to map the main differential lipid metabolism pathways of foxtail millet. The results shown that a differentially expressed genes (DEGs) of FATA/B for the synthesis of FA was highly expressed in JG35, and the related genes for the synthesis DG (ACCase, KAS, HAD, KCS, LACS and GAPT), TG (DGAT and PDAT) and CL (CLS) were highly expressed in JG39. The results of this study will provide a theoretical basis for the future study of lipidomics, improvement of lipid quality directionally and breeding of idiosyncratic quality varieties in foxtail millet.Haiying ZhangJunyou WangJing ZhaoChangqing SunJin WangQian WangFei QuXiaodong YunZhiwei FengFrontiers Media S.A.articlefoxtail milletUPLC-Q-TOF-MS/MSlipidomicstranscriptomeassociation analysisGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	foxtail millet UPLC-Q-TOF-MS/MS lipidomics transcriptome association analysis Genetics QH426-470
spellingShingle	foxtail millet UPLC-Q-TOF-MS/MS lipidomics transcriptome association analysis Genetics QH426-470 Haiying Zhang Junyou Wang Jing Zhao Changqing Sun Jin Wang Qian Wang Fei Qu Xiaodong Yun Zhiwei Feng Integrated Lipidomic and Transcriptomic Analysis Reveals Lipid Metabolism in Foxtail Millet (Setaria italica)
description	Foxtail millet (Setaria italica) as the main traditional crop in China, is rich in many kinds of high quality fatty acids (FAs). In this study, Ultra-high performance liquid chromatography-time-of-flight-tandem mass spectrometer (UHPLC-Q-TOF-MS/MS) was used to determine the lipids of JG35 and JG39. A total of 2,633 lipid molecules and 31 lipid subclasses were identified, mainly including thirteen kinds of glycerophospholipids (GP), eleven kinds of glycerolipids (GL), four kinds of sphingolipids (SP), two kinds of fatty acyls (FA) and one kind of sterol (ST). Among them JG35 had higher contents of diacylglycerols (DG) and ceramides (Cer), while triacylglycerols, phosphatidyl ethanolamine, phosphatidic acid, sterol, fatty acyls and pardiolipin (TG, PE, PA, ST, FA and CL) were higher in JG39. Meantime, the correlation analysis of lipidomics and transcriptomics was used to map the main differential lipid metabolism pathways of foxtail millet. The results shown that a differentially expressed genes (DEGs) of FATA/B for the synthesis of FA was highly expressed in JG35, and the related genes for the synthesis DG (ACCase, KAS, HAD, KCS, LACS and GAPT), TG (DGAT and PDAT) and CL (CLS) were highly expressed in JG39. The results of this study will provide a theoretical basis for the future study of lipidomics, improvement of lipid quality directionally and breeding of idiosyncratic quality varieties in foxtail millet.
format	article
author	Haiying Zhang Junyou Wang Jing Zhao Changqing Sun Jin Wang Qian Wang Fei Qu Xiaodong Yun Zhiwei Feng
author_facet	Haiying Zhang Junyou Wang Jing Zhao Changqing Sun Jin Wang Qian Wang Fei Qu Xiaodong Yun Zhiwei Feng
author_sort	Haiying Zhang
title	Integrated Lipidomic and Transcriptomic Analysis Reveals Lipid Metabolism in Foxtail Millet (Setaria italica)
title_short	Integrated Lipidomic and Transcriptomic Analysis Reveals Lipid Metabolism in Foxtail Millet (Setaria italica)
title_full	Integrated Lipidomic and Transcriptomic Analysis Reveals Lipid Metabolism in Foxtail Millet (Setaria italica)
title_fullStr	Integrated Lipidomic and Transcriptomic Analysis Reveals Lipid Metabolism in Foxtail Millet (Setaria italica)
title_full_unstemmed	Integrated Lipidomic and Transcriptomic Analysis Reveals Lipid Metabolism in Foxtail Millet (Setaria italica)
title_sort	integrated lipidomic and transcriptomic analysis reveals lipid metabolism in foxtail millet (setaria italica)
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/13b0f9a70a664a3f87bb8393362b984f
work_keys_str_mv	AT haiyingzhang integratedlipidomicandtranscriptomicanalysisrevealslipidmetabolisminfoxtailmilletsetariaitalica AT junyouwang integratedlipidomicandtranscriptomicanalysisrevealslipidmetabolisminfoxtailmilletsetariaitalica AT jingzhao integratedlipidomicandtranscriptomicanalysisrevealslipidmetabolisminfoxtailmilletsetariaitalica AT changqingsun integratedlipidomicandtranscriptomicanalysisrevealslipidmetabolisminfoxtailmilletsetariaitalica AT jinwang integratedlipidomicandtranscriptomicanalysisrevealslipidmetabolisminfoxtailmilletsetariaitalica AT qianwang integratedlipidomicandtranscriptomicanalysisrevealslipidmetabolisminfoxtailmilletsetariaitalica AT feiqu integratedlipidomicandtranscriptomicanalysisrevealslipidmetabolisminfoxtailmilletsetariaitalica AT xiaodongyun integratedlipidomicandtranscriptomicanalysisrevealslipidmetabolisminfoxtailmilletsetariaitalica AT zhiweifeng integratedlipidomicandtranscriptomicanalysisrevealslipidmetabolisminfoxtailmilletsetariaitalica
_version_	1718426649927614464

Integrated Lipidomic and Transcriptomic Analysis Reveals Lipid Metabolism in Foxtail Millet (Setaria italica)

Ejemplares similares