Molecular identification of shark fins in Malaysian Borneo’s local markets

Md-Zain BM, Abid-Kamal SNA, Aifat NR, Abdul-Latiff MAB, Mohd-Hashim A, Ampeng A, Yaakop S, Samat A. 2018. Molecular identification of shark fins in Malaysian Borneo’s local markets. Biodiversitas 19: 1035-1043. A molecular study was carried out to investigate the potential of the cytochrome c oxidas...

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Autores principales:	BADRUL MUNIR MD-ZAIN, SITI NUR AZMINA ABID-KAMAL, NOR RAHMAN AIFAT, MUHAMMAD ABU BAKAR ABDUL-LATIFF, ABU MOHD- HASHIM, AHMAD AMPENG, SALMAH YAAKOP, ABDULLAH SAMAT
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	dna barcode phylogenetic relationships phylogenetic tree shark fin species identification Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/13b4fbeb37dc402988aa8bdc1d4eda20
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spelling	oai:doaj.org-article:13b4fbeb37dc402988aa8bdc1d4eda202021-11-16T14:03:00ZMolecular identification of shark fins in Malaysian Borneo’s local markets1412-033X2085-472210.13057/biodiv/d190336https://doaj.org/article/13b4fbeb37dc402988aa8bdc1d4eda202018-05-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2743https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Md-Zain BM, Abid-Kamal SNA, Aifat NR, Abdul-Latiff MAB, Mohd-Hashim A, Ampeng A, Yaakop S, Samat A. 2018. Molecular identification of shark fins in Malaysian Borneo’s local markets. Biodiversitas 19: 1035-1043. A molecular study was carried out to investigate the potential of the cytochrome c oxidase subunit I (COI) gene as a molecular marker for the genetic identification of shark fin samples that have gone through various preservation processes. A total number of 17 shark fin samples were collected from local markets in Sabah and Sarawak (Malaysian Borneo). The DNA sequences of the 17 samples were amplified by using polymerase chain reaction. The results from the analysis showed that, in the 17 sequences, there were 16 haplotypes present, with 244 sites from 688 bp of the sequences. For phylogeny analysis, tree topologies were reconstructed using the neighbor-joining (NJ) and maximum parsimony (MP) methods. DNA barcoding technique successfully identifies shark fins collected in local markets in Malaysian Borneo at species level employed during this study. Phylogenetic analysis showed that there were four clades that distinguish the four different orders present in the sample species. These clades had bootstrap values higher than 80. In addition, results indicated that 88.2% of the individuals are listed as endangered (Lamiopsis tephrodes, Sphyrna mokarran, and Sphyrna lewini), vulnerable (Alopias pelagicus and Rhynchobatus australiae), and near threatened (Carcharhinus limbatus, Chiloscyllium griseum, Carcharhinus sorrah, and Carcharhinus brevipinna), in the International Union for Conservation of Nature (IUCN) Red Data List.BADRUL MUNIR MD-ZAINSITI NUR AZMINA ABID-KAMALNOR RAHMAN AIFATMUHAMMAD ABU BAKAR ABDUL-LATIFFABU MOHD- HASHIMAHMAD AMPENGSALMAH YAAKOPABDULLAH SAMATMBI & UNS Soloarticledna barcodephylogenetic relationshipsphylogenetic treeshark finspecies identificationBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 3, Pp 1035-1043 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	dna barcode phylogenetic relationships phylogenetic tree shark fin species identification Biology (General) QH301-705.5
spellingShingle	dna barcode phylogenetic relationships phylogenetic tree shark fin species identification Biology (General) QH301-705.5 BADRUL MUNIR MD-ZAIN SITI NUR AZMINA ABID-KAMAL NOR RAHMAN AIFAT MUHAMMAD ABU BAKAR ABDUL-LATIFF ABU MOHD- HASHIM AHMAD AMPENG SALMAH YAAKOP ABDULLAH SAMAT Molecular identification of shark fins in Malaysian Borneo’s local markets
description	Md-Zain BM, Abid-Kamal SNA, Aifat NR, Abdul-Latiff MAB, Mohd-Hashim A, Ampeng A, Yaakop S, Samat A. 2018. Molecular identification of shark fins in Malaysian Borneo’s local markets. Biodiversitas 19: 1035-1043. A molecular study was carried out to investigate the potential of the cytochrome c oxidase subunit I (COI) gene as a molecular marker for the genetic identification of shark fin samples that have gone through various preservation processes. A total number of 17 shark fin samples were collected from local markets in Sabah and Sarawak (Malaysian Borneo). The DNA sequences of the 17 samples were amplified by using polymerase chain reaction. The results from the analysis showed that, in the 17 sequences, there were 16 haplotypes present, with 244 sites from 688 bp of the sequences. For phylogeny analysis, tree topologies were reconstructed using the neighbor-joining (NJ) and maximum parsimony (MP) methods. DNA barcoding technique successfully identifies shark fins collected in local markets in Malaysian Borneo at species level employed during this study. Phylogenetic analysis showed that there were four clades that distinguish the four different orders present in the sample species. These clades had bootstrap values higher than 80. In addition, results indicated that 88.2% of the individuals are listed as endangered (Lamiopsis tephrodes, Sphyrna mokarran, and Sphyrna lewini), vulnerable (Alopias pelagicus and Rhynchobatus australiae), and near threatened (Carcharhinus limbatus, Chiloscyllium griseum, Carcharhinus sorrah, and Carcharhinus brevipinna), in the International Union for Conservation of Nature (IUCN) Red Data List.
format	article
author	BADRUL MUNIR MD-ZAIN SITI NUR AZMINA ABID-KAMAL NOR RAHMAN AIFAT MUHAMMAD ABU BAKAR ABDUL-LATIFF ABU MOHD- HASHIM AHMAD AMPENG SALMAH YAAKOP ABDULLAH SAMAT
author_facet	BADRUL MUNIR MD-ZAIN SITI NUR AZMINA ABID-KAMAL NOR RAHMAN AIFAT MUHAMMAD ABU BAKAR ABDUL-LATIFF ABU MOHD- HASHIM AHMAD AMPENG SALMAH YAAKOP ABDULLAH SAMAT
author_sort	BADRUL MUNIR MD-ZAIN
title	Molecular identification of shark fins in Malaysian Borneo’s local markets
title_short	Molecular identification of shark fins in Malaysian Borneo’s local markets
title_full	Molecular identification of shark fins in Malaysian Borneo’s local markets
title_fullStr	Molecular identification of shark fins in Malaysian Borneo’s local markets
title_full_unstemmed	Molecular identification of shark fins in Malaysian Borneo’s local markets
title_sort	molecular identification of shark fins in malaysian borneo’s local markets
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/13b4fbeb37dc402988aa8bdc1d4eda20
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Molecular identification of shark fins in Malaysian Borneo’s local markets

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