Prenatal polycyclic aromatic hydrocarbon, adiposity, peroxisome proliferator-activated receptor (PPAR) γ methylation in offspring, grand-offspring mice.

<h4>Rationale</h4>Greater levels of prenatal exposure to polycyclic aromatic hydrocarbon (PAH) have been associated with childhood obesity in epidemiological studies. However, the underlying mechanisms are unclear.<h4>Objectives</h4>We hypothesized that prenatal PAH over-expo...

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Autores principales:	Zhonghai Yan, Hanjie Zhang, Christina Maher, Emilio Arteaga-Solis, Frances A Champagne, Licheng Wu, Jacob D McDonald, Beizhan Yan, Gary J Schwartz, Rachel L Miller
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/13b70389bb944b83b6e551fccd338288
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spelling	oai:doaj.org-article:13b70389bb944b83b6e551fccd3382882021-11-25T05:55:15ZPrenatal polycyclic aromatic hydrocarbon, adiposity, peroxisome proliferator-activated receptor (PPAR) γ methylation in offspring, grand-offspring mice.1932-620310.1371/journal.pone.0110706https://doaj.org/article/13b70389bb944b83b6e551fccd3382882014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0110706https://doaj.org/toc/1932-6203<h4>Rationale</h4>Greater levels of prenatal exposure to polycyclic aromatic hydrocarbon (PAH) have been associated with childhood obesity in epidemiological studies. However, the underlying mechanisms are unclear.<h4>Objectives</h4>We hypothesized that prenatal PAH over-exposure during gestation would lead to weight gain and increased fat mass in offspring and grand-offspring mice. Further, we hypothesized that altered adipose gene expression and DNA methylation in genes important to adipocyte differentiation would be affected.<h4>Materials and methods</h4>Pregnant dams were exposed to a nebulized PAH mixture versus negative control aerosol 5 days a week, for 3 weeks. Body weight was recorded from postnatal day (PND) 21 through PND60. Body composition, adipose cell size, gene expression of peroxisome proliferator-activated receptor (PPAR) γ, CCAAT/enhancer-binding proteins (C/EBP) α, cyclooxygenase (Cox)-2, fatty acid synthase (FAS) and adiponectin, and DNA methylation of PPAR γ, were assayed in both the offspring and grand-offspring adipose tissue.<h4>Findings</h4>Offspring of dams exposed to greater PAH during gestation had increased weight, fat mass, as well as higher gene expression of PPAR γ, C/EBP α, Cox2, FAS and adiponectin and lower DNA methylation of PPAR γ. Similar differences in phenotype and DNA methylation extended through the grand-offspring mice.<h4>Conclusions</h4>Greater prenatal PAH exposure was associated with increased weight, fat mass, adipose gene expression and epigenetic changes in progeny.Zhonghai YanHanjie ZhangChristina MaherEmilio Arteaga-SolisFrances A ChampagneLicheng WuJacob D McDonaldBeizhan YanGary J SchwartzRachel L MillerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e110706 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhonghai Yan Hanjie Zhang Christina Maher Emilio Arteaga-Solis Frances A Champagne Licheng Wu Jacob D McDonald Beizhan Yan Gary J Schwartz Rachel L Miller Prenatal polycyclic aromatic hydrocarbon, adiposity, peroxisome proliferator-activated receptor (PPAR) γ methylation in offspring, grand-offspring mice.
description	<h4>Rationale</h4>Greater levels of prenatal exposure to polycyclic aromatic hydrocarbon (PAH) have been associated with childhood obesity in epidemiological studies. However, the underlying mechanisms are unclear.<h4>Objectives</h4>We hypothesized that prenatal PAH over-exposure during gestation would lead to weight gain and increased fat mass in offspring and grand-offspring mice. Further, we hypothesized that altered adipose gene expression and DNA methylation in genes important to adipocyte differentiation would be affected.<h4>Materials and methods</h4>Pregnant dams were exposed to a nebulized PAH mixture versus negative control aerosol 5 days a week, for 3 weeks. Body weight was recorded from postnatal day (PND) 21 through PND60. Body composition, adipose cell size, gene expression of peroxisome proliferator-activated receptor (PPAR) γ, CCAAT/enhancer-binding proteins (C/EBP) α, cyclooxygenase (Cox)-2, fatty acid synthase (FAS) and adiponectin, and DNA methylation of PPAR γ, were assayed in both the offspring and grand-offspring adipose tissue.<h4>Findings</h4>Offspring of dams exposed to greater PAH during gestation had increased weight, fat mass, as well as higher gene expression of PPAR γ, C/EBP α, Cox2, FAS and adiponectin and lower DNA methylation of PPAR γ. Similar differences in phenotype and DNA methylation extended through the grand-offspring mice.<h4>Conclusions</h4>Greater prenatal PAH exposure was associated with increased weight, fat mass, adipose gene expression and epigenetic changes in progeny.
format	article
author	Zhonghai Yan Hanjie Zhang Christina Maher Emilio Arteaga-Solis Frances A Champagne Licheng Wu Jacob D McDonald Beizhan Yan Gary J Schwartz Rachel L Miller
author_facet	Zhonghai Yan Hanjie Zhang Christina Maher Emilio Arteaga-Solis Frances A Champagne Licheng Wu Jacob D McDonald Beizhan Yan Gary J Schwartz Rachel L Miller
author_sort	Zhonghai Yan
title	Prenatal polycyclic aromatic hydrocarbon, adiposity, peroxisome proliferator-activated receptor (PPAR) γ methylation in offspring, grand-offspring mice.
title_short	Prenatal polycyclic aromatic hydrocarbon, adiposity, peroxisome proliferator-activated receptor (PPAR) γ methylation in offspring, grand-offspring mice.
title_full	Prenatal polycyclic aromatic hydrocarbon, adiposity, peroxisome proliferator-activated receptor (PPAR) γ methylation in offspring, grand-offspring mice.
title_fullStr	Prenatal polycyclic aromatic hydrocarbon, adiposity, peroxisome proliferator-activated receptor (PPAR) γ methylation in offspring, grand-offspring mice.
title_full_unstemmed	Prenatal polycyclic aromatic hydrocarbon, adiposity, peroxisome proliferator-activated receptor (PPAR) γ methylation in offspring, grand-offspring mice.
title_sort	prenatal polycyclic aromatic hydrocarbon, adiposity, peroxisome proliferator-activated receptor (ppar) γ methylation in offspring, grand-offspring mice.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/13b70389bb944b83b6e551fccd338288
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Prenatal polycyclic aromatic hydrocarbon, adiposity, peroxisome proliferator-activated receptor (PPAR) γ methylation in offspring, grand-offspring mice.

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