<italic toggle="yes">MoGT2</italic> Is Essential for Morphogenesis and Pathogenicity of <named-content content-type="genus-species">Magnaporthe oryzae</named-content>

ABSTRACT Magnaporthe oryzae causes the rice blast disease, which is one of the most serious diseases of cultivated rice worldwide. Glycosylation is an important posttranslational modification of secretory and membrane proteins in all eukaryotes, catalyzed by glycosyltransferases (GTs). In this study...

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Autores principales:	Shuzhen Deng, Wenda Sun, Lihong Dong, Guobing Cui, Yi Zhen Deng
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	Magnaporthe oryzae MoGt2 pathogenesis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/13bb8c98507245f0a7f3751de7a60941
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spelling	oai:doaj.org-article:13bb8c98507245f0a7f3751de7a609412021-11-15T15:27:33Z<italic toggle="yes">MoGT2</italic> Is Essential for Morphogenesis and Pathogenicity of <named-content content-type="genus-species">Magnaporthe oryzae</named-content>10.1128/mSphere.00309-192379-5042https://doaj.org/article/13bb8c98507245f0a7f3751de7a609412019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00309-19https://doaj.org/toc/2379-5042ABSTRACT Magnaporthe oryzae causes the rice blast disease, which is one of the most serious diseases of cultivated rice worldwide. Glycosylation is an important posttranslational modification of secretory and membrane proteins in all eukaryotes, catalyzed by glycosyltransferases (GTs). In this study, we identified and characterized a type 2 glycosyltransferase, MoGt2, in M. oryzae. Targeted gene deletion mutants of MoGT2 (mogt2Δ strains) were nonpathogenic and were impaired in vegetative growth, conidiation, and appressorium formation at hyphal tips. Moreover, MoGT2 plays an important role in stress tolerance and hydrophobin function of M. oryzae. Site-directed mutagenesis analysis showed that conserved glycosyltransferase domains (DxD and QxxRW) are critical for biological functions of MoGt2. MoGT2 deletion led to altered glycoproteins during M. oryzae conidiation. By liquid chromatography-tandem mass spectrometry (LC-MS/MS), we identified several candidate proteins as potential substrates of MoGt2, including several heat shock proteins, two coiled-coil domain-containing proteins, aminopeptidase 2, and nuclease domain-containing protein 1. On the other hand, we found that a conidiation-related gene, genes involved in various metabolism pathways, and genes involved in cell wall integrity and/or osmotic response were differentially regulated in the mogt2Δ mutant, which may potentially contribute to its condiation defects. Taken together, our results show that MoGt2 is important for infection-related morphogenesis and pathogenesis in M. oryzae. IMPORTANCE The ascomycete fungus Magnapothe oryzae is the causal agent of rice blast disease, leading to severe loss in cultivated rice production worldwide. In this study, we identified a conserved type 2 glycosyltransferase named MoGt2 in M. oryzae. The mogt2Δ targeted gene deletion mutants exhibited pleiotropic defects in vegetative growth, conidiation, stress response, hyphal appressorium-mediated penetration, and pathogenicity. Furthermore, conserved glycosyltransferase domains are critical for MoGt2 function. The comparative transcriptome analysis revealed potential target genes under MoGt2 regulation in M. oryzae conidiation. Identification of potential glycoproteins modified by MoGt2 provided information on its regulatory mechanism of gene expression and biological functions. Overall, our study represents the first report of type 2 glycosyltransferase function in M. oryzae infection-related morphogenesis and pathogenesis.Shuzhen DengWenda SunLihong DongGuobing CuiYi Zhen DengAmerican Society for MicrobiologyarticleMagnaporthe oryzaeMoGt2pathogenesisMicrobiologyQR1-502ENmSphere, Vol 4, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Magnaporthe oryzae MoGt2 pathogenesis Microbiology QR1-502
spellingShingle	Magnaporthe oryzae MoGt2 pathogenesis Microbiology QR1-502 Shuzhen Deng Wenda Sun Lihong Dong Guobing Cui Yi Zhen Deng <italic toggle="yes">MoGT2</italic> Is Essential for Morphogenesis and Pathogenicity of <named-content content-type="genus-species">Magnaporthe oryzae</named-content>
description	ABSTRACT Magnaporthe oryzae causes the rice blast disease, which is one of the most serious diseases of cultivated rice worldwide. Glycosylation is an important posttranslational modification of secretory and membrane proteins in all eukaryotes, catalyzed by glycosyltransferases (GTs). In this study, we identified and characterized a type 2 glycosyltransferase, MoGt2, in M. oryzae. Targeted gene deletion mutants of MoGT2 (mogt2Δ strains) were nonpathogenic and were impaired in vegetative growth, conidiation, and appressorium formation at hyphal tips. Moreover, MoGT2 plays an important role in stress tolerance and hydrophobin function of M. oryzae. Site-directed mutagenesis analysis showed that conserved glycosyltransferase domains (DxD and QxxRW) are critical for biological functions of MoGt2. MoGT2 deletion led to altered glycoproteins during M. oryzae conidiation. By liquid chromatography-tandem mass spectrometry (LC-MS/MS), we identified several candidate proteins as potential substrates of MoGt2, including several heat shock proteins, two coiled-coil domain-containing proteins, aminopeptidase 2, and nuclease domain-containing protein 1. On the other hand, we found that a conidiation-related gene, genes involved in various metabolism pathways, and genes involved in cell wall integrity and/or osmotic response were differentially regulated in the mogt2Δ mutant, which may potentially contribute to its condiation defects. Taken together, our results show that MoGt2 is important for infection-related morphogenesis and pathogenesis in M. oryzae. IMPORTANCE The ascomycete fungus Magnapothe oryzae is the causal agent of rice blast disease, leading to severe loss in cultivated rice production worldwide. In this study, we identified a conserved type 2 glycosyltransferase named MoGt2 in M. oryzae. The mogt2Δ targeted gene deletion mutants exhibited pleiotropic defects in vegetative growth, conidiation, stress response, hyphal appressorium-mediated penetration, and pathogenicity. Furthermore, conserved glycosyltransferase domains are critical for MoGt2 function. The comparative transcriptome analysis revealed potential target genes under MoGt2 regulation in M. oryzae conidiation. Identification of potential glycoproteins modified by MoGt2 provided information on its regulatory mechanism of gene expression and biological functions. Overall, our study represents the first report of type 2 glycosyltransferase function in M. oryzae infection-related morphogenesis and pathogenesis.
format	article
author	Shuzhen Deng Wenda Sun Lihong Dong Guobing Cui Yi Zhen Deng
author_facet	Shuzhen Deng Wenda Sun Lihong Dong Guobing Cui Yi Zhen Deng
author_sort	Shuzhen Deng
title	<italic toggle="yes">MoGT2</italic> Is Essential for Morphogenesis and Pathogenicity of <named-content content-type="genus-species">Magnaporthe oryzae</named-content>
title_short	<italic toggle="yes">MoGT2</italic> Is Essential for Morphogenesis and Pathogenicity of <named-content content-type="genus-species">Magnaporthe oryzae</named-content>
title_full	<italic toggle="yes">MoGT2</italic> Is Essential for Morphogenesis and Pathogenicity of <named-content content-type="genus-species">Magnaporthe oryzae</named-content>
title_fullStr	<italic toggle="yes">MoGT2</italic> Is Essential for Morphogenesis and Pathogenicity of <named-content content-type="genus-species">Magnaporthe oryzae</named-content>
title_full_unstemmed	<italic toggle="yes">MoGT2</italic> Is Essential for Morphogenesis and Pathogenicity of <named-content content-type="genus-species">Magnaporthe oryzae</named-content>
title_sort	<italic toggle="yes">mogt2</italic> is essential for morphogenesis and pathogenicity of <named-content content-type="genus-species">magnaporthe oryzae</named-content>
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/13bb8c98507245f0a7f3751de7a60941
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<italic toggle="yes">MoGT2</italic> Is Essential for Morphogenesis and Pathogenicity of <named-content content-type="genus-species">Magnaporthe oryzae</named-content>

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