Common Features and Intra-Species Variation of <i>Cutibacterium modestum</i> Strains, and Emended Description of the Species
<i>Cutibacterium modestum</i> is a new species coined in 2020 as the fifth species of genus <i>Cutibacterium</i>, which includes <i>Cutibacterium acnes</i>. The species is predicted as a minor but common member of skin microbiome and includes a group tentatively n...
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| Autores principales: | , , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/13bce138b67b4924a3968582572d42bc |
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| Sumario: | <i>Cutibacterium modestum</i> is a new species coined in 2020 as the fifth species of genus <i>Cutibacterium</i>, which includes <i>Cutibacterium acnes</i>. The species is predicted as a minor but common member of skin microbiome and includes a group tentatively named as “<i>Propionibacterium humerusii</i>”. The description of the species has been provided only with a single strain. To establish the characteristics of <i>C. modestum</i> and search for possible disease-related subtypes, we investigated the biochemical characteristics of eight live strains and performed in silico comparison of nine genomes. The common features, which included the morphology of Gram-stain positive short rods, the negativity of phenylalanine arylamidase, and several unique MALDI-TOF MS spectral peaks, were considered useful in laboratory identification. Pairwise comparisons of the genomes by in silico DNA–DNA hybridization showed similarity values of 98.1% or larger, which were far higher than the subspecies cutoff of 79–80%. The <i>16S rRNA</i> gene sequences of thirteen isolates and genomes were identical. Their <i>recA</i> gene sequences were identical except for two strains, HM-510 (HL037PA2) and Marseille-P5998, which showed unique one-nucleotide polymorphisms. The biochemical features using API kits were slightly different among the isolates but far closer than those of the nearest other species, <i>C. acnes</i> and <i>Cutibacterium namnetense</i>. Spectra of MALDI-TOF mass spectrometry showed slight differences in the presence of <i>m</i>/<i>z</i> 10,512 (10 kD chaperonin GroS) and three other peaks, further clustering the eight isolates into three subtypes. These results indicated that these isolates did not separate to form subspecies-level clusters, but subtyping is possible by using <i>recA</i> gene sequences or MALDI-TOF mass spectrometry spectra. Moreover, this work has confirmed that a group “<i>P. humerusii</i>” is included in <i>C. modestum</i>. |
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