Virus-induced gene silencing (VIGS) in cape gooseberry (Physalis peruviana L., Solanaceae)
Cape gooseberry (Physalis peruviana, L.) is a herbaceous plant belonging to the Solanaceae family that produces an edible berry appreciated for its nutraceutical and pharmaceutical properties. Its production is often limited by diseases and reproducible fruit quality. Recent studies have reported...
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| Formato: | article |
| Lenguaje: | EN ES |
| Publicado: |
Pontificia Universidad Javeriana
2019
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/13be2074ce004c10b232e14cbcb78c5c |
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| Sumario: | Cape gooseberry (Physalis peruviana, L.) is a herbaceous plant belonging
to the Solanaceae family that produces an edible berry appreciated for its
nutraceutical and pharmaceutical properties. Its production is often limited
by diseases and reproducible fruit quality. Recent studies have reported genes
associated with fruit quality and resistance response to the root-infecting
fungus Fusarium oxysporum f. sp. physali (Foph,) which causes vascular wilt.
In order to standardize a method to validate the biological function of
candidate genes in the non-model species P. peruviana, we tested the robust
approach in reverse genetics, virus-induced gene silencing (VIGS). In this
study, we validated and optimized VIGS using an insert of the phytoene
desaturase (PDS) gene in a silencing viral vector generated from tobacco
rattle virus (TRV). Leaves infiltrated with Agrobacterium (GV3101 strain)
showed photo-bleached segments, which were distinctive for PDS suppression
at 7 days post-infection (dpi). More than half of the treated plants showed
photo-bleaching, indicating an efficiency rate of 50 % of the VIGS protocol.
The results of this study showed that VIGS can be used for future functional
gene characterization implicated in the immune response, disease resistance
and fruit quality in capegooseberry.
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