Virus-induced gene silencing (VIGS) in cape gooseberry (Physalis peruviana L., Solanaceae)
Cape gooseberry (Physalis peruviana, L.) is a herbaceous plant belonging to the Solanaceae family that produces an edible berry appreciated for its nutraceutical and pharmaceutical properties. Its production is often limited by diseases and reproducible fruit quality. Recent studies have reported...
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Pontificia Universidad Javeriana
2019
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oai:doaj.org-article:13be2074ce004c10b232e14cbcb78c5c2021-11-16T14:55:13ZVirus-induced gene silencing (VIGS) in cape gooseberry (Physalis peruviana L., Solanaceae)10.11144/Javeriana.SC24-1.vigs0122-74832027-1352https://doaj.org/article/13be2074ce004c10b232e14cbcb78c5c2019-04-01T00:00:00Zhttps://revistas.javeriana.edu.co/index.php/scientarium/article/view/21347https://doaj.org/toc/0122-7483https://doaj.org/toc/2027-1352Cape gooseberry (Physalis peruviana, L.) is a herbaceous plant belonging to the Solanaceae family that produces an edible berry appreciated for its nutraceutical and pharmaceutical properties. Its production is often limited by diseases and reproducible fruit quality. Recent studies have reported genes associated with fruit quality and resistance response to the root-infecting fungus Fusarium oxysporum f. sp. physali (Foph,) which causes vascular wilt. In order to standardize a method to validate the biological function of candidate genes in the non-model species P. peruviana, we tested the robust approach in reverse genetics, virus-induced gene silencing (VIGS). In this study, we validated and optimized VIGS using an insert of the phytoene desaturase (PDS) gene in a silencing viral vector generated from tobacco rattle virus (TRV). Leaves infiltrated with Agrobacterium (GV3101 strain) showed photo-bleached segments, which were distinctive for PDS suppression at 7 days post-infection (dpi). More than half of the treated plants showed photo-bleaching, indicating an efficiency rate of 50 % of the VIGS protocol. The results of this study showed that VIGS can be used for future functional gene characterization implicated in the immune response, disease resistance and fruit quality in capegooseberry. Jaime A. Osorio-Guarín, Francy L. García-Arias, Roxana YocktengPontificia Universidad Javerianaarticle: physalis peruviana; phytoene desaturase; post-transcriptional gene silencing; tobacco rattle virus; virus induced gene silencing.Science (General)Q1-390ENESUniversitas Scientiarum, Vol 24, Iss 1, Pp 111-133 (2019) |
| institution |
DOAJ |
| collection |
DOAJ |
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EN ES |
| topic |
: physalis peruviana; phytoene desaturase; post-transcriptional gene silencing; tobacco rattle virus; virus induced gene silencing. Science (General) Q1-390 |
| spellingShingle |
: physalis peruviana; phytoene desaturase; post-transcriptional gene silencing; tobacco rattle virus; virus induced gene silencing. Science (General) Q1-390 Jaime A. Osorio-Guarín, Francy L. García-Arias, Roxana Yockteng Virus-induced gene silencing (VIGS) in cape gooseberry (Physalis peruviana L., Solanaceae) |
| description |
Cape gooseberry (Physalis peruviana, L.) is a herbaceous plant belonging
to the Solanaceae family that produces an edible berry appreciated for its
nutraceutical and pharmaceutical properties. Its production is often limited
by diseases and reproducible fruit quality. Recent studies have reported genes
associated with fruit quality and resistance response to the root-infecting
fungus Fusarium oxysporum f. sp. physali (Foph,) which causes vascular wilt.
In order to standardize a method to validate the biological function of
candidate genes in the non-model species P. peruviana, we tested the robust
approach in reverse genetics, virus-induced gene silencing (VIGS). In this
study, we validated and optimized VIGS using an insert of the phytoene
desaturase (PDS) gene in a silencing viral vector generated from tobacco
rattle virus (TRV). Leaves infiltrated with Agrobacterium (GV3101 strain)
showed photo-bleached segments, which were distinctive for PDS suppression
at 7 days post-infection (dpi). More than half of the treated plants showed
photo-bleaching, indicating an efficiency rate of 50 % of the VIGS protocol.
The results of this study showed that VIGS can be used for future functional
gene characterization implicated in the immune response, disease resistance
and fruit quality in capegooseberry.
|
| format |
article |
| author |
Jaime A. Osorio-Guarín, Francy L. García-Arias, Roxana Yockteng |
| author_facet |
Jaime A. Osorio-Guarín, Francy L. García-Arias, Roxana Yockteng |
| author_sort |
Jaime A. Osorio-Guarín, Francy L. García-Arias, Roxana Yockteng |
| title |
Virus-induced gene silencing (VIGS) in cape gooseberry (Physalis peruviana L., Solanaceae) |
| title_short |
Virus-induced gene silencing (VIGS) in cape gooseberry (Physalis peruviana L., Solanaceae) |
| title_full |
Virus-induced gene silencing (VIGS) in cape gooseberry (Physalis peruviana L., Solanaceae) |
| title_fullStr |
Virus-induced gene silencing (VIGS) in cape gooseberry (Physalis peruviana L., Solanaceae) |
| title_full_unstemmed |
Virus-induced gene silencing (VIGS) in cape gooseberry (Physalis peruviana L., Solanaceae) |
| title_sort |
virus-induced gene silencing (vigs) in cape gooseberry (physalis peruviana l., solanaceae) |
| publisher |
Pontificia Universidad Javeriana |
| publishDate |
2019 |
| url |
https://doaj.org/article/13be2074ce004c10b232e14cbcb78c5c |
| work_keys_str_mv |
AT jaimeaosorioguarinfrancylgarciaariasroxanayockteng virusinducedgenesilencingvigsincapegooseberryphysalisperuvianalsolanaceae |
| _version_ |
1718426343772782592 |