Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2

Abstract Background Current assays for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rely on time consuming, costly and laboratory based methods for virus isolation, purification and removing inhibitors. To address this limitation, we propose a simple method for testing R...

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Autores principales:	Meng Yee Lai, Fatma Diyana Mohd Bukhari, Nur Zulaikha Zulkefli, Ilyiana Ismail, Nur Izati Mustapa, Tuan Suhaila Tuan Soh, Afifah Haji Hassan, Kalaiarasu M. Peariasamy, Yee Leng Lee, Jeyanthi Suppiah, Ravindran Thayan, Yee Ling Lau
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	SARS-CoV-2 Isothermal detection Coronaviruses Rapid diagnosis RT-LAMP Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/13bfea2ade1a4c28b667c75d495423da
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spelling	oai:doaj.org-article:13bfea2ade1a4c28b667c75d495423da2021-11-21T12:42:41ZTwo extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-210.1186/s12879-021-06876-01471-2334https://doaj.org/article/13bfea2ade1a4c28b667c75d495423da2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12879-021-06876-0https://doaj.org/toc/1471-2334Abstract Background Current assays for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rely on time consuming, costly and laboratory based methods for virus isolation, purification and removing inhibitors. To address this limitation, we propose a simple method for testing RNA from nasopharyngeal swab samples that bypasses the RNA purification step. Methods In the current project, we have described two extraction-free reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for the detection of SARS-CoV-2 by using E gene and RdRp gene as the targets. Results Here, results showed that reverse transcription loop-mediated isothermal amplification assays with 88.4% sensitive (95% CI: 74.9–96.1%) and 67.4% sensitive (95% CI: 51.5–80.9%) for E gene and RdRp gene, respectively. Conclusion Without the need of RNA purification, our developed RT-LAMP assays for direct detection of SARS-CoV-2 from nasopharyngeal swab samples could be turned into alternatives to qRT-PCR for rapid screening.Meng Yee LaiFatma Diyana Mohd BukhariNur Zulaikha ZulkefliIlyiana IsmailNur Izati MustapaTuan Suhaila Tuan SohAfifah Haji HassanKalaiarasu M. PeariasamyYee Leng LeeJeyanthi SuppiahRavindran ThayanYee Ling LauBMCarticleSARS-CoV-2Isothermal detectionCoronavirusesRapid diagnosisRT-LAMPInfectious and parasitic diseasesRC109-216ENBMC Infectious Diseases, Vol 21, Iss 1, Pp 1-5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 Isothermal detection Coronaviruses Rapid diagnosis RT-LAMP Infectious and parasitic diseases RC109-216
spellingShingle	SARS-CoV-2 Isothermal detection Coronaviruses Rapid diagnosis RT-LAMP Infectious and parasitic diseases RC109-216 Meng Yee Lai Fatma Diyana Mohd Bukhari Nur Zulaikha Zulkefli Ilyiana Ismail Nur Izati Mustapa Tuan Suhaila Tuan Soh Afifah Haji Hassan Kalaiarasu M. Peariasamy Yee Leng Lee Jeyanthi Suppiah Ravindran Thayan Yee Ling Lau Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2
description	Abstract Background Current assays for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rely on time consuming, costly and laboratory based methods for virus isolation, purification and removing inhibitors. To address this limitation, we propose a simple method for testing RNA from nasopharyngeal swab samples that bypasses the RNA purification step. Methods In the current project, we have described two extraction-free reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for the detection of SARS-CoV-2 by using E gene and RdRp gene as the targets. Results Here, results showed that reverse transcription loop-mediated isothermal amplification assays with 88.4% sensitive (95% CI: 74.9–96.1%) and 67.4% sensitive (95% CI: 51.5–80.9%) for E gene and RdRp gene, respectively. Conclusion Without the need of RNA purification, our developed RT-LAMP assays for direct detection of SARS-CoV-2 from nasopharyngeal swab samples could be turned into alternatives to qRT-PCR for rapid screening.
format	article
author	Meng Yee Lai Fatma Diyana Mohd Bukhari Nur Zulaikha Zulkefli Ilyiana Ismail Nur Izati Mustapa Tuan Suhaila Tuan Soh Afifah Haji Hassan Kalaiarasu M. Peariasamy Yee Leng Lee Jeyanthi Suppiah Ravindran Thayan Yee Ling Lau
author_facet	Meng Yee Lai Fatma Diyana Mohd Bukhari Nur Zulaikha Zulkefli Ilyiana Ismail Nur Izati Mustapa Tuan Suhaila Tuan Soh Afifah Haji Hassan Kalaiarasu M. Peariasamy Yee Leng Lee Jeyanthi Suppiah Ravindran Thayan Yee Ling Lau
author_sort	Meng Yee Lai
title	Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2
title_short	Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2
title_full	Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2
title_fullStr	Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2
title_full_unstemmed	Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2
title_sort	two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of sars-cov-2
publisher	BMC
publishDate	2021
url	https://doaj.org/article/13bfea2ade1a4c28b667c75d495423da
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Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2

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