Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2

Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2

Abstract Background Current assays for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rely on time consuming, costly and laboratory based methods for virus isolation, purification and removing inhibitors. To address this limitation, we propose a simple method for testing R...

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Main Authors: Meng Yee Lai, Fatma Diyana Mohd Bukhari, Nur Zulaikha Zulkefli, Ilyiana Ismail, Nur Izati Mustapa, Tuan Suhaila Tuan Soh, Afifah Haji Hassan, Kalaiarasu M. Peariasamy, Yee Leng Lee, Jeyanthi Suppiah, Ravindran Thayan, Yee Ling Lau
Format: article
Language:EN
Published: BMC 2021
Subjects:
SARS-CoV-2
Isothermal detection
Coronaviruses
Rapid diagnosis
RT-LAMP
Infectious and parasitic diseases
RC109-216
Online Access:https://doaj.org/article/13bfea2ade1a4c28b667c75d495423da
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https://doaj.org/article/13bfea2ade1a4c28b667c75d495423da

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